Production of basement membrane components by a reconstructed epidermis cultured in the absence of serum and dermal factors

Rosdy, Martin; Pisani, A.; Ortonne, Jean‐Paul

doi:10.1111/j.1365-2133.1993.tb11839.x

Cited by 62 publications

(42 citation statements)

References 37 publications

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“…5A). As previously shown in similar experiments performed with normal human keratinocytes (27), laminin-5 accumulated on the inert surface and eventually diffused inside the filter.…”

Section: Polarized Secretion Of Recombinant Laminin-5 By Lsv5-rsupporting

confidence: 55%

“…Filters were kept immersed 7 days in KSM medium to allow formation of a confluent monolayer. Cultures were then raised at the liquid-air interface for 7 days to induce the formation of a differentiated epidermis (27).…”

Section: Methodsmentioning

confidence: 99%

“…Restored LSV5-R keratinocytes were isolated that re-expressed and deposited laminin-5 on the tissue culture substrate and the mesenchyme of nude mice grafted with the transduced cells as observed with human keratinocytes producing functional molecules of this adhesion ligand (16,27). LSV5-R keratinocytes also assumed an epithelial-like shape correlating with changes in the distribution of focal adhesions and a tighter adhesion of the basal plasma membrane to substrate.…”

Section: Synthesis Of Endogenous Laminin-5 Reduces Cell Motilitykeratmentioning

confidence: 99%

“…Cultures of LSV5-R cells were grown on cellulose acetate filters and exposed to air to obtain stratification into a multilayered epithelium (27). Immunofluorescence analysis of these artificial epithelia using mAb GB3 demonstrated that LSV5-R cells synthesize immunoreactive laminin-5 that is actively secreted in a polarized way and deposited on the culture substrate (Fig.…”

Section: Polarized Secretion Of Recombinant Laminin-5 By Lsv5-rmentioning

confidence: 99%

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Functional Re-expression of Laminin-5 in Laminin-γ2-deficient Human Keratinocytes Modifies Cell Morphology, Motility, and Adhesion

Gagnoux‐Palacios

Vailly

Durand-Clément

et al. 1996

Journal of Biological Chemistry

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“…5A). As previously shown in similar experiments performed with normal human keratinocytes (27), laminin-5 accumulated on the inert surface and eventually diffused inside the filter.…”

Section: Polarized Secretion Of Recombinant Laminin-5 By Lsv5-rsupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Section: Synthesis Of Endogenous Laminin-5 Reduces Cell Motilitykeratmentioning

confidence: 99%

Section: Polarized Secretion Of Recombinant Laminin-5 By Lsv5-rmentioning

confidence: 99%

See 2 more Smart Citations

Functional Re-expression of Laminin-5 in Laminin-γ2-deficient Human Keratinocytes Modifies Cell Morphology, Motility, and Adhesion

Gagnoux‐Palacios

Vailly

Durand-Clément

et al. 1996

Journal of Biological Chemistry

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“…Epithelium of skin equivalent forms a multilayered structure which is similar to that of normal skin [11,12,15,34]. In addition, skin equivalent also forms basement membrane including the expression of α 6 β 4 integrin, laminin, and type IV and VII collagens [2,4,12,20,29].…”

mentioning

confidence: 99%

Three-Dimensional Culture of Keratinocytes and the Formation of Basement Membrane for Canine Footpad Substitute

Yamazoe

Miyamoto

Hikosaka

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. A pad equivalent for a dog was prepared as a substitute for the loss of footpad. In addition to the time course of formation on epidermal morphogenesis, we investigated expressions of α 6 integrin subunit as adhesive molecule, and laminin and type IV and VII collagens as extracellular matrices of basement membrane components. Epithelium of the pad equivalent was thick enough to be easily confirmed at 5 days at the air-liquid interface, but many creases appeared on it at 7 days, and it shrank at 10 and 14 days. Keratinocytes were increased in 4 to 5 cell layers at 1 day at the air-liquid interface, differentiating into basal cell layer. Granular and corneal cell layers were confirmed until 5 days, and maintained their shape at least until 14 days. Alpha 6 integrin was expressed at almost the same fluorescent intensity as native pad tissue at 1 day at the dermal-epidermal junction. Laminin and type IV collagen were intermittently expressed at 5 and 10 days, respectively, at the dermal-epidermal junction, and at 14 days the fluorescence showed almost the same intensity as native pad tissue. The expression of type VII collagen was discontinuous at 2 days at the dermal-epidermal junction, but remained as it was at 14 days. The present findings suggested that although the formation of anchoring fibrils in basement membrane was incomplete, the pad equivalent in the dog was reconstructed similar to a native pad by epidermal morphogenesis. KEY WORDS: air-liquid interface, canine, epidermis, footpad, keratinocyte.

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Engineered Skin Tissue Equivalents for Product Evaluation and Therapeutic Applications

Suhail

Sardashti

Jaiswal

et al. 2019

Biotechnology Journal

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The current status of skin tissue equivalents that have emerged as relevant tools in commercial and therapeutic product development applications is reviewed. Due to the rise of animal welfare concerns, numerous companies have designed skin model alternatives to assess the efficacy of pharmaceutical, skincare, and cosmetic products in an in vitro setting, decreasing the dependency on such methods. Skin models have also made an impact in determining the root causes of skin diseases. When designing a skin model, there are various chemical and physical considerations that need to be considered to produce a biomimetic design. This includes designing a structure that mimics the structural characteristics and mechanical strength needed for tribological property measurement and toxicological testing. Recently, various commercial products have made significant progress towards achieving a native skin alternative. Further research involve the development of a functional bilayered model that mimics the constituent properties of the native epidermis and dermis. In this article, the skin models are divided into three categories: in vitro epidermal skin equivalents, in vitro full‐thickness skin equivalents, and clinical skin equivalents. A description of skin model characteristics, testing methods, applications, and potential improvements is presented.

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Production of basement membrane components by a reconstructed epidermis cultured in the absence of serum and dermal factors

Cited by 62 publications

References 37 publications

Functional Re-expression of Laminin-5 in Laminin-γ2-deficient Human Keratinocytes Modifies Cell Morphology, Motility, and Adhesion

Functional Re-expression of Laminin-5 in Laminin-γ2-deficient Human Keratinocytes Modifies Cell Morphology, Motility, and Adhesion

Three-Dimensional Culture of Keratinocytes and the Formation of Basement Membrane for Canine Footpad Substitute

Engineered Skin Tissue Equivalents for Product Evaluation and Therapeutic Applications

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