Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut

Ahmad, Waqar; Nasir, Anam; Sattar, Fazal; Ashfaq, Iram; Chen, Ming Hsu; Hayat, Azam; Rehman, Mujaddad Ur; Zhao, Sainan; Khaliq, Shazia; Ghauri, Muhammad Afzal; Anwar, Munir A.

doi:10.1007/s12223-021-00913-w

Cited by 15 publications

(12 citation statements)

References 56 publications

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“…For obtaining maximum yield of the EPS, different environmental parameters including aeration conditions, pH, temperature and substrate concentration were studied by the method previously used [ 76 ].…”

Section: Methodsmentioning

confidence: 99%

High-Molecular-Weight Dextran-Type Exopolysaccharide Produced by the Novel Apilactobacillus waqarii Improves Metabolic Syndrome: In Vitro and In Vivo Analyses

Ahmad

Boyajian

Abosalha

et al. 2022

IJMS

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Metabolic syndrome is a leading medical concern that affects one billion people worldwide. Metabolic syndrome is defined by a clustering of risk factors that predispose an individual to cardiovascular disease, diabetes and stroke. In recent years, the apparent role of the gut microbiota in metabolic syndrome has drawn attention to microbiome-engineered therapeutics. Specifically, lactic acid bacteria (LAB) harbors beneficial metabolic characteristics, including the production of exopolysaccharides and other microbial byproducts. We recently isolated a novel fructophilic lactic acid bacterium (FLAB), Apilactobacillus waqarii strain HBW1, from honeybee gut and found it produces a dextran-type exopolysaccharide (EPS). The objective of this study was to explore the therapeutic potential of the new dextran in relation to metabolic syndrome. Findings revealed the dextran’s ability to improve the viability of damaged HT-29 intestinal epithelial cells and exhibit antioxidant properties. In vivo analyses demonstrated reductions in body weight gain and serum cholesterol levels in mice supplemented with the dextran, compared to control (5% and 17.2%, respectively). Additionally, blood glucose levels decreased by 16.26% following dextran supplementation, while increasing by 15.2% in non-treated mice. Overall, this study displays biotherapeutic potential of a novel EPS to improve metabolic syndrome and its individual components, warranting further investigation.

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Section: Methodsmentioning

confidence: 99%

High-Molecular-Weight Dextran-Type Exopolysaccharide Produced by the Novel Apilactobacillus waqarii Improves Metabolic Syndrome: In Vitro and In Vivo Analyses

Ahmad

Boyajian

Abosalha

et al. 2022

IJMS

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“…The soluble sugars were analyzed using the method of Ahmad et al (2022) and Lee et al (2022) with some modifications. Briefly, 0.15 g of crushed soybean powder and 1.5 mL of deionized water were added to a 2 mL centrifuge tube, which was placed in a constant temperature shaker at 37 °C for 2 h at 180 r/min and then centrifuged at 12,000 r/min for 10 mins.…”

Section: Determination Of the Contents Of Soluble Sugars In Soybeanmentioning

confidence: 99%

Correlation analysis between soluble sugars of soybean and mucus drawing length of fermented natto

et al. 2022

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Natto is a traditional fermented food from Japan that is made from soybean fermented by Bacillus subtilis natto. The mucus drawing length of natto determines the sensory quality of natto, and there are differences in the mucus drawing length and sensory quality of natto fermented with soybeans with different contents of soluble sugars. In this study, soybeans with different contents of soluble sugars were used to ferment natto, and the differences and correlations between the mucilage composition, the mucus drawing length and the sensory quality of natto were investigated. The contents of oligosaccharides and total sugars in soybean were significantly positively correlated with the mucus drawing length (R = 0.921, R = 0.956), and the correlation between the drawing length of mucilage and total sugars was stronger. Fermenting soybeans with high contents of total sugars could make mucus drawing of natto longer. The contents of oligosaccharides and total sugars in soybean were significantly positively correlated with the sensory index score (R = 0.886, R = 0.925). The natto fermented from soybean with high contents of soluble total sugars had better overall sensory quality. Soluble sugars had a positive influence on mucus drawing length and sensory quality of natto.

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“…Additionally, fructans have many potential applications in cosmetics, medicine, industry, and biotechnology, including wound healing and immunostimulating activity [ 24 , 25 , 26 ]. For example, in addition to having a potential impact during sugar crop processing, fructan polysaccharides have been purposed for numerous biotechnological and medical uses, including immunomodulation [ 27 ], the pro-apoptotic treatment of neuroblastoma cells [ 5 , 28 ], and as an influence on digestion and gut microbiota as a prebiotic [ 5 , 25 , 29 ]. Therefore, there is ongoing interest in identifying various microbial levansucrases and the resulting polysaccharides for the development of various bio-based products and applications, since different levansucrases produce levans with a diverse range of sizes and branching [ 8 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

Bruni,

Qi,

Terrell

et al. 2024

Microorganisms

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During raw sugarcane processing, a significant portion of lost sucrose is attributable to microbial degradation. Sucrose consumption by many bacteria is also linked to the production of exopolysaccharides (EPS) such as dextrans and fructans. These resulting EPS cause operational challenges during raw sugar manufacturing. Here, we report the characterization of EPS from a fructan-forming Gluconobacter japonicus bacterium that we previously isolated from a Louisiana sugarcane factory. The genome sequencing revealed the presence of two encoded levansucrase genes, lsrA and lsrB. One levansucrase, LsrB, was detected in the secreted protein fraction of G. japonicus LASM12 by QTOF LC-MS. The spotting assays indicated that G. japonicus produces EPS using sucrose and raffinose as substrates. The G. japonicus EPS correlated with levan fructan commercial standards by 1H-NMR, and with the characteristic carbohydrate fingerprint region for FTIR spectra, confirming that the G. japonicus EPS is levan fructan. The glycosyl composition and glycosyl linkage analysis revealed a linear β-2,6-fructofuranosyl polysaccharide with occasional (5.7%) β-2,1-fructofuranosyl branches. The gel permeation chromatography of the levan fructan EPS showed two main peaks at 4.5 kDa and 8 kDa and a very minor peak at 500 kDa. G. japonicus was identified as a producer of levan fructan. These findings will be useful for future studies aimed at evaluating the impact of levan fructans on sugar crop processing, which have been historically underestimated in industry.

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Production of bimodal molecular weight levan by a Lactobacillus reuteri isolate from fish gut

Cited by 15 publications

References 56 publications

High-Molecular-Weight Dextran-Type Exopolysaccharide Produced by the Novel Apilactobacillus waqarii Improves Metabolic Syndrome: In Vitro and In Vivo Analyses

High-Molecular-Weight Dextran-Type Exopolysaccharide Produced by the Novel Apilactobacillus waqarii Improves Metabolic Syndrome: In Vitro and In Vivo Analyses

Correlation analysis between soluble sugars of soybean and mucus drawing length of fermented natto

Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

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