Production of bio-fuels (hydrogen and lipids) through a photofermentation process

Carlozzi, Pietro; Buccioni, Arianna; Minieri, Sara; Pushparaj, Benjamin; Piccardi, Raffaella; Ena, Alba; Pintucci, Cristina

doi:10.1016/j.biortech.2009.12.049

Cited by 39 publications

(25 citation statements)

References 31 publications

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“…Consequently, enhancement of the CBB cycle or other regulatory systems would be expected to increase CO 2 assimilation and hence increase organic carbon levels, leading to higher biomass production [30], [31]. To elucidate the effects of different CBB proteins on autotrophic growth, we investigated the differences in the growth of each manipulated strain by examining their biomass as dry cell weight (DCW) and constructed growth curves under photoautotrophic conditions.…”

Section: Resultsmentioning

confidence: 99%

Revealing the Functions of the Transketolase Enzyme Isoforms in Rhodopseudomonas palustris Using a Systems Biology Approach

Chang

Chen

et al. 2011

PLoS ONE

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Background Rhodopseudomonas palustris (R. palustris) is a purple non-sulfur anoxygenic phototrophic bacterium that belongs to the class of proteobacteria. It is capable of absorbing atmospheric carbon dioxide and converting it to biomass via the process of photosynthesis and the Calvin–Benson–Bassham (CBB) cycle. Transketolase is a key enzyme involved in the CBB cycle. Here, we reveal the functions of transketolase isoforms I and II in R. palustris using a systems biology approach.Methodology/Principal FindingsBy measuring growth ability, we found that transketolase could enhance the autotrophic growth and biomass production of R. palustris. Microarray and real-time quantitative PCR revealed that transketolase isoforms I and II were involved in different carbon metabolic pathways. In addition, immunogold staining demonstrated that the two transketolase isoforms had different spatial localizations: transketolase I was primarily associated with the intracytoplasmic membrane (ICM) but transketolase II was mostly distributed in the cytoplasm. Comparative proteomic analysis and network construction of transketolase over-expression and negative control (NC) strains revealed that protein folding, transcriptional regulation, amino acid transport and CBB cycle-associated carbon metabolism were enriched in the transketolase I over-expressed strain. In contrast, ATP synthesis, carbohydrate transport, glycolysis-associated carbon metabolism and CBB cycle-associated carbon metabolism were enriched in the transketolase II over-expressed strain. Furthermore, ATP synthesis assays showed a significant increase in ATP synthesis in the transketolase II over-expressed strain. A PEPCK activity assay showed that PEPCK activity was higher in transketolase over-expressed strains than in the negative control strain.Conclusions/SignificanceTaken together, our results indicate that the two isoforms of transketolase in R. palustris could affect photoautotrophic growth through both common and divergent metabolic mechanisms.

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Section: Resultsmentioning

confidence: 99%

Revealing the Functions of the Transketolase Enzyme Isoforms in Rhodopseudomonas palustris Using a Systems Biology Approach

Chang

Chen

et al. 2011

PLoS ONE

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“…Microalgae can use sunlight more efficiently than other crop plants to produce oil [168]. The oil production capacity is almost one or two times higher than any other crop [169].…”

Section: Raceway Pondmentioning

confidence: 99%

An overview on biofuel and biochemical production by photosynthetic microorganisms with understanding of the metabolism and by metabolic engineering together with efficient cultivation and downstream processing

Sarkar

Shimizu

2015

Bioresour. Bioprocess.

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Biofuel and biochemical production by photosynthetic microorganisms such as cyanobacteria and algae is attractive to improve energy security and to reduce CO 2 emission, contributing to the environmental problems such as global warming. Although biofuel production by photosynthetic microorganisms is called as the third generation biofuels, and significant innovation is necessary for the feasibility in practice, these fuels are attractive due to renewable and potentially carbon neutral resources. Moreover, photosynthetic microorganisms are attractive since they can grow on non-arable land and utilize saline and wastewater streams. Highly versatile and genetically tractable photosynthetic microorganisms need to capture solar energy and convert atmospheric and waste CO 2 to high-energy chemical products. Understanding of the metabolism and the efficient metabolic engineering of the photosynthetic organisms together with cultivation and separation processes as well as increased CO 2 assimilation enables the enhancement of the feasibility of biofuel and biochemical production.

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“…Biological production of hydrogen by industry involves (1) the biophotolysis of water with thermotolerant algae and cyanobacteria, (2) photosynthetic bacteria for the photofermentation of organic substances, and (3) "dark" fermentation mediated by anaerobic organisms, as shown in Figure 9.3 (Booth, 2005;Carlozzi et al, 2010;Wang et al, 2011). The latter mechanism supports a theoretical maximum yield of four molecules of H 2 per molecule of glucose, possible only with low H 2 partial pressure (p H2 ) (Lee et al, 2011;Nanqi et al, 2011).…”

Section: Thermophiles and Gaseous Fuels: Methane And Hydrogenmentioning

confidence: 99%

Extremophiles and their Application to Biofuel Research

Taylor¹,

Bauer²,

Mackay³

et al. 2012

Extremophiles

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Production of bio-fuels (hydrogen and lipids) through a photofermentation process

Cited by 39 publications

References 31 publications

Revealing the Functions of the Transketolase Enzyme Isoforms in Rhodopseudomonas palustris Using a Systems Biology Approach

Revealing the Functions of the Transketolase Enzyme Isoforms in Rhodopseudomonas palustris Using a Systems Biology Approach

An overview on biofuel and biochemical production by photosynthetic microorganisms with understanding of the metabolism and by metabolic engineering together with efficient cultivation and downstream processing

Extremophiles and their Application to Biofuel Research

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