Production of bioconcrete with improved durability properties using Alkaliphilic Egyptian bacteria

Ahmed, Shiren O.; Nasser, Amal A.; Abbas, Rateb; Kamal, Monir M.; Zahran, M. A.; Sorour, Noha M.

doi:10.1007/s13205-021-02781-0

Cited by 21 publications

(41 citation statements)

References 40 publications

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“…Bacillus megaterium was originally isolated from the alkaline soil samples located at Wadi EL-Nitron, Behera governorate, Egypt. It was identified using MALDI-TOF Biotyper (Matrix-Assisted Laser Desorption Ionization Time-Of-Flight, Bruker) identifying the ribosomal proteins using mass spectrometry [16]. B. megaterium has a rod-shaped, gram positive; endospore forming species of bacteria having doubling time of about 1.6 h is chosen for the investigation.…”

Section: Bacterial Strain and Growth Conditionsmentioning

confidence: 99%

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Effect of Bacillus Megaterium Bacteria and Different Calcium Source on Strength and Permeation Properties of Concrete

Nasser

Esmail

Abbas

et al. 2022

ERJ. Engineering Research Journal

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Different methods were used to repair concrete cracks. Recently, microbial induced calcium carbonate precipitation is presented as a self-healing technique for concrete manufacture, which is proposed as an eco-friendly method. It is a bio-mineralization process. Microbial activities induced precipitation that can heal cracks. Effect of bio-mineralization in concrete by incorporation of ureolytic species such as Bacillus Megaterium is investigated. Fresh and hardened tests were carried out on concrete at various ages. "Bacillus Megaterium" (Bm) with a cell density of 2× CFU/mL was used when added to mixtures with two ratios of 0.5% and 0.25% of cement weight. Calcium lactate, Calcium acetate, and Calcium format were added as nutrition to bacteria by 0.25% and 0.125% of cement weight. Test results indicated that the inclusion of Bm in concrete enhanced properties. Bacteria addition and all nutritions used revealed varied decreases in the rate of water absorption and capillary permeability coefficient. Bm 0.5% with acetate nutrition concrete mix had a maximum increase in compressive strength at all ages. It had compressive strength value 152.99% of that of the compressive strength of the control concrete mixture at 28days. Bm 0.5% with lactate nutrition concrete mix had flexural strength value 162.74% of that of control concrete flexural strength at 28days. Enhancement of properties was due to calcite deposition on the bacteria cell surfaces within the pores. SEM imaging indicated the formation of calcite crystals in different shapes according to the added nutrients of bacterial concrete specimens.

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Section: Bacterial Strain and Growth Conditionsmentioning

confidence: 99%

“…Rhombohedra morphology of calcite crystals was observed using calcium Formate as shown in figure (5.e, f). Those crystals have SEM imaging in many papers [15,16,28]. SEM image examination revealed that bacterial concrete matrix using a super plasticizer was more compact and dense.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Effect of Bacillus Megaterium Bacteria and Different Calcium Source on Strength and Permeation Properties of Concrete

Nasser

Esmail

Abbas

et al. 2022

ERJ. Engineering Research Journal

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“…Concrete is the most widely used construction material, with over 6 million m 3 produced worldwide every year, because of its high compressive strength, casting, and relatively low cost [ 1 , 2 ]. It does, however, has limited tensile strength, and diminutive resistance to cracking.…”

Section: Introductionmentioning

confidence: 99%

“…Developing bio-based self-healing concrete aims to reduce cracking-related durability issues. Therefore, researchers are trying to exploit self-healing approaches [ 2 , 4 , 5 , 7 , 8 , 9 , 10 , 11 ]. Novel biomaterials, based on the enhancement of calcite-precipitation have been developed for self-healing of cracks [ 5 , 7 , 8 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…The addition of selected microorganisms into cementitious materials is considered cost-effective and eco-friendly method for micro-cracks repair. Microbial self-healing is a very promising technique, because it is a continuous process for healing of micro-cracks, leading to substantial improvement in the physico-mechanical properties of bioConcrete [ 2 , 4 , 5 , 12 , 14 ]. Therefore, microbial self-healing can increase the service life of concrete structures without using costly and/or time consuming interventions [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Microbially-Induced-Calcite-Precipitation (MICP): A biotechnological approach to enhance the durability of concrete using Bacillus pasteurii and Bacillus sphaericus

Nasser

Sorour

Saafan

et al. 2022

Heliyon

Self Cite

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Impact of bacterial admixtures on the compressive and tensile strengths, permeability, and pore structure of ternary mortars: Comparative study of ureolytic and phototrophic bacteria

Aceituno‐Caicedo,

Shvarzman,

Zhutovsky

et al. 2023

Biotechnology Journal

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The addition of bacterial biomass to cementitious materials can improve strength and permeability properties by altering the pore structure. Photoautotrophic bacteria are understudied mortar bio‐additives that do not produce unwanted by‐products compared to commonly studied ureolytic species. This study directly compares the impact of the addition of heterotrophic Bacillus subtilis to photoautotrophic Synechocystis sp. PCC6803 on mortar properties and microstructure. Cellulose fibers were used as a bacteria carrier. A commercial concrete healing agent composed of dormant bacterial spores was also tested. Strength, water absorption tests, mercury intrusion porosimetry, differential scanning calorimetry, thermogravimetric analysis, and scanning electron microscopy were applied to experimental mortar properties. The photoautotrophic modifications had a stronger positive impact on mortar strength and permeability properties than sporulated heterotrophic modifications due to differences in surface properties and production of exopolysaccharides. The findings provide support for photoautotrophic species as additives for mortars to move away from ammonia‐generating species.

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Production of bioconcrete with improved durability properties using Alkaliphilic Egyptian bacteria

Cited by 21 publications

References 40 publications

Effect of Bacillus Megaterium Bacteria and Different Calcium Source on Strength and Permeation Properties of Concrete

Effect of Bacillus Megaterium Bacteria and Different Calcium Source on Strength and Permeation Properties of Concrete

Microbially-Induced-Calcite-Precipitation (MICP): A biotechnological approach to enhance the durability of concrete using Bacillus pasteurii and Bacillus sphaericus

Impact of bacterial admixtures on the compressive and tensile strengths, permeability, and pore structure of ternary mortars: Comparative study of ureolytic and phototrophic bacteria

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