Production of Biodiesel Feedstock - Microbial Lipid From Slaughterhouse Wastewater

Asokan, Thirulogachandar; Singaram, Jayanthi; Sundaram, Priyadharshni Vairava

doi:10.1504/ijesd.2018.10013118

Cited by 1 publication

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“…The samples were filtered using Whatman No. 1 filter paper, and the carbon nitrogen (C/N) ratio was calculated using a TOC analyzer 26 …”

Section: Methodsmentioning

confidence: 99%

“…In the flask containing pretreated wastewater, 2% inoculum was added. The flasks were kept for 5 days at 30°C and 120 rpm on an orbital shaker 26 …”

Section: Methodsmentioning

confidence: 99%

“…The flasks were kept for 5 days at 30 C and 120 rpm on an orbital shaker. 26 A 5 L autoclavable bio-age fermenter (Bio-Spin 05A, Bio-Age, India) was employed for mass biomass cultivation, and the bioprocess was monitored for pH, DO, and stirring. To keep the temperature stable, a condenser configuration was employed.…”

Section: Microbial Strain Cultivationmentioning

confidence: 99%

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Production of sustainable biodiesel from alkaline pretreated dairy effluent using Yarrowia lipolytica

Srirajarajeshwari,

Jeyanthi

2023

Env Prog and Sustain Energy

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Biodiesel (BD) has grown in popularity over the last few years due to the global energy crisis, in addition to the depletion of reserves and the shortage of oil. The current study investigates the utilization of dairy effluent as a substratum for Yarrowia lipolytica, an oily yeast employed in biofuel generation. The dairy effluent has high biochemical oxygen demand, dissolved solids and, low pH. In this study, based on carbon nitrogen ratio (C/N), the alkaline pretreatment method (KOH 5%) was adopted to grow Y. lipolytica in dairy effluent. After 5 days of growth at 29°C, pH 6.9 (initial pH of dairy effluent), and stirring speed 120 rpm, the growth of Y. lipolytica produced 42.3 g/L biomass. Utilizing an ex‐situ transesterification method, the biomass was metamorphosed into BD. Using a modified version of Folch, Bligh, and Dyer's approach, lipid removal was done as the initial step. Moreover, the lipid was transesterified in a KOH catalyst, yielding 61% BD. Utilizing Fourier transform infrared spectroscopy analysis, the produced fatty acid methyl ester was examined.

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“…The samples were filtered using Whatman No. 1 filter paper, and the carbon nitrogen (C/N) ratio was calculated using a TOC analyzer 26 …”

Section: Methodsmentioning

confidence: 99%

“…In the flask containing pretreated wastewater, 2% inoculum was added. The flasks were kept for 5 days at 30°C and 120 rpm on an orbital shaker 26 …”

Section: Methodsmentioning

confidence: 99%