Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids

Efficient biosynthesis of γ-decalactone in ionic liquids by immobilized whole cells of Yarrowia lipolytica G3-3.21 on attapulgite

“…The operational stability of immobilized Y. lipolytica G3-3.21 cells was examined in ionic liquids/buffer biphasic system and Tris-HCl buffer system as described by Arai et al [18] with some modifications. Aliquots of the immobilized cells were added into the corresponding mixture, which are Tris-HCl buffer system: 30 mL of Tris-HCl buffer (50 mM, pH 7.5) containing 20 % of glucose, 5 % of castor oil and 0.05 % of CaCl 2 ; 30 mL of ionic liquids/buffer biphasic system containing 2 mL of ionic liquids and 28 mL Tris-HCl buffer (50 mM, pH 7.5) containing 20 % of glucose, 5 % of castor oil and 0.05 % of CaCl 2 .…”

Application of Ionic Liquids in Biocatalysis

“…) biphasic systems following a 24 h reaction (Arai et al, 2010). Numerous types of enzymatic reactions (Habulin et al, 2007b;Contesini & Carvalho, 2006;Lou et al, 2006;Tan et al, 2007;Hernandez-Fernandez et al, 2007) have been carried out using ILs as solvents with similar or enhanced reaction rates and enzyme activities, and with higher operational stabilities and enantioselectivities compared to those observed in organic solvents (Welton, 1999;van Rantwijk et al, 2003;Kragl et al, 2002).…”

Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route

“…Though the relationship of cell morphology and enzyme secretion depends on fungal strain and enzyme type, cell immobilization inducing pellet formation strongly inhibit R. oryzae lipase, particularly from the membrane. Moreover, immersed biocatalyst in glutaraldehyde solution induces intra-and inter-molecular cross-linking of lipase [11]. BSPs.…”

“…Utilizing whole-cell overproducing intracellular lipase, such as Rhizopus oryzae, as biocatalyst in biodiesel synthesis is a potential way to reduce the biocatalyst cost [10]. The preparation of a biocatalyst requires no complex purification process, and the prepared whole-cells could be directly used as lipase containers [11].…”