Production of biodiesel via ethanolysis of waste cooking oil using immobilised lipase

Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases

“…The transesterification of vegetable oils and animal fats using short-chain alcohols (methanol and ethanol) to produce alkyl esters to be used, as biodiesel fuel has been extensively researched [1][2][3]. In industrial scale, biodiesel is mainly produced following the conventional chemical process where basic catalysts are used, which is efficient in terms of reaction time and yields.…”

Kinetic Study for the Ethanolysis of Fish Oil Catalyzed by Lipozyme^® 435 in Different Reaction Media

“…Not much data of activation energy for ethanolysis reaction of oil by lipases has been reported in the literature. Chesterfield et al 9 reported a value for the activation energy of 17.3 kJ/mol for the global reaction rate of ethanolysis of waste cooking oil to fatty acid ethyl esters in the temperature range between 297 to 320 K, catalyzed by Novozym 435.…”

“…The most commonly used mechanism to describe the alcoholysis of triglycerides is the Ping-Pong Bi-Bi mechanism 16 . Alcohol and substrate inhibition of enzyme are often considered 9 . In many of these studies only initial changes of substrates were considered to estimate the kinetic parameters of the model and formation of di-and monoglycerides are not considered 17 .…”

“…1 . Lipase-catalyzed ethanolysis of triglycerides takes places yielding one molecule of fatty acid ethyl ester and a glyceride containing one fewer ester bond at each step, that is DAG, MAG and glycerol as last product 9 :…”

“…However, the Candida antarctica lipase B used in this work has been described to be still active at very low water content in the system 8 . In addition, it has also a high resistance to alcohol deactivation 9 .…”