“…Cellulose quality, temperature, aeration, carbon sources, incubation period, medium additives pH of the medium and presence of inducers are important parameters for the optimized production of cellulase enzymes. Other researchers have also reported cellulase production enhancement by the optimization of cultural conditions [28][29][30][31][32]. Thus, in the present study, quantitative estimation and optimization of the production of bioethanol with appropriate substrate, i.e, leaf, are required for the pilot scale and large-scale business.…”