Production of biofilm by Listeria monocytogenes in different materials and temperatures

Bonsaglia, Erika Carolina Romão; Silva, N.C.C.; Júnior, Araújo; Júnior, João Pessoa Araújo; Tsunemi, Mirian Harumi; Rall, Vera Lúcia Mores

doi:10.1016/j.foodcont.2013.07.023

Cited by 141 publications

(97 citation statements)

References 39 publications

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“…Several studies (Bonsaglia et al, 2014;Chae et al, 2000;Gião and Keevil, 2014;Mai and Conner, 2007;Min et al, 2006) have demonstrated the ability of several strains of L. monocytogenes to form biofilms at different temperatures, nutrient conditions and on different surfaces. Strongly adherent L. monocytogenes strains were found to be more…”

Section: Introductionmentioning

confidence: 99%

Differential internalin A levels in biofilms of Listeria monocytogenes grown on different surfaces and nutrient conditions

Gilmartin

Gião

Keevil

et al. 2016

International Journal of Food Microbiology

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Listeria monoctyogenes is a foodborne pathogen containing the surface protein, internalin A (InlA). The expression of this protein permits the invasion of L. monocytogenes into intestinal epithelial cells expressing the receptor E-cadherin, thus crossing the intestinal barrier and resulting in listerosis. The main aim of this work was to investigate InlA levels in different L. monocytogenes strains in both planktonic and sessile states using an anti-InlA antibody. Biofilms were grown in high and low nutrient environments on glass, stainless steel and polytetrafluoroethylene (PTFE). This study demonstrated that InlA levels varied greatly between strains and serotypes of L. monocytogenes. However, the serotypes 1/2a, 1/2b and 4b, associated with the largest number of outbreaks of listerosis consistently showed the highest InlA levels, regardless of nutrient content or planktonic or sessile state.Differences in InlA levels were also observed in biofilms grown on different surfaces such as glass, stainless steel and PTFE, with a significant reduction in InlA levels observed in biofilms on PTFE. Interestingly, although a large number of the total cells observed in biofilms formed in tap-water were non-cultivable, the virulence factor, InlA, was expressed at levels between 78-85%, thus indicating that these cells may still be virulent. A greater understanding of the factors that affect the levels of InlA on the surface of L. monocytogenes, is essential in the appreciation of the role of InlA in the persistence of biofilms containing L. monocytogenes and their potential to cause food borne disease.  InlA levels in biofilms were not affected by low nutrient conditions but affected by growth on different surfaces such as glass, stainless steel and polytetrafluoroethylene. Key words

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Section: Introductionmentioning

confidence: 99%

Differential internalin A levels in biofilms of Listeria monocytogenes grown on different surfaces and nutrient conditions

Gilmartin

Gião

Keevil

et al. 2016

International Journal of Food Microbiology

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“…SS, Cu and GLA were selected because of their common use in heat exchange equipment and pipes in food processing lines (Bonsaglia et al, 2014;Brooks and Flint, 2008;Melo and Flemming, 2010;Shi and Zhu, 2009;Van Houdt and Michiels, 2010).…”

Section: Surface Preparationmentioning

confidence: 99%

“…(Van Houdt and Michiels, 2010). Stainless steel, glass and copper have been intensively used in food industries (Bonsaglia et al, 2014;Brooks and Flint, 2008;Melo and Flemming, 2010;Shi and Zhu, 2009;Van Houdt and Michiels, 2010). Stainless steel tubing is often used because of its hygienic status (low soiling level and/or high cleanability) (Jullien et al, 2003) and the ability to resist corrosive damage (Flint et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

The impact of material properties, nutrient load and shear stress on biofouling in food industries

Moreira

Gomes

Simões

et al. 2015

Food and Bioproducts Processing

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Abstract:In the food industry, biofilm formation in pipes, equipment and cooling systems increases maintenance costs, decreases operational efficiencies and is a source of contamination.Shear stress, nutrient load and surface material are important variables affecting the biofilm onset in industry. In this work, the combined impacts of these variables were assessed using three different materials (glass, copper and stainless steel), two nutrient loads (high and low nutrient medium) and two hydrodynamic conditions (static and dynamic). Initial adhesion and biofilm formation were studied in microplates usingEscherichia coli as a model organism.Surface material was the factor with the strongest impact and adhesion/biofilm formation were correlated with surface hydrophobicity. However, the impact of this variable was dependent on the nutrient load and imposed shear stress. It was also found that, for the majority of the situations tested, initial attachment performance is a good predictor of biofilm formation behaviour and that the effects observed during attachment are amplified during biofilm maturation. Since shear stress is a major determinant in cell adhesion, the results of this study may find application in industrial systems operating at flow rates between 0.001 and 600 m 3 h -1 depending on tube material and diameter.

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“…Indeed, the ability of pathogenic bacteria, including S. enterica, to form biofilms on various surfaces and under different environmental conditions has been investigated in several recent research studies (Bonsaglia et al, 2014;Díez-García et al, 2012;Lianou and Koutsoumanis, 2012;Nilsson et al, 2011). However, despite the widespread application of mathematical models for the prediction of microbial growth and survival, and the significant amount of scientific literature on modelling of biofilm processes (Beg and Chaudhry, 1999;Wanner et al, 2006), there has not been made any attempt until now to develop and implement mathematical models for the description of the effects of environmental factors on biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Modelling biofilm formation of Salmonella enterica ser. Newport as a function of pH and water activity

Dimakopoulou-Papazoglou

Nychas

Koutsoumanis

2016

Food Microbiology

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The effect of pH and water activity (aw) on the formation of biofilm by Salmonella enterica ser. Newport, previously identified as a strong biofilm producer, was assessed. Biofilm formation was evaluated in tryptone soy broth at 37 °C and at different combinations of pH (3.3-7.8) and aw (0.894-0.997). In total, 540 biofilm formation tests in 108 pH and aw combinations were carried out in polystyrene microtiter plates using crystal violet staining and optical density (OD; 580 nm) measurements. Since the individual effects of pH and aw on biofilm formation had a similar pattern to that observed for microbial growth rate, cardinal parameter models (CPMs) were used to describe these effects. CPMs described successfully the effects of these two environmental parameters, with the estimated cardinal values of pHmin, pHopt, pHmax, awmin and awopt being 3.58, 6.02, 9.71, 0.894 and 0.994, respectively. The CPMs assumption of the multiplicative inhibitory effect of environmental factors was validated in the case of biofilm formation using additional independent data (i.e. 430 OD data at 86 different combinations of pH and aw). The validation results showed a good agreement (r(2) = 0.938) between observed and predicted OD with no systematic error. In the second part of this study, a probabilistic model predicting the pathogen's biofilm formation boundaries was developed, and the degree of agreement between predicted probabilities and observations was as high as 99.8%. Hence, the effect of environmental parameters on biofilm formation can be quantitatively expressed using mathematical models, with the latter models, in turn, providing useful information for biofilm control in food industry environments.

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Production of biofilm by Listeria monocytogenes in different materials and temperatures

Cited by 141 publications

References 39 publications

Differential internalin A levels in biofilms of Listeria monocytogenes grown on different surfaces and nutrient conditions

Differential internalin A levels in biofilms of Listeria monocytogenes grown on different surfaces and nutrient conditions

The impact of material properties, nutrient load and shear stress on biofouling in food industries

Modelling biofilm formation of Salmonella enterica ser. Newport as a function of pH and water activity

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