Production of biosurfactant by a Pseudomonas aeruginosa isolate and its applicability to in situ microbial enhanced oil recovery under anoxic conditions

Zhao, Feng; Zhang, Jie; Shi, Ronghua; Han, Shun; Ma, Fang; Zhang, Ying

doi:10.1039/c5ra03559g

Cited by 50 publications

(53 citation statements)

References 32 publications

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“…They aimed at a comprehensive characterization of the material and at unravelling how the rhamnolipid anions are introduced into the interlayers of LDH. ATR-IR spectroscopy for the qualitative identification of rhamnolipids produced in fermentation processes has been reported as well [13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Infrared Spectroscopy for Studying Structure and Aging Effects in Rhamnolipid Biosurfactants

2017

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Biosurfactants are produced by microorganisms and represent amphiphilic compounds with polar and non-polar moieties; hence they can be used to stabilize emulsions, e.g., in the cosmetic and food sectors. Their structure and its changes when exposed to light and elevated temperature are yet to be fully understood. In this study, we demonstrate that attenuated total reflection infrared (ATR-IR) spectroscopy is a useful tool for the analysis of biosurfactants, using rhamnolipids produced by fermentation as an example. A key feature is that the analytical method does not require sample preparation despite the high viscosity of the purified natural product.

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Section: Introductionmentioning

confidence: 99%

Infrared Spectroscopy for Studying Structure and Aging Effects in Rhamnolipid Biosurfactants

2017

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“…Rhamnolipid with excellent properties such as biodegradability, low toxicity, antimicrobial, good surface activity and emulsi cation [1,2] and usually produced by Pseudomonas aeruginosa [3], was currently the most potential biosurfactant to apply in oil recycling [4,5] and environmental protection [6], food processing [7,8] and biomedical industry [9,10]. However, despite their potential, the industrial application of rhamnolipid was restricted seriously since the cost of rhamnolipid production was approximate ten times that of synthetic surfactant [11].…”

Section: Introductionmentioning

confidence: 99%

Enhancing rhamnolipid production and exploring the mechanisms of low-foaming fermentation under weak-acid conditions

Gong

Yang

Che

et al. 2020

Preprint

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The commercialized applications of rhamnolipid has seriously impeded by high cost of production caused by severe foaming. Effective low-foaming fermentation and mechanism study have become the most urgent requirement for larger-scale production of rhamnolipid. In this study, the low-foaming fermentation was realized by controlling fermentation at pH 5.5. Further, the production of rhamnolipid was enhanced 0.9-fold by the ultraviolet and ethyl methanesulfonate composite mutagenesis. To the best of our knowledge, this was the first report to enhance production of rhamnolipid by strain improvement at weak-acid conditions. The mechanisms exploration tests indicated that increasing surface tension and decreasing viscosity were conducive to reduce foaming ability of rhamnolipid fermentation. The decrease of negatively charged ions may weaken the electrostatic repulsion between charged substances adsorbed membranes of bubbles such as rhamnolipid and cells, leading to the liquid membranes to rupture easily. In addition, the structure of vesicle or lamellar of rhamnolipid formed at pH5.0-6.0 may also weaken the foaming ability of rhamnolipid fermentation. The work revealed the promising potentiality for genetic modification to enhance the production of rhamnolipid and facilitated the understanding of pH-associated foaming behavior, as well as were conducive to develop a more effective pH-associated control strategies for large-scale production of rhamnolipid.

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“…The anaerobic biosynthesis of rhamnolipids has important research and practical signi cance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants [8]. Aerobic production of rhamnolipids fails to meet the in situ applications in the anoxic environments, such as deep soil, oil reservoirs, sediments [8][9][10]. The foam problem in biosurfactant fermentation has been perplexing the production of biosurfactants [11].…”

Section: Introductionmentioning

confidence: 99%

“…Although the mainly rhamnolipids producers, Pseudomonas aeruginosa, are facultative bacteria [8,13,14], only few studies have reported the anaerobic biosynthesis of rhamnolipids by rare P. aeruginosa strains [9,10,15,16]. P. aeruginosa can grow and metabolize at both aerobic and anaerobic conditions.…”

Section: Introductionmentioning

confidence: 99%

“…In previous studies, using facultative anaerobic and denitrifying bacterial strain P. stutzeri DQ1, the constructed engineering bacterial strain P. stutzeri Rhl achieved heterologous production of P. aeruginosa rhamnolipids under anaerobic conditions [18]. A wild-type P. aeruginosa SG that can anaerobically produce rhamnolipids was subsequently screened from production water of Xinjiang oil reservoir [9]. During the process of medium optimization, it was found that strain P. aeruginosa SG could anaerobically grew well using glucose and glycerol, but strain SG anaerobically produced rhamnolipids using glycerol not glucose [19].…”

Section: Introductionmentioning

confidence: 99%

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Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

Zhao

Guo

Cui

2020

Preprint

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Background: Rhamnolipids is the most widely studied and applied biosurfactants. The anaerobic biosynthesis of rhamnolipids has important research and practical significance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants. A few studies have reported the anaerobic biosynthesis of rhamnolipids from rare Pseudomonas aeruginosa strains. What did promote the anaerobic biosynthesis of rhamnolipids, the specificity of the rare strains or the effect of specific substrates? Here, anaerobic production of rhamnolipids by different P. aeruginosa strains was investigated using diverse substrates. The anaerobic biosynthesis mechanism of rhamnolipids were also discussed from the substrate point of view.Results: All P. aeruginosa strains anaerobically grew well using the tested substrates. But all P. aeruginosa strains anaerobically produced rhamnolipids only using substrates containing glycerol and nitrate. Fourier transform infrared (FTIR) spectra analysis confirmed the anaerobic production of rhamnolipids from all P. aeruginosa strains. All the anaerobically produced rhamnolipids decreased air-water surface tension from 72.6 mN/m to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Using crude glycerol as low-cost substrate, all P. aeruginosa strains can anaerobically grow and produce rhamnolipids to reduce the culture surface tension below 35 mN/m. The glycerol metabolic intermediate, 1, 2-propylene glycol, can also achieve the anaerobic production of rhamnolipids by all P. aeruginosa strains.Conclusions: Not the specificity of the rare P. aeruginosa strains but the effect of specific substrates promote the anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Glycerol and nitrate are the excellent substrates for anaerobic production of rhamnolipids from all P. aeruginosa strains. Results indicated that glycerol metabolism involveed the anaerobic biosynthesis of rhamnolipids in P. aeruginosa. Results also showed the feasibility of using crude glycerol as low cost substrate to anaerobically biosynthesize rhamnolipids by P. aeruginosa.

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Production of biosurfactant by a Pseudomonas aeruginosa isolate and its applicability to in situ microbial enhanced oil recovery under anoxic conditions

Abstract: An isolated strain SG can produce biosurfactant under anoxic conditions and has great potential for in situ microbial enhanced oil recovery.

Cited by 50 publications

References 32 publications

Infrared Spectroscopy for Studying Structure and Aging Effects in Rhamnolipid Biosurfactants

Infrared Spectroscopy for Studying Structure and Aging Effects in Rhamnolipid Biosurfactants

Enhancing rhamnolipid production and exploring the mechanisms of low-foaming fermentation under weak-acid conditions

Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

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