Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

Abstract

BackgroundThe study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish.Material and MethodsAn in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours.ResultsA statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group).ConclusionsIn comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it. Key words:BMP-2, Cytoskeleton, cell culture, bone matrix, apoptosis, cell viability.

Effect of bone morphogenetic protein 9 on osteoblast differentiation of cells grown on titanium with nanotopography

“…For this study BMP-9 was chosen due to its higher osteogenic potential compared with other BMPs, such as BMP-2, -4, and -7. 33,34 Considering that osteoblasts express some BMPs with osseoinductive capacity like BMP-2, -4 and -7, [35][36][37] we evaluated the expression of endogenous BMP-9 in both MC3T3-E1 cells and osteoblasts differentiated from bone marrow mesenchymal stem cells in order to investigate FIGURE 3 Gene expression of the osteoblast markers RUNX2, OSX, OC, BSP in MC3T3-E1 cells grown on Ti-Nano (A) or Ti-Machined (B) and treated with 20 nM of BMP-9 or vehicle (Control) on Day 7. The data presented are mean ± standard deviation (n = 3) and different letters indicate a statistically significant difference between Control and BMP-9 for each evaluated gene (P ≤ 0.05) its role in osteoblast differentiation induced by Ti-Nano.…”

“…For this study BMP-9 was chosen due to its higher osteogenic potential compared with other BMPs, such as BMP-2, -4, and -7. 33,34 Considering that osteoblasts express some BMPs with osseoinductive capacity like BMP-2, -4 and -7, [35][36][37] we evaluated the expression of endogenous BMP-9 in both MC3T3-E1 cells and osteoblasts differentiated from bone marrow mesenchymal stem cells in order to investigate its role in osteoblast differentiation induced by Ti-Nano. However, BMP-9 gene expression was not detected in these cells, unlike organs and tissues where this protein is known to be expressed (Figure S1), 38,39 suggesting that despite the relevance of BMP-9 to osteoblast differentiation, this protein is not synthesized by bone tissue.…”