Production of carbon dioxide and lactic acid from radioactive glucose by tissue in culture

The plateau phase of growth of the L-M strain mouse cell in a protein-free medium

“…A burst of lactic acid production occurred after each addition of glucose. This result was in accord with the experiments of Broda et al [5] acid and protein was obtained from intact cells previously exposed to uniformly labelled l*C glucose and administered to L-M cells during logarithmic growth when cell viability and metabolic activity is optimal. and that re-utilization of large molecules released into the medium was possible.…”

CHANGES IN HeLa CELL ULTRASTRUCTURE UNDER CONDITIONS OF REDUCED GLUCOSE SUPPLY

“…Under I hese conditions, the cellular glycolysis as measured by lactic acid production was reduced to zero. In a radiometric study of chick fibroblast metabolism, Broda et al (1961) observed a decrease in the glycolysisirespiration ratio (corresponding to the Crabtree effect) as the cell number per vol~unc unit of medium is increased.…”