Production of carbonic anhydrase by marine and freshwater microalgae

Ores, Joana da Costa; Amarante, Marina Campos Assumpção de; Fernandes, Sibele Santos; Kalil, Susana Juliano

doi:10.1080/10242422.2016.1227793

Cited by 12 publications

(2 citation statements)

References 56 publications

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“…The activity of carbonic anhydrase (CA) was determined using the following procedure: moderate amounts of algal cells were harvested and suspended in 10 mM Tris-HCl buffer (pH 8.3) [28]. Then cells were disrupted by ultrasonic treatment and centrifuged to collect the supernatant.…”

Section: Carbonic Anhydrase and Rubisco Activity Assaymentioning

confidence: 99%

Enhanced biomass and CO2 sequestration of Chlorella vulgaris using a new mixotrophic cultivation method

Wang

Xiao

et al. 2020

Process Biochemistry

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CO 2 sequestration using microalgae has been deemed as a promising way, which can fix CO 2 and simultaneously produce valuable bio-products. However, large scale open-pond production of Chlorella vulgaris is normally cultured using acetic acid as a carbon source, which is costly and shows a low efficiency in CO 2 fixation. Here, a new mixotrophic culture strategy using both CO 2 and acetic acid was developed and evaluated for CO 2 sequestration by a C. vulgaris culture in an open pond. Our results show that the growth rate of C. vulgaris under this new mixotrophic condition reaches 0.24 g/L/d, which is higher than the 0.15 and 0.21 g/L/d of photoheterotrophic culture with acetic acids and photoautotrophic culture with CO 2 , respectively. The averaged CO 2 fixation rate was determined as 0.29 g/L/d, which is much higher than heterotrophic method but slightly lower than photoautotrophic method. This result was further confirmed in a 125 m 2 open raceway pond. Physiological and biochemical characterization showed that the cell quality of C. vulgaris under mixotrophic conditions is better than those of photoautotrophic method and photoheterotrophic cultures. The enzyme activity assay and transcriptome sequencing analysis revealed that the metabolism of carbohydrates and amino acids was significantly enhanced under mixotrophic condition compared with other groups, which may attribute to the increased biomass and CO 2 sequestration of C. vulgaris. Our results suggest that this mixotrophic strategy can be applied in large-scale cultivation of C. vulgaris for biomass production and CO 2 sequestration. Recently, C. vulgaris has become the second largest cultured algal in China [14], and it is normally cultured in open raceway ponds using acetic acid [15,16], where acetic acid act as a carbon source and also keeps the pH within neutral range [17]. Compared with acetic acid, CO 2 can also be used by C. vulgaris as carbon source with the advantages of higher CO 2 fixation efficiency, and lower-risk of contamination [18]. However, C. vulgaris cells cultured only with CO 2 are inferior to those with acetic acids or glucose in terms of cell size and density, and are difficult to harvest [19]. Therefore, improvement of biomass production and CO 2 sequestration rate is an area of great

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Section: Carbonic Anhydrase and Rubisco Activity Assaymentioning

confidence: 99%

Enhanced biomass and CO2 sequestration of Chlorella vulgaris using a new mixotrophic cultivation method

Wang

Xiao

et al. 2020

Process Biochemistry

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“…To supplement the medium, 0.1 mL of vitaminic solution and 1 mL of Conway medium were added (Walne 1979). The microalgae growth Cienc (2022) 94(4) e20201735 3 | 12 was promoted by continuous illumination with fluorescent lamps (40.5 µE m -2 s -1 ) with a 24-hour photoperiod and constant aeration through injection of atmospheric air (Ores et al 2016). The medium was maintained at room temperature (25°C).…”

Section: Microalgae and Culture Mediummentioning

confidence: 99%

Effect of the seed coating with biomass of Dunaliella salina on early plant growth and in the secondary metabolites content of Coriandrum sativum

Fonseca

Almeida

Caldas

et al. 2022

An. Acad. Bras. Ciênc.

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The environmental and health risks associated with the application of synthetic chemical inputs in agriculture increased the demand for technologies that allow higher performance and quality of vegetable crops by implementing synergistic materials with the principles of sustainability. In this work, the seed coating with the biomass of Dunaliella salina incorporated in a bioplastic fi lm of Manihot esculenta (cassava) was evaluated as an initial growth and secondary compounds stimulator of Coriandrum sativum (coriander) plants. The obtained results demonstrated that the coating stimulated an increase in the germination percentage (28.75%) and also in concentration of bioactive compounds, such as the six-fold increment of caffeic acid (13.33 mg 100 g -1 ). The carbohydrates, lipids, and proteins present in the microalgae biomass seem to be responsible for these increments once they are known for providing energy to the seedling development and coordinating the secondary metabolites synthesis. As conclusion, we consider the coating with biomass of D. salina an alternative for crop improvement that contributes to the development of sustainable agricultural practices.

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An Evidence of Carbonic Anhydrase Activity in Native Microalgae for CO2 Capture Application

Davoodbasha,

Kathiravan,

Jayakannan

et al. 2024

Appl Biochem Biotechnol

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Production of carbonic anhydrase by marine and freshwater microalgae

Cited by 12 publications

References 56 publications

Enhanced biomass and CO2 sequestration of Chlorella vulgaris using a new mixotrophic cultivation method

Enhanced biomass and CO2 sequestration of Chlorella vulgaris using a new mixotrophic cultivation method

Effect of the seed coating with biomass of Dunaliella salina on early plant growth and in the secondary metabolites content of Coriandrum sativum

An Evidence of Carbonic Anhydrase Activity in Native Microalgae for CO2 Capture Application

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