Production of cardio-active substances by plant tissue cultures and their screening for cardiovascular activity

Kaul, B. L.; Wells, Patrick H.; Staba, E. John

doi:10.1111/j.2042-7158.1967.tb08028.x

Cited by 19 publications

(4 citation statements)

References 5 publications

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“…Later chromatograms were exposed to I 2 vapours, marked, evaporated and these spots were scraped and the compounds eluted with chloroform and concentrated. Each of the compounds were crystallized with methanol and acetone (1:2; Kaul et al , 1967). The crystals were dried in vacuo and subjected to melting point and various physical and spectral studies for characterization (Heilbron and Bunbury, 1953;Yamaguchi, 1970).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial activity of terpenoids from Trichodesma amplexicaule Roth.

Singh

2003

Phytotherapy Research

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T. amplexicaule Roth. has been investigated phytochemically for biologically active constituents from the benzene extract. This yielded a mixture of terpenoids: beta-sitosterol, alpha-amyrin, lupeol, hexacosanoic acid, ceryl alcohol and hexacosane. The sequential extracts, isolated terpenoids and reference antibiotics were screened for their possible bioactivities against selected pathogenic bacteria, Escherichia coli, Staphylococcus aureus and fungi, Aspergillus flavus and Penicillium chrysogenum etc. Out of the sequential extracts, the benzene extract was more potent against S. aureus (IZ = 10.36) and R. phaseoli (IZ = 10.69). The inhibition zones of isolated terpenoids were also recorded and the activity index was calculated and it was found that hexacosane was more active (IZ = 13.39) against E. coli and hexacosanoic acid had greater activity against A. flavus (IZ = 11.56). The present study deals with antimicrobial screening of sequential extracts and isolated terpenoids.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial activity of terpenoids from Trichodesma amplexicaule Roth.

Singh

2003

Phytotherapy Research

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“…This preparative TLC was repeated until sufficient crystallizable quantities could be obtained of each of the substance (Fried and Sherma, 1986). Each of the isolated compounds was crystallized with methanol: acetone (1:2; Kaul et al, 1967). The standards were obtained from the Natural Products Laboratory, Department of Chemistry, University of Rajasthan, Jaipur.…”

Section: Quantification Of Triterpenoidsmentioning

confidence: 99%

“…The spots separated by TLC were scraped from the plate with sorbent and the compound was eluted with chloroform and concentrated (Coscia, 1984). Each of the compounds was crystallized with methanol : acetone (1:2; Kaul et al, 1967). These crystals were dried in vacuo and subjected to various physical and spectral studies for characterization (Heilbron and Bunbury, 1953;Yamaguchi, 1970).…”

Section: Quantification Of Triterpenoidsmentioning

confidence: 99%

Estimation of triterpenoids from Heliotropium marifolium Koen. ex Retz. in vivo and in vitro. I. Antimicrobial screening

Singh

2001

Phytotherapy Research

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The hexane extract of Heliotropium marifolium yielded a mixture of triterpenoids: beta-sitosterol, stigmasterol, beta-amyrin, friedelan-3beta-ol (epifriedelenol), cycloartenone, beta-amyrin acetate, friedelin and epifriedenyl acetate. Isolated triterpenoid and reference antibiotics (gentamycin/mycostatin) were tested against selected pathogenic bacteria and fungi, e.g. Escherichia coli, Staphylococcus aureus, Aspergillus niger and Penicillium chrysogenum. The inhibition zone (IZ) and the activity index (AI) of isolated compounds were recorded and it was found that epifriedenyl acetate (IZ = 17; AI = 1.06) was the most active. The present study deals with the quantification and assessment of their growth inhibitory potency. It has been reported that cycloartenone was the major triterpenoid in both in vivo (0.54%) and in vitro (0.11%) cell cultures.

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“…Cardioactive glycosides have been isolated from the bulb of white squill plant, Urginea maritima (LINNB) BAKER [I], and they have been structurally elucidated [I-31 and pharmacologically examined [4-71. KAUL et al reported that semipurified extracts of a squill tissue culture established from withe squill bulbs produced a pronounced vasodilatation and bradycardia in anaesthetized rabbits [8]. T h e objective of this study was to examine similar cemi-purified extracts for the presence of bufadienolides, anthocyanins and other compounds.…”

Section: Introductionmentioning

confidence: 95%

Examination of Squill Tissue Cultures for Bufadienolides and Anthocyanins

Se¹,

Ej²

1976

Planta Med

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Three major compounds were isolated but not crystallized by thin-layer chromatography from the previously reported cardioactive semi-purified extracts o f Urginea maritima (LINNB) BAKER (white squill) tissue cultures. Compounds separated were different from the standard squill bufadienolides by ultraviolet, infrared and mass spectra. N o bufadienolides were identified in the tissue cultures studied.Red-purple pigments were produced in squill tissue cultures grown under light. These pigments were tentatively identified by absorption spectra and color reactions as anthocyanins containing a catechol group. N o such pigments were found in squill tissue cultures grown in the dark.

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Production of cardio-active substances by plant tissue cultures and their screening for cardiovascular activity

Cited by 19 publications

References 5 publications

Antimicrobial activity of terpenoids from Trichodesma amplexicaule Roth.

Antimicrobial activity of terpenoids from Trichodesma amplexicaule Roth.

Estimation of triterpenoids from Heliotropium marifolium Koen. ex Retz. in vivo and in vitro. I. Antimicrobial screening

Examination of Squill Tissue Cultures for Bufadienolides and Anthocyanins

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