Production of cellulases and xylanases in solid-state fermentation by different strains of Aspergillus niger using sugarcane bagasse and brewery spent grain

Morán-Aguilar, María Guadalupe; Costa-Trigo, Iván; Calderón‐Santoyo, Montserrat; Domínguez, José Manuel; Aguilar‐Uscanga, María Guadalupe

doi:10.1016/j.bej.2021.108060

Cited by 77 publications

(30 citation statements)

References 51 publications

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“…The application of such strategies that use cheap renewable sources of C and N are a direct contribution to the reduction of the costs of raw materials, which accounts for 30–40% of the total enzyme production cost. This can, consequently, positively impact on cost reductions of the saccharifying enzymes, overcoming a major barrier for industrial scale‐up and commercial use of lignocellulose materials in a biorefinery context 24,25 …”

Section: Discussionmentioning

confidence: 99%

“…This can, consequently, positively impact on cost reductions of the saccharifying enzymes, overcoming a major barrier for industrial scale-up and commercial use of lignocellulose materials in a biorefinery context. 24,25 The influence of substrate concentration also was assessed by processing pretreated BSG at 7% (10 g L −1 xylan equivalent) against the 11% (15 g L −1 xylan equivalent) mentioned above, both with CSL/KNO 3 supplementation. The reduction in the substrate concentration led to a small decrease of xylanase specific activities from 57.3 to 43.8 U mg −1 xylan, respectively.…”

Section: Discussionmentioning

confidence: 99%

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High cellulase‐free xylanases production by Moesziomyces aphidis using low‐cost carbon and nitrogen sources

Faria

Marques

Cerejo

et al. 2022

J of Chemical Tech & Biotech

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Background: Enzymes involved in xylan hydrolysis have several industrial applications. Selection of efficient microbial hosts and scalable bioreaction operations can lower enzyme production costs and contribute to their commercial deployment. This work aims at investigating the Moesziomyces aphidis yeast cultivation conditions that deliver maximal xylanase titres, yields and productivities using low-cost nitrogen (N) and carbon (C) sources.Results: NaNO 3 and KNO 3 supplementation improved xylanase production 2.9-and 2.7-fold (against 67.2 U mL −1 ), respectively, using xylan as C source. Interestingly, the use of KNO 3 , instead of NaNO 3 , results in 2-to 3-fold higher specific activity, highlighting the potassium ion role. In addition, this study investigates synergetic effects on using ionic and organic N sources. A 4.9-fold increase in xylanase production, with high specific activity, is attained combining KNO 3 and corn steep liquor (CSL). Exploring the previous findings, this study reports one of the highest extracellular xylanase production titres (864.7 U mL −1 ) by yeasts, using a media formulation containing dilute-acid pre-treated brewery spent grains (BSG), as C source and inducer, supplemented with KNO 3 and CSL. Replacement of dilute-acid pre-treatmed BSG by untreated BSG had low impact on xylanase production, of only 6%.Conclusion: Efficient production of M. aphidis xylanolytic enzymes, using low-cost N and C sources, is attractive for deployment of on-site enzyme production targeting different biotechnological applications under circular economy and biorefinery concepts. Potential xylanases end-users include industries such as brewing (using BSG as substrate for enzyme production), pulp and paper (benefiting from the cellulase-free xylanase activity) or lignocellulosic ethanol (for cellulase supplementation).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

High cellulase‐free xylanases production by Moesziomyces aphidis using low‐cost carbon and nitrogen sources

Faria

Marques

Cerejo

et al. 2022

J of Chemical Tech & Biotech

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“…Fermentation occurred at 30 • C for 7 days without mixing. The highest cellulase activity was 6.23 U/g of dry substrate (gds) using BSG pretreated with boiling water, whereas the highest xylanase activity was 1400.80 U/gds obtained using BSG pretreated in an autoclave [66]. Another study reported the ability of A. fumigatus and Penicillium sp.…”

Section: Valorization Of Bsg Using Solid State-fermentationmentioning

confidence: 99%

Valorization of Brewers’ Spent Grains: Pretreatments and Fermentation, a Review

et al. 2022

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Brewers’ spent grains constitute a valuable byproduct of the beer industry. They are characterized by a rich nutritional composition consisting of around 70% lignocellulosic fibrous material, 20% proteins, 10% lipids, in addition to vitamins, minerals, amino acids, and phenolic compounds. These spent grains are produced in large amounts all through the year, are cheap, and lack economically feasible applications. Nowadays, 70% of these spent grains are used as animal feed, 10% are used for biogas production, and the remaining 20% are disposed in landfills. Due to the aforementioned facts, alternative uses of the brewers’ spent grains are highly sought-after. In fact, this nutrient-rich industrial by-product makes it a very good candidate for valorization through biotechnological processing, particularly microbial fermentation. After applying the needed pretreatments, using brewers’ spent grains as a substrate in submerged and solid-state fermentation of different microorganisms leads to the production of various value-added compounds such as organic acids, amino acids, volatile fatty acids, enzymes, vitamins, second-generation biofuels and other products.

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“…The global rate of the process is influenced by the structural characteristics of the biomass and the origin of the enzymes [ 12 ]. However, this stage can be a limitation for the biorefinery process given the high cost and thermostability of enzymes available on the market, as well as the low conversion yields due to the characteristics of lignocellulosic materials [ 10 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids

Outeiriño

Costa-Trigo

Oliveira

et al. 2022

Foods

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Novel environmentally friendly pretreatments have been developed in recent years to improve biomass fractionation. Solid-state fermentation (SSF) and treatment with ionic liquids show low environmental impact and can be used in biorefinery of biomass. In this work, these processes were assessed with brewery spent grain (BSG). First, BSG was used as a substrate to produce cellulases and xylanases by SSF with the fungi Aspergillus brasiliensis CECT 2700 and Trichoderma reesei CECT 2414. Then, BSG was pretreated with the ionic liquid [N1112OH][Gly] and hydrolyzed with the crude enzymatic extracts. Results showed that SSF of BSG with A. brasiliensis achieved the highest enzyme production; meanwhile, the pretreatment with ionic liquids allowed glucan and xylan fractions to increase and reduce the lignin content. In addition, a mixture of the extracts from both fungi in a ratio of 2.5:0.5 Aspergillus/Trichoderma (v/v) efficiently hydrolyzed the BSG previously treated with the ionic liquid [N1112OH][Gly], reaching saccharification percentages of 80.68%, 54.29%, and 19.58% for glucan, xylan, and arabinan, respectively. In conclusion, the results demonstrated that the BSG biorefinery process developed in this work is an effective way to obtain fermentable sugar-containing solutions, which can be used to produce value-added products.

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Production of cellulases and xylanases in solid-state fermentation by different strains of Aspergillus niger using sugarcane bagasse and brewery spent grain

Cited by 77 publications

References 51 publications

High cellulase‐free xylanases production by Moesziomyces aphidis using low‐cost carbon and nitrogen sources

High cellulase‐free xylanases production by Moesziomyces aphidis using low‐cost carbon and nitrogen sources

Valorization of Brewers’ Spent Grains: Pretreatments and Fermentation, a Review

Biorefinery of Brewery Spent Grain by Solid-State Fermentation and Ionic Liquids

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