Production of Cellulases by Bacillus cellulosilyticus
Using Lignocellulosic Material

Maryam, Bushra; Qadir, Abdul; Zameer, Mariam; Ahmad, Sajid Rashid; Nelofer, Rubina; Jamil, Nadia; Arzoo, Sameen; Afzaal, Rubab

doi:10.15244/pjoes/80867

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…However, the enzyme activity shown by this isolate was found to be poor when compared to the other isolates under the study. The data are comparable to the findings reported by Maryam et al [32] on Bacillus cellulosilyticus which showed optimum cellulase production at pH 5 using alkali pretreated substrate as a source of carbon. Nandimath et al [33] isolated Bacillus and Pseudomonas species having cellulolytic potential.…”

Section: Optimization Of Cellulase Productionsupporting

confidence: 89%

Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production

Bhagat¹,

Kokitkar²

2021

J App Biol Biotech

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Soil is one of the most promising sources for the presence of a variety of microorganisms which produce different hydrolytic enzymes. Such microorganisms include bacteria, fungi, etc. The ability to produce hydrolytic enzymes makes them potential candidates for hydrolysis of complex polymeric substrates. The present study aims at screening, isolating, and characterizing cellulolytic bacteria isolated from soil samples. Nine different soil samples were collected from different locations near Raigad district, Maharashtra, India. Carboxymethylcellulose (CMC) was used as a sole source of carbon for screening of cellulase-producing isolates. Forty-five different cellulase-producing bacteria were isolated based on their ability to decolorize Congo red and iodine. The morphological and molecular characterization of seven best isolates was carried out for their identification. All seven isolates were identified to be Bacillus species using 16s rRNA gene-based sequencing. The optimization of cellulase enzyme production of these seven isolates was carried out by using different parameters such as pH, temperature, and carbon sources. Majority of the cellulase producers identified in the present research work were found to be mesophiles. pH ranging from 6 to 8 was found to be most suitable to produce cellulase enzyme by the isolates. The data suggest that polymeric substances such as starch and CMC act as inducers for cellulase production.

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Section: Optimization Of Cellulase Productionsupporting

confidence: 89%

Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production

Bhagat¹,

Kokitkar²

2021

J App Biol Biotech

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“…Our results indicated that isolates from the Terfez rhizosphere and those from thermal waters performed best with a CMCase activity of 0.7 to 2.2 U/mL. These results should be compared with those of Maryam et al [27], who indicated that the CMCasic activity is 1.99 U/mL for B. cellulosillyticus and lower by 0.07 U/mL for B. subtilis AS3 [28]. However, other strains of Bacillus are capable of having a higher endoglucanase activity, from 7.3 U/mL to 9.1 U/mL [29].…”

Section: Discussionsupporting

confidence: 53%

“…The concentration of total sugars varied relatively in the same way for all the strains, and it varied from 335 µg/mL to 450 µg/mL; these monomeric sugars are produced from the hydrolysis of the substrate (CMC), which reflects that this substrate is highly digestible by the strains tested. The estimate of the total sugar content in a culture supernatant on medium containing lignocellulosic materials from a strain of B. cellulosilyticus is 179.84 mg/mL [27].…”

Section: Discussionmentioning

confidence: 99%

Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

et al. 2022

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Cellulose represents the most abundant component of plant biomass on earth; it is degraded by cellulases, specific enzymes produced by microorganisms. However, cellulases of bacterial origin attract more interest due to their natural diversity and ability to inhabit a variety of niches, allowing the selection of cellulolytic strains resistant to environmental stresses. The screening of the cellulolytic activity of 398 bacteria isolated from various ecosystems in Algeria (cave, ruins, chott, thermal station, and rhizosphere of arid and semi-arid regions) was performed by the appearance of a hydrolysis zone on carboxymethylcellulose (CMC) medium. The cellulase activity on CMC (1%) broth allowed to select 26 strains among which 12 had the best activity (0.3 U/mL to 2.2 U/mL). Optimization of physicochemical parameters (salinity: 0–1 M NaCl; pH: 3, 4, 7, 9, and 11; temperature: 30, 45, and 50 °C; PEG8000: 0 and 30%) involved in growth and cellulose production showed that the majority of strains were mesophilic, neutrophilic, or alkali- tolerant and tolerant to 30% of PEG8000. The cellulase activity and stability under different stress allowed to retain five strains, which the most efficient. Based on the 16S-rRNA sequencing results, they belonged to the genus Bacillus. The physicochemical properties of cellulases (crude extract) showed a CMCase active over a wide range of pH (4 to 11), optimal at 50 °C and 60 °C. The inhibiting salinity effect on the activity was not detected and was negligible on the enzymatic stability. The residual CMCase activity remained between 40 and 70% in a temperature range between 40 and 70 °C, was stable over a wide range of saline concentrations (0–2000 mM), and was weakly affected at 30% of PEG8000. The crude enzyme extract was able to hydrolyze both soluble and insoluble cellulosic substrates. The evaluation of the hydrolysis capacity of lignocellulosic waste revealed the ability of tested strains to degrade wheat bran, barley bran, and corncob. In addition, the enzyme showed significant multi-stress resistance on solid and liquid media. By these characteristics, these cellulolytic bacteria could be attractive to be used in various industrial and biotechnology applications.

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“…This study is of its kind to report the cellulase producing bacteria from saw dust which is a by product of wood working areas. Furthermore, using 16S rRNA confirmed that one of the isolates is B. stratosphericus, which is for the first time Studies reported that temperature greatly affect growth of microbes and ultimately extra cellular enzyme production [21,22]. Keeping in view, different conditions based on variation in temperature range was provided to the selected strains.…”

Section: Discussionmentioning

confidence: 64%

Evaluation, Characterization and Molecular Analysis of Cellulolytic Bacteria from Soil in Peshawar, Pakistan

Ikram

Khan

Ali

et al. 2022

Microbiol. Biotechnol. Lett.

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Cellulases are a group of biocatalyst enzymes that are capable of degrading cellulosic biomass present in the natural environment and produced by a large number of microorganisms, including bacteria and fungi, etc. In the current study, we isolated, screened and characterized cellulase-producing bacteria from soil. Three cellulose-degrading species were isolated based on clear zone using Congo red stain on carboxymethyl cellulose (CMC) agar plates. These bacterial isolates, named as HB2, HS5 and HS9, were subsequently characterized by morphological and biochemical tests as well as 16S rRNA gene sequencing. Based on 16S rRNA analysis, the bacterial isolates were identified as Bacillus cerus, Bacillus subtilis and Bacillus stratosphericus. Moreover, for maximum cellulase production, different growth parameters were optimized. Maximum optical density for growth was also noted at pH 7.0 for 48 h for all three isolates. Optical density was high for all three isolates using meat extract as a nitrogen source for 48 h. The pH profile of all three strains was quite similar but the maximum enzyme activity was observed at pH 7.0. Maximum cellulase production by all three bacterial isolates was noted when using lactose as a carbon rather than nitrogen and peptone. Further studies are needed for identification of new isolates in this region having maximum cellulolytic activity. Our findings indicate that this enzyme has various potential industrial applications.

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Production of Cellulases by Bacillus cellulosilyticus Using Lignocellulosic Material

Cited by 5 publications

References 40 publications

Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production

Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production

Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

Evaluation, Characterization and Molecular Analysis of Cellulolytic Bacteria from Soil in Peshawar, Pakistan

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