Production of cellulolytic enzymes from theXylaria andHypoxylon species of xylariaceae

Wei, Ding; Chang, Shan; Wei, Yau‐Huei; Lin, Yao‐Xin; Chuang, Chih-Yu; Jong, S. C.

doi:10.1007/bf01195834

Cited by 22 publications

(7 citation statements)

References 12 publications

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“…from Hevea brasiliensis . Wei et al [33] cultivated an A. stigyum strain on Avicel and confirmed the production of β-glucosidase, although only low levels of cellulases were detected.…”

Section: Discussionmentioning

confidence: 99%

The capability of endophytic fungi for production of hemicellulases and related enzymes

et al. 2013

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BackgroundThere is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw material uses for the production of ethanol second generation (2G). New cellulolytic and hemicellulytic enzymes are needed, in order to optimize the degradation of bagasse and production of ethanol 2G.ResultsThe ability to produce hemicellulases and related enzymes, suitable for lignocellulosic biomass deconstruction, was explored using 110 endophytic fungi and 9 fungi isolated from spoiled books in Brazil. Two initial selections were performed, one employing the esculin gel diffusion assay, and the other by culturing on agar plate media with beechwood xylan and liquor from the hydrothermal pretreatment of sugar cane bagasse. A total of 56 isolates were then grown at 29°C on steam-exploded delignified sugar cane bagasse (DEB) plus soybean bran (SB) (3:1), with measurement of the xylanase, pectinase, β-glucosidase, CMCase, and FPase activities. Twelve strains were selected, and their enzyme extracts were assessed using different substrates. Finally, the best six strains were grown under xylan and pectin, and several glycohydrolases activities were also assessed. These strains were identified morphologically and by sequencing the internal transcribed spacer (ITS) regions and the partial β-tubulin gene (BT2). The best six strains were identified as Aspergillus niger DR02, Trichoderma atroviride DR17 and DR19, Alternaria sp. DR45, Annulohypoxylon stigyum DR47 and Talaromyces wortmannii DR49. These strains produced glycohydrolases with different profiles, and production was highly influenced by the carbon sources in the media.ConclusionsThe selected endophytic fungi Aspergillus niger DR02, Trichoderma atroviride DR17 and DR19, Alternaria sp. DR45, Annulohypoxylon stigyum DR47 and Talaromyces wortmannii DR49 are excellent producers of hydrolytic enzymes to be used as part of blends to decompose sugarcane biomass at industrial level.

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“…from Hevea brasiliensis . Wei et al [33] cultivated an A. stigyum strain on Avicel and confirmed the production of β-glucosidase, although only low levels of cellulases were detected.…”

Section: Discussionmentioning

confidence: 99%

The capability of endophytic fungi for production of hemicellulases and related enzymes

et al. 2013

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“…Xylaria has been proved to be a good source of bioactive compounds. Wei et al (1992) screened twelve Xylaria species and five Hypoxylon species which produce cellulolytic enzymes. Liu et al (2008) isolated an endophytic Xylaria.…”

Section: Identification Of 48 Strains Belonging To Sordariomycetesmentioning

confidence: 99%

Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis

Chen

Wei

et al. 2010

World J Microbiol Biotechnol

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Fifty-two endophytic fungi strains with different colony morphologies were isolated from stems, leaves and roots of Huperzia serrata (Thunb. ex Murray) Trevis. collected from Bawangling Reserve of Hainan Province in southern China. They were identified mainly based on rDNA ITS sequences and phylogenetic analysis. The results showed that all strains belonged to four classes, i.e. Sordariomycetes (92.31%), Dothideomycetes (3.85%), Pezizomycetes (1.92%) and Agaricomycetes (1.92%). Forty-seven strains were identified at the genus level, including Glomerella (Colletotrichum), Hypocrea (Trichoderma), Pleurostoma, Chaetomium, Coniochaeta (Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus. As to the other five strains, three were identified at the order level and two at the family level, indicating that a great diversity of fungi taxa exists in H. serrata. Most isolated strains belonged to the genus of Glomerella (Colletotrichum) and Hypoxylon, twenty-one from Glomerella and its anamorph Colletotrichum (42.3% of total isolated strains) and ten from Hypoxylon (19.2% of total isolated strains). Pleurostoma, Chaetomium, Coniochaeta (Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus were reported as endophytic fungi isolated from H. serrata for the first time.

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“…Many of the Xylariaceae are saprotrophs on decaying plant material such as logs and stumps, dead twigs and branches of trees, dead leaves and stems of herbaceous plants (Whalley, 1996). It is also clear that many species can degrade lignin (Sutherland & Crawford, 1981) and that others exhibit impressive production of cellulolytic enzymes (Wei et al , 1992). Daldinia ( Xylariaceae ) is a genus of wood‐inhabiting pyrenomycetes with perithecia embedded in large stromata that are internally concentrically zoned and produce enzymes that digest the cell wall components for nutrition and energy (Johannesson et al , 2000).…”

Section: Introductionmentioning

confidence: 99%

Purification and biochemical characterization of an extracellular Î²-glucosidase from the wood-decaying fungusDaldinia eschscholzii(Ehrenb.:Fr.) Rehm

Karnchanatat

Petsom

Sangvanich

et al. 2007

FEMS Microbiology Letters

113

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An extracellular beta-glucosidase was purified from culture filtrates of the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm grown on 1.0% (w/v) carboxymethyl-cellulose using ammonium sulfate precipitation, ion-exchange, hydrophobic interaction and gel filtration chromatography. The enzyme is monomeric with a molecular weight of 64.2 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and has a pI of 8.55. The enzyme catalyzes the hydrolysis of p-nitrophenyl-beta-D-glucopyranoside (PNPG) as the substrate, with a K(m) of 1.52 mM, and V(max) of 3.21 U min mg(-1) protein. Glucose competitively inhibited beta-glucosidase with a K(i) value of 0.79 mM. Optimal activity with PNPG as the substrate was at pH 5.0 and 50 degrees C. The enzyme was stable at pH 5.0 at temperatures up to 50 degrees C. The purified beta-glucosidase was active against PNPG, cellobiose, sophorose, laminaribiose and gentiobiose, but did not hydrolyze lactose, sucrose, Avicel or o-nitrophenyl-beta-d-galactopyranoside. The activity of beta-glucosidase was stimulated by Ca(2+), Co(2+), Mg(2+), Mn(2+), glycerol, dimethyl sulfoxide (DMSO), dithiothreitol and EDTA, and strongly inhibited by Hg(2+). The internal amino acid sequences of D. eschscholziibeta-glucosidase have similarity to the sequences of the family 3 beta-glucosyl hydrolase.

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Production of cellulolytic enzymes from theXylaria andHypoxylon species of xylariaceae

Cited by 22 publications

References 12 publications

The capability of endophytic fungi for production of hemicellulases and related enzymes

The capability of endophytic fungi for production of hemicellulases and related enzymes

Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis

Purification and biochemical characterization of an extracellular Î²-glucosidase from the wood-decaying fungusDaldinia eschscholzii(Ehrenb.:Fr.) Rehm

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