Production of chitin from red crab shell waste by successive fermentation with Lactobacillus paracasei KCTC-3074 and Serratia marcescens FS-3

Jung, Woo‐Jin; Jo, Gyung-Hyun; Kuk, Ju-Hee; Kim, Y.J.; Oh, Kyung-Taek; Park, R.D.

doi:10.1016/j.carbpol.2006.08.011

Cited by 95 publications

(53 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chemical treatment requires the use of HCl and NaOH, which can cause deacetylation and depolymerization of chitin. Many reports have demonstrated the application of proteolytic microorganisms for the deproteinization of marine crustacean wastes to produce chitin [9,10,30,38]. However, few studies on the use of proteolytic enzymes for the deproteinization of crustacean wastes have been reported.…”

Section: Effect Of Organic Solvents On Enzyme Stabilitymentioning

confidence: 97%

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

Manni

Jellouli

Ghorbel-Bellaaj

et al. 2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. In this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by Bacillus cereus SV1. Maximum protease activity (5,900 U/mL) was obtained when the strain was grown in medium containing 40 g/L shrimp wastes powder as a sole carbon source. The optimum pH, optimum temperature, pH stability, and thermal stability of the crude enzyme preparation were pH 8.0, 60 degrees C, pH 6-9.5, and <55 degrees C, respectively. The crude protease was extremely stable toward several organic solvents. No loss of activity was observed even after 60 days of incubation at 30 degrees C in the presence of 50% (v/v) dimethyl sulfoxide and ethyl ether; the enzyme retained more than 70% of its original activity in the presence of ethanol and N,N-dimethylformamide. The protease showed high stability toward anionic (SDS) and non-ionic (Tween 80, Tween 20, and Triton X-100) surfactants. Interestingly, the activity of the enzyme was significantly enhanced by oxidizing agents. In addition, the enzyme showed excellent compatibility with some commercial liquid detergents. The protease of B. cereus SV1, produced under the optimal culture conditions, was tested for shrimp waste deproteinization in the preparation of chitin. The protein removal with a ratio E/S of 20 was about 88%. The novelties of the SV1 protease include its high stability to organic solvents and surfactants. These unique properties make it an ideal choice for application in detergent formulations and enzymatic peptide synthesis. In addition, the enzyme may find potential applications in the deproteinization of shrimp wastes to produce chitin.

show abstract

Section: Effect Of Organic Solvents On Enzyme Stabilitymentioning

confidence: 97%

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

Manni

Jellouli

Ghorbel-Bellaaj

et al. 2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

show abstract

“…Chemical treatment requires the use of HCl and NaOH, which can cause chitin deacetylation and depolymerization. Many reports have demonstrated the application of proteolytic microorganisms for the deproteinization of marine crustacean wastes to produce chitin [17,18,50,51]. However, few studies on the use of proteolytic enzymes for the deproteinization of crustacean wastes have been reported [20].…”

Section: Effect Of Organic Solvents On Enzyme Stabilitymentioning

confidence: 97%

Solvent-Stable Digestive Alkaline Proteinases from Striped Seabream (Lithognathus mormyrus) Viscera: Characteristics, Application in the Deproteinization of Shrimp Waste, and Evaluation in Laundry Commercial Detergents

Ali

Hmidet

Ghorbel-Bellaaj

et al. 2011

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Alkaline proteases from the viscera of the striped seabream (Lithognathus mormyrus) were extracted and characterized. Interestingly, the crude enzyme was active over a wide range of pH from 6.0 to 11.0, with an optimum pH at the range of 8.0-10.0. In addition, the crude protease was stable over a broad pH range (5.0-12.0). The optimum temperature for enzyme activity was 50 °C. The crude alkaline proteases showed stability towards various surfactants and bleach agents and compatibility with some commercial detergents. It was stable towards several organic solvents and retained more than 50% of its original activity after 30 days of incubation at 30 °C in the presence of 25% (v/v) dimethyl sulfoxide, N,N-dimethylformamide, diethyl ether, and hexane. The crude enzyme extract was also tested for shrimp waste deproteinization in the preparation of chitin. The protein removal with a ratio enzyme/substrate of 10 was about 79%.

show abstract

“…More particularly, the protonated chitin increased its bonding with the negatively charged SO 3 À of R40 and Y4, and thus strengthened its adsorptive capacity on R40 and Y4. Chitin and its deacetylated form, chitosan, are widely used in the food and cosmetic industries, medical products, agriculture, wastewater treatment, and membranes because of its biodegradability, biocompatibility and nontoxicity (Jung et al, 2007;Wang, 2012). Preparation of chitin or chitosan from marine chitin-containing by-products (shrimp shell, crab shell, and squid pen) by chemical processes involves deproteinization and demineralization with the use of strong bases and acids.…”

Section: Adsorption Effects On Food Colourants Of R40 and Y4mentioning

confidence: 99%

“…paracasei and S. marcescens have been used for the production of chitin from crab shell waste by successive fermentation (Jung et al, 2007). In our previous study, S. marcescens TKU011 as a protease-, chitosanase-and chitinase-producing bacterium was employed to degrade squid pen to produce N-acetyl chitooligosaccharides in liquid phase fermentation (Wang, Yang, Liang, Peng, & Wang, 2009).…”

Section: Introductionmentioning

confidence: 99%

Utilisation of chitinous materials in pigment adsorption

et al. 2012

View full text Add to dashboard Cite

Production of chitin from red crab shell waste by successive fermentation with Lactobacillus paracasei KCTC-3074 and Serratia marcescens FS-3

Cited by 95 publications

References 9 publications

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

Solvent-Stable Digestive Alkaline Proteinases from Striped Seabream (Lithognathus mormyrus) Viscera: Characteristics, Application in the Deproteinization of Shrimp Waste, and Evaluation in Laundry Commercial Detergents

Utilisation of chitinous materials in pigment adsorption

Contact Info

Product

Resources

About