Production of chitinase from Escherichia fergusonii, chitosanase from Chryseobacterium indologenes, Comamonas koreensis and its application in N-acetylglucosamine production

Kim, Tae Il; Lim, Dong Hyun; Baek, Ki Hyun; Jang, Sun Sik; Park, Beom Young; Vijayakumar, M.

doi:10.1016/j.ijbiomac.2018.02.056

Cited by 21 publications

(8 citation statements)

References 29 publications

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“…Using the deacetylase enzyme which isolated from Cyclobacteriummarinum and these can all be used to convert the GlcNAc to glucosamine following two-steps strategies: (a) glycosidic bound cleavage of chitin to give intermediate GlcNAc by enzymatic processes, and (b) through selective enzyme which has ability for N-deacetylation GlcNAc to GlcN with high yield (Scheme 4) [54]. The GlcNAc is particularly obtained with acidic hydrolysis of chitin as in (Scheme 4), the GlcNAc converted to GlcN as intermediate [51,[55][56][57][58]. However, Petiard et al [59] reported that the extraction of GlcN from plants investigated with high temperature and low yield (Scheme 4).…”

Section: Biocatalytic Potential Of Enzymes Involved the Modification Of Glucosamine In Chemotherapeuticalmentioning

confidence: 99%

Biocatalytic Potential of Enzymes Involved the Modification of Glucosamine in Chemotherapeutical Intermediates Synthesis

Senan¹

2021

J Food Chem Nanotechnol

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The enzymatic modification of glucosamine (GlcN) and its derivatives have a keen interest for researchers due to the natural abundance of its bio-sourced such as chitin. The recent progress for understanding the role of glucosamine based on various novel approaches for enzymatic synthesis in the field of medicinal chemistry and food supplements. These developments led to improve chemo-selective protection and/or deprotection of amino-sugar during chainenhancement and allow the simultaneous regioselective reaction of functionally relevant acetyl, phosphate, or sulfate groups. In this review, we give an overview of current strategies on the synthesis of therapeutically relevant glucosaminecontaining derivatives.

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Section: Biocatalytic Potential Of Enzymes Involved the Modification Of Glucosamine In Chemotherapeuticalmentioning

confidence: 99%

Biocatalytic Potential of Enzymes Involved the Modification of Glucosamine in Chemotherapeutical Intermediates Synthesis

Senan¹

2021

J Food Chem Nanotechnol

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“…Various techniques have thus far been used for the production of aminooligosaccharides from chitinous biomass, including chemical (Kazami et al, 2015), physical (Ajavakom et al, 2012), and enzymatic methods (Tae et al, 2018). Compared with the highpolluting chemical degradation and the high-energy-cost physical degradation, the enzymatic method is superior due to its mild reaction condition, high yield, low side reaction and minor pollution (Joseph et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

A Novel GH Family 20 β-N-acetylhexosaminidase With Both Chitosanase and Chitinase Activity From Aspergillus oryzae

Zhang

Qin

et al. 2021

Front. Mol. Biosci.

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Aminooligosaccharides possess various biological activities and can exploit wide applications in food, pharmaceutical and cosmetic industries. Commercial aminooligosaccharides are often prepared by the hydrolysis of chitin and chitosan. In this study, a novel GH family 20 β-N-acetylhexosaminidases gene named AoNagase was cloned from Aspergillus oryzae and expressed in Pichia pastoris. The purified AoNagase had maximal activity at pH 5.5 and 65°C. It exhibited good pH stability in the range of pH 6.0–7.5 and at temperatures below 50°C. AoNagase was capable of hydrolyzing not only colloidal chitosan (508.26 U/mg) but also chitin (29.78 U/mg). The kinetic parameters (Km and Vmax) of AoNagase were 1.51 mM, 1106.02 U/mg for chitosan and 0.41 mM, 40.31 U/mg for colloidal chitin. To our knowledge, AoNagase is the first GH family 20 β-N-acetylhexosaminidase capable of hydrolyzing both chitosan and chitin. AoNagase is an endo-type β-N-acetylhexosaminidases and can potentially be used for the manufacturing of aminooligosaccharides.

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“…It also has wide applications in food, cosmetics, and pharmaceutical industries (Liu et al, 2013;Zhou et al, 2019). Traditionally, N-acetylglucosamine is produced from chitin by chemical hydrolysis using strong acids, which can cause severe environmental problems and has potential risk of allergic reactions (Liu et al, 2019;Kim et al, 2018;Arbia et al, 2013). Enzymatic method to produce N-acetylglucosamine has also been done.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Providencia stuartii Cells in Papaya Trunk Wood for N-acetylglucosamine Production from Pennaeus vannamei Shrimp Shells

Halim

Tantradjaja

Hardoko

et al. 2021

JIPK

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Highlight Research AbstractChitin is a natural compound found abundantly in shrimp shells. Chitin can be degraded to produce N-acetylglucosamine, which has wide applications in the food and pharmaceutical fields. Fermentation using chitinolytic microorganisms can be used to produce N-acetylglucosamine from shrimp shells’ chitin. One of the strong chitinolytic bacteria that was isolated from previous research was Providencia stuartii. To provide better stability and efficiency in fermentation, P. stuartii cells were immobilized using entrapment method in papaya trunk wood. The aims of this research were to determine the optimum papaya trunk wood size, ratio of papaya trunk wood and growth medium, as well as the optimum fermentation cycle to produce N-acetylglucosamine from P. vannamei shrimp shells using submerged fermentation method. The research used experimental method with treatment of different sizes of papaya trunk wood (1 x 1 x 1 cm3, 1.5 x 1.5 x 1.5 cm3, and 2 x 2 x 2 cm3), different ratio of papaya trunk wood and growth medium (1:10, 1:15 and 1:20), and 4 fermentation cycles. Results showed that papaya trunk wood with size of 1 x 1 x 1 cm3 and ratio (w/v) of 1:10 could immobilize 87.08±2.05% of P. stuartii cells and produce the highest N-acetylglucosamine concentration, which was 238177.78±3153.48 ppm. The highest N-acetylglucosamine production was obtained from first fermentation cycle and decreased over the last three cycles, but still produced high concentration of N-acetylglucosamine. Therefore, it is possible to perform continuous N-acetylglucosamine production from shrimp shells using P. stuartii cells immobilized in papaya trunk wood.

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Production of chitinase from Escherichia fergusonii, chitosanase from Chryseobacterium indologenes, Comamonas koreensis and its application in N-acetylglucosamine production

Cited by 21 publications

References 29 publications

Biocatalytic Potential of Enzymes Involved the Modification of Glucosamine in Chemotherapeutical Intermediates Synthesis

Biocatalytic Potential of Enzymes Involved the Modification of Glucosamine in Chemotherapeutical Intermediates Synthesis

A Novel GH Family 20 β-N-acetylhexosaminidase With Both Chitosanase and Chitinase Activity From Aspergillus oryzae

Immobilization of Providencia stuartii Cells in Papaya Trunk Wood for N-acetylglucosamine Production from Pennaeus vannamei Shrimp Shells

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