Production of Clinical-Type Dextran - Partial Hydrolytic Depolymerization and Fractionation of the Dextran from Leuconostoc mesenteroides Strain NRRL B-512

Wolff, I. A.; Mehltretter, C. L.; Mellies, R. L; Watson, P. R.; Hofreiter, B. T.; Piras, Patrick; Rist, C. E.

doi:10.1021/ie50530a047

Cited by 31 publications

(14 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During fermentation, the pH drops from 7 to 5 due to the generation of lactic acid; therefore, non-ionic detergents are usually added to maintain the stability of the bacteria and its enzymes [8]. In the clinical area, dextran is usually obtained from the acid hydrolysis (e.g., sulfuric and hydrochloric acids) of the native dextran from Leuconostoc mesenteroides NRRL B512, which allows controlling the molecular weights of the resulting dextrans [26,27].…”

Section: Synthesis Of Dextranmentioning

confidence: 99%

Dextran: Sources, Structures, and Properties

Díaz‐Montes

2021

Polysaccharides

149

View full text Add to dashboard Cite

Dextran is an exopolysaccharide (EPS) synthesized by lactic acid bacteria (LAB) or their enzymes in the presence of sucrose. Dextran is composed of a linear chain of d-glucoses linked by α-(1→6) bonds, with possible branches of d-glucoses linked by α-(1→4), α-(1→3), or α-(1→2) bonds, which can be low (<40 kDa) or high molecular weight (>40 kDa). The characteristics of dextran in terms of molecular weight and branches depend on the producing strain, so there is a great variety in its properties. Dextran has commercial interest because its solubility, viscosity, and thermal and rheological properties allow it to be used in food, pharmaceutical, and research areas. The aim of this review article is to compile the latest research (in the past decade) using LAB to synthesize high or low molecular weight dextran. In addition, studies using modified enzymes to produce dextran with specific structural characteristics (molecular weights and branches) are addressed. On the other hand, special attention is paid to LAB extracted from unconventional sources to expose their capacities as dextran producers and their possible application to compete with the only commercial strain (Leuconostoc mesenteroides NRRL B512).

show abstract

Section: Synthesis Of Dextranmentioning

confidence: 99%

Dextran: Sources, Structures, and Properties

Díaz‐Montes

2021

Polysaccharides

149

View full text Add to dashboard Cite

show abstract

“…The relative similarity of the results of number and weight average methods indicates that while the individual fractions were not homogeneous they were considerably more so than the native dextrans, but similar to the clinical, ethyl alcoholfractionated dextrans (28).…”

Section: Ditionsmentioning

confidence: 89%

“…Dextran A, carefully hydrolyzed and converted to clinical dextrans with acid and then fractionated with ethyl alcohol, showed differences between number and weightaverage molecular weights, thus demonstrating heterogeneity (28). Furthermore, a light-scattering technique applied to various fractions of a native dextran gave a wide range of D P values (1) from 7.8 to 370 ( X lo4) , demonstrating heterogeneity.…”

Section: Experiments a N D Discussionmentioning

confidence: 99%

Reaction of Sodium Chlorite with Various Polysaccharides. Rate Studies and Aldehyde Group Determinations

Launer¹,

Tomimatsu²

1959

Anal. Chem.

View full text Add to dashboard Cite

Aldehyde contents and degree of polymerization values of polysaccharides were calculated from chlorite results obtained under various conditions and using the corresponding stoichiometric ratios determined for substances of low molecular weight. The polysaccharides were three native dextrans, a series of hydrolyzed dextrans including clinical dextran, a periodate dextran, a series of araban fractions, and starches including pea, wheat, and rice amylopectin, and a series of periodate cornstarch products. The results with various chlorite procedures were self-consistent and agreed well with those of other chemical methods, and fairly well with those of light-scattering for fractionated polymers. For native dextrans, where no agreement was to b e expected, the large divergence demonstrated inhomogeneity of substance. Agreement with osmotic pressure results was fair to poor. HE oxidation by sodium chlorite in T phosphate buffer of a variety of mono-and disaccharides and benzaldehyde was recently studied (12) with respect to the chlorite-aldehyde stoichiometry as affected by buffer strength, reaction rate, and concentration of reactants. Various experimental conditions employing sodium chlorite were dweloped for application t o the determination of aldehyde groups in polysaccharides.This communication describes the study of the rates of oxidation under those conditions and others of a series of periodate cornstarches; of a series of dextrans, including native, periodate, clinical, and more extensively acidhydrolyzed; and of a series of arabans.

show abstract

“…For clinical and technical dextran, the partial hydrolysis and further fractionation of native dextran gives products with the desired molecular sizes [79,80]. Variation of the concentration of the hydrochloric or sulfuric acid, the time and temperature of hydrolysis, and the nature and ratio of the precipitant (alcohol/water) for phase separation permits the control of the resultant average molecular weights.…”

Section: Industrial Productionmentioning

confidence: 99%

Functional Polymers Based on Dextran

Heinze

Liebert

Heublein

et al.

Advances in Polymer Science

253

198

View full text Add to dashboard Cite

Dextran, comprising a family of neutral polysaccharides consisting of an α-(1→6) linked d-glucose main chain with varying proportions of linkages and branches, depending on the bacteria used, is an interesting starting material for chemical modification reactions for the design of new functional polymers with promising properties. The review summarises recent results on structure characterisation of dextran including some comments on biosynthesis of this important class of biopolymers. Applications of dextran are discussed as well. Chemical modification reactions of dextran are increasingly studied for the structure and hence property design. The review highlights recent progress in esterification of dextran, both inorganic and organic polysaccharide esters, etherification reactions towards ionic and non-ionic ethers, and the huge variety of different conversions mainly developed for the binding of drugs. It summarises recent developments in the application of dextran derivatives with a focus on the chemical structures behind these materials such as prodrugs, bioactivity of inorganic dextran esters, heparin sulfate mimics, hydrogels, nanoparticles and self assembly structures for surface modification. Keywords Functionalised dextran • Structural analysis • Prodrugs • Nanostructures • Bioactivity Abbreviations AFM Atomic force microscopy AGU Anhydroglucose units AT Antithrombin AZT Azidothymidine BSH Na 2 B 12 H 11 SH BSA Bovine serum albumin CAC Critical association concentration CDI N,N -Carbonyldiimidazole CMC Critical micelle concentration CMD Carboxymethyl dextran CMDB Carboxymethyl dextran benzylamide CMDBSSu Carboxymethyl dextran benzylamidesulfonate sulfate CMDBSu Carboxymethyl dextran benzylamide sulfate CMDEE Carboxymethyl dextran ethyl ester CMDSu Carboxymethyl dextran sulfate COSY Two-dimensional correlated spectroscopy CsA Cyclosporin A 2D Two-dimensional Dach-Pt cis-Dihydroxo(cyclohexane-trans-l-1,2-diamine)platinum II DCC N,N -Dicyclohexylcarbodiimide T. Heinze et al.

show abstract

Production of Clinical-Type Dextran - Partial Hydrolytic Depolymerization and Fractionation of the Dextran from Leuconostoc mesenteroides Strain NRRL B-512

Cited by 31 publications

References 9 publications

Dextran: Sources, Structures, and Properties

Dextran: Sources, Structures, and Properties

Reaction of Sodium Chlorite with Various Polysaccharides. Rate Studies and Aldehyde Group Determinations

Functional Polymers Based on Dextran

Contact Info

Product

Resources

About