Production of cocktail of polyclonal antibodies using bacterial expressed recombinant protein for multiple virus detection

Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective

“…These recombinant proteins are used successfully for polyclonal antibodies (PAbs) production against viruses infecting papaya, bananas, and grapes and to develop immunodiagnostics for routine testing [256][257][258]. In this regard, an important advance was made by producing cocktails of PAbs against the recombinant fusion CP of two and three viruses infecting vegetable crops [259]. Dual and triple cocktails of PAbs were generated for DAC-ELISA toward the detection of CMV and papaya ringspot virus (PRSV; Potyvirus, Potyviridae) or CMV, PRSV, and groundnut bud necrosis virus (GBNV; Orthotospovirus, Tospoviridae) simultaneously in Cucurbitaceae, Solanaceae, and other hosts [259].…”

The Comparison of Antibodies Raised Against PLRV with Two Different Approaches - Viral Particles Purification and Recombinant Production of CP

“…Therefore, polyclonal antibodies raised against purified virions contaminated with host tissue components, cross react with host antigens and often give variable background reactions, thus limiting their use in ELISA based diagnostic methods. Recombinant polyclonal antibodies (Pab) specific to Tomato spotted wilt virus (TSWV) [11], Potato virus Y [12], Alfalfa mosaic virus (AMV) Khatabi et al [13] have been generated and used for serological detection of cited viruses [14]. centrifuged at 12.000 rpm for 15 min.…”