Production of concentrated suspensions of thermophilic lactic acid bacteria in calcium-alginate beads

Enhancement of freezing-resistance of Lactobacillus rhamnosus by the application of cell immobilization

“…After defrosting, the samples were cultured on MRS agar and the standard plate count method (SPC method) was used to determine the viable cell number (Speck, 1984). For the determination of viable lactic acid bacteria number, Ca-alginate entrapped samples were dissolved in 4% EDTA (ethylenediaminetetraacetic acid) solution (Champagne et al, 1993(Champagne et al, , 1996Kearney et al, 1990), and physiological saline was used for the dissolution of k-carrageenan gel beads (King and Zall, 1983b).…”

Continuous mixed strain mesophilic lactic starter production in supplemented whey permeate medium using immobilized cell technology

“…9). Champagne et al (1993a) have shown that pH, in the range 5.2 to 6.1, affected both the populations of Streptococcus salivarius subsp. thermophilus and Lactobacillus delbrueeckii subsp.…”

Fermentation technologies for the production of exopolysaccharide-synthesizing Lactobacillus rhamnosus concentrated cultures

“…The production of biomass by growing lactic cultures in alginate beads had previously been suggested (Champagne et al 1992;Champagne et al 1993), particularly for cultures which were sensitive to oxygen or centrifugation stresses. For EPS producers, the immobilization techniques may facilitate the recovery of the cells even in a viscous environment as they are entrapped in beads (Table 2) and this presents the main advantage.…”

“…Although the production of concentrates in alginate beads has the advantage of eliminating the need for a concentration step, the EPS yields are sometimes lower (Champagne et al 1993) and some strains are adversely affected by entrapment in alginate gels (Lamboley et al 2003). No data are available on the ICT production of EPS-producing strains of L. rhamnosus.…”