Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1: media optimization using experimental design

Mahat, Mohd Khairizal; Illias, Rosli Md.; Rahman, Roshanida A.; Rashid, Noor Aini Abd; Mahmood, Nik Hasnaa Nik; Hassan, Osman; Aziz, Suraini Abdul; Kamaruddin, Kamarulzaman

doi:10.1016/j.enzmictec.2004.07.008

Cited by 65 publications

(36 citation statements)

References 21 publications

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“…As stated previously, soluble starch and yeast extract have induction effects on CGTase production. [30,31] In contrast, the negative coefficient of temperature (¡1.14) suggested that low temperature is more favourable for CGTase biosynthesis. Therefore, the three non-significant factors were eliminated from the formula and a new equation was formed as follows:…”

Section: Placketàburman Designmentioning

confidence: 88%

“…It seems that in the mentioned cases, recombinant CGTase production, specially using the NICE system, requires high nitrogen content to achieve its maximum expression capacity. The inducer-like activities of yeast extract toward CGTase production [31] might be the reason for its high optimal concentration.…”

Section: Artificial Neural Networkmentioning

confidence: 99%

“…Normally, however, the optimum temperature for various producers of CGTase and for L. lactis producing different products is higher than 25 C. For example, Low et al [42] reported 34.7 C as the optimum temperature for CGTase biosynthesis by E. coli. Rosso et al [43], Mahat et al [31] and Higuti et al [44] concluded that 37 C was the optimum temperature for maximum CGTase production by various Bacillus strains. In the case of L. lactis, Mall et al [45] and Sharma et al [46] determined 30 C and 35 C as the best temperature to achieve maximum nisin and bacteriocin production, respectively.…”

Section: Artificial Neural Networkmentioning

confidence: 99%

“…As mentioned previously, yeast extract is a suitable nitrogen source for CGTase production by different species. Mahat et al [31] studied the optimum yeast extract concentration for CGTase production by Bacillus sp. TS1-1 and found the best concentration to be 1.89% (w/v).…”

Section: Artificial Neural Networkmentioning

confidence: 99%

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Cyclodextrin glycosyltransferase biosynthesis improvement by recombinantLactococcus lactisNZ:NSP:CGT: medium formulation and culture condition optimization

Amiri

Mohamad

Rahim

et al. 2015

Biotechnology & Biotechnological Equipment

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Cyclodextrin glycosyltransferase (CGTase) is a bacterial glycosyltransferase which catalyses the conversion of starch to cyclodextrin. It can be produced industrially through a fermentation process. The optimal design of the fermentation medium and conditions is critical for metabolic production and microbial growth. Optimization of CGTase biosynthesis by recombinant Lactococcus lactis NZ:NSP:CGT was performed using an artificial neural network (ANN) as the main tool in order to improve the CGTase production in the fermentation process. Three parameters, including temperature, carbon source concentration and nitrogen source concentration, were determined as significant factors for improvement of CGTase production by L. lactis NZ:NSP:CGT in the fermentation process. Soluble potato starch and yeast extract were chosen as the best carbon and nitrogen sources for higher production of CGTase by L. lactis NZ:NSP:CGT. The optimum concentration of soluble starch and yeast extract were 3.82% and 5.67% (w/v), respectively, and the optimum temperature was 20C. The final CGTase activity under optimized conditions was 22.09 U/mL, which was close enough to the predicted value of 24.17 U/mL. The obtained results are of particular interest, as CGTase is an industrially important enzyme. In this study, its production through batch fermentation was optimized in order to maximize its production. In future studies, more investigations in various modes of fermentation should be conducted in order to increase the CGTase biosynthesis.

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Section: Placketàburman Designmentioning

confidence: 88%

Section: Artificial Neural Networkmentioning

confidence: 99%

Section: Artificial Neural Networkmentioning

confidence: 99%

Section: Artificial Neural Networkmentioning

confidence: 99%

See 2 more Smart Citations

Cyclodextrin glycosyltransferase biosynthesis improvement by recombinantLactococcus lactisNZ:NSP:CGT: medium formulation and culture condition optimization

Amiri

Mohamad

Rahim

et al. 2015

Biotechnology & Biotechnological Equipment

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“…Literature suggests that the experimental results can be greatly enhanced with the help of RSM compared to the conventional optimization methods (Mahat et al, 2004). Using RSM, Mahat et al, (2004) and Tanyildizi et al, (2006) reported an increase in the production yields of cyclodextrin glucanotransferase (CGTase) and α-amylase by 34% and 15%, respectively.…”

Section: Statistical Analysis Of Enzymatic Hydrolysismentioning

confidence: 99%

Optimization of Enzymatic Hydrolysis of Corn Stover for Improved Ethanol Production

Zambare¹,

Christopher

2012

Energy Exploration & Exploitation

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Response surface methodology (RSM) was used to optimize the enzymatic hydrolysis of corn stover (CS), an abundant agricultural residue in the USA. A five-level, three-variable central composite design (CCD) was employed in a total of 20 experiments to model and evaluate the impact of pH (4.1-6.0), solids loadings (6.6-23.4%), and enzyme loadings (6.6−23.4 FPU g −1 DM) on glucose yield from thermo-mechanically extruded CS. The extruded CS was first hydrolyzed with the crude cellulase of Penicillium pinophilum ATCC 200401 and then fermented to ethanol with Saccharomyces cerevisiae ATCC 24860. Although all three variables had a significant impact, the enzyme loadings proved the most significant parameter for maximizing the glucose yield. A partial cubic equation could accurately model the response surface of enzymatic hydrolysis as the analysis of variance (ANOVA) showed a coefficient of determination (R 2 ) of 0.82. At the optimal conditions of pH of 4.5, solids loadings of 10% and enzyme loadings of 20 FPU g −1 DM, the enzymatic hydrolysis of pretreated CS produced a glucose yield of 57.6% of the glucose maximum yield which was an increase of 10.4% over the non-optimized controls at zero-level central points. The predicted results based on the RSM regression model were in good agreement with the actual experimental values. The model can present a rapid means for estimating lignocellulose conversion yields within the selected ranges.

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Optimization of the Reaction Parameters of Immobilized Escherichia coli for β‐Cyclodextrin Production

et al. 2023

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Cyclodextrin (CD) is an important substance for chemical, pharmaceutical, and food industries. Conventionally, free Escherichia coli (E. coli) cells expressing recombinant cyclodextrin glucanotransferase (CGTase) are used as the biocatalyst for CD production, but the process struggles with low CGTase excretion and CD yield. In this study, E. coli cells were immobilized on hollow‐fiber membranes for β‐CD production, and the process and reaction parameters were optimized via response surface methodology. The reusability of the immobilized cells was also evaluated. The parameter optimization significantly improved β‐CD yield and CGTase excretion, making the process more attractive for industrial applications. The immobilized cells also revealed to be reusable multiple times.

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Production of cyclodextrin glucanotransferase (CGTase) from alkalophilic Bacillus sp. TS1-1: media optimization using experimental design

Cited by 65 publications

References 21 publications

Cyclodextrin glycosyltransferase biosynthesis improvement by recombinantLactococcus lactisNZ:NSP:CGT: medium formulation and culture condition optimization

Cyclodextrin glycosyltransferase biosynthesis improvement by recombinantLactococcus lactisNZ:NSP:CGT: medium formulation and culture condition optimization

Optimization of Enzymatic Hydrolysis of Corn Stover for Improved Ethanol Production

Optimization of the Reaction Parameters of Immobilized Escherichia coli for β‐Cyclodextrin Production

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