Production of cyclodextrin glucanotransferase from an alkalophilic Bacillus sp. by pH-stat fed-batch fermentation

Abstract

Batch and fed-batch fermentation processes were employed to culture an alkalophilic Bacillus sp. for the production of cyclodextrin glucanotransferase (CGTase). CGTase production was repressed by glucose and induced by soluble starch. By fed-batch fermentation, a CGTase activity up to 56 unit ml(-1) with 65 g dry cells l(-1) were achieved. The CGTase activity and cell density were increased 360 and 510%, respectively, from those values achieved with batch fermentation.

Excretory overexpression of Paenibacillus pabuli US132 cyclodextrin glucanotransferase (CGTase) in Escherichia coli: gene cloning and optimization of the culture conditions using experimental design

“…The major activities of these enzymes are intramolecular (cyclization) and intermolecular (disproportion, coupling) transglycosylation (Kuo et al 2009). They are able to convert starch into cyclic α-1,4-glucans, called cyclodextrins (CDs) (Leemhuis et al 2010).…”

Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

“…They carried experiments in batch without aeration, reaching a CGTase activity of approximately 0.15 U/mL at 72 hours of fermentation. Kuo [35] studied CGTase production in batch with non-immobilized Bacillus sp.…”

Cyclodextrin glycosyltransferase biosynthesis improvement by recombinantLactococcus lactisNZ:NSP:CGT: medium formulation and culture condition optimization

“…[47] Nisin induction is capable of acting in a vast range of temperatures, e.g. as low as 20 C, but low temperatures might reduce the induction and heterologous protein production. [40] Cultivating the recombinant bacteria at low temperatures enhances the desirable heterologous protein production.…”

“…[18,19] There are many studies by a number of researchers, e.g. Ravinder et al [19], Ahmed and El-Refai [7], Kuo et al [20], Menocci and Goulart [21] and Illias et al [10], conducted for the purpose of fermentation optimization, which reflects the importance of this issue. According to Khurana et al [22], the univariate technique is not suitable for use in all cases of optimized factor level determination in the enzyme production process.…”