Production of Cyclodextrins by CGTase from Bacillus clausii Using Different Starches as Substrates

Alves-Prado, Heloíza Ferreira; Carneiro, A. A. J.; Pavezzi, Fabiana Carina; Gomes, Eleni; Boscolo, Maurício; Franco, Célia Maria Landi; Silva, R. da

doi:10.1007/978-1-60327-526-2_15

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…The relative quantities of the formed ␥-CDs were high compared to the yields of 13.9% ␥-CD attained with purified enzyme from alkaliphilic Bacillus sp. 277 [12] after 12 h reaction at 60 • C, pH 8.0 with 400 U enzyme per gram substrate or compared to 11% ␥-CD achieved by a purified CGTase from alkaliphilic Bacillus clausii E16 after 24 h reaction at 55 • C [6]. Both biocatalysts led to an appreciable increased total CD content after 24 h enzyme reac- tion with a dominant presence of ␤-CD (85%).…”

Section: Cyclodextrin Production By Cell Membrane-biocatalysts As Enzmentioning

confidence: 96%

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Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli

Atanasova

Kitayska

Yankov

et al. 2009

Biochemical Engineering Journal

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Section: Cyclodextrin Production By Cell Membrane-biocatalysts As Enzmentioning

confidence: 96%

“…CGTase is mainly produced by members of the genus Bacillus. Recently, the alkaliphilic bacilli rouse a big interest on account of their valuable and commercially important CGTases possessing a significant activity over a wide range of pH and relatively high temperature [4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli

Atanasova

Kitayska

Yankov

et al. 2009

Biochemical Engineering Journal

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“…• Liquefied starch, maltodextrin, or long-chain maltooligosaccharides (Rendleman 1999) • Maltodextrin and commercial soluble starch, corn, cassava, sweet potato, and waxy corn starches (Alves-Prado et al 2008) • Amaranth starch (Urban et al 2012) • Soluble and cocoyam starch (Mora et al 2012) • Starch such as potato, tapioca, and corn (Shahrazi et al 2013) CGTase is specifically active on both structures of amylose and amylopectin (Shahrazi et al 2013). Several microorganisms and methods have been used to produce or use CGTase as described in the following: Fig.…”

Section: Occurrence/sourcesmentioning

confidence: 99%

Cyclodextrins

Couto¹,

Salústio²,

Cabral-Marques³

2014

Polysaccharides

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Cyclodextrins (CDs) were first isolated in 1891 as degradation products of starch from a medium of Bacillus amylobacter. They are cyclic water-soluble, nonreducing, macrocycle carbohydrate polymers constructed from a-(1-4)-linked D-glucopyranose units (naturally occurring a, b, and g formed by 6, 7, and 8 glucose units), in a ring formation and present a toroidal, hollow, truncated cone shape. Their most important property is the ability to establish specific interactions -molecular encapsulation -with various types of molecules through the formation of non-covalently bonded entities, either in the solid phase or in aqueous solution, taking up a whole molecule, or some part of it, into their cavities. This process in part mimics the "lock and key" mechanism of enzyme catalysis. Complexation may cause changes in physicochemical properties of the guest molecule (e.g., solubility, stability, kinetics and bioavailability, toxicity). Their negligible cytotoxic effects promoted them to the GRAS list and led them to be widely used in many industrial products and technologies.

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“…the family GH13 of glycoside hydrolases, constitutes an important studied group of the starch-degrading enzymes (Van der Veen et al 2000;Alves-Prado et al 2008). Enzymes belonging to this family have three carboxylic residues (2 aspartates and 1 glutamate) implicated in the catalysis and employ an α-retaining double displacement mechanism.…”

Section: Introductionmentioning

confidence: 99%

Improvement of cyclodextrin glycosyltransferase (CGTase) production by recombinant Escherichia coli pAD26 immobilized on the cotton

et al. 2012

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Abstract:The cyclodextrin glycosyltransferase (CGTase) of the recombinants Escherichia coli pAD26 cells immobilized on cotton was optimally produced by statistical methodology. Primarily, carbon and nitrogen sources were selected by one-factor-at-a-time method. Wheat starch, Casamino acid, Edamin and Hy-soy were identified as the best nutrients. These sources were secondly confirmed by Plackett-Burman design (fifteen variables were studied with sixteen experiments), as the most significant components with respect to CGTase production. In the third step, concentration of most significant factors and their interaction were optimized with a Box-Behnken experimental design. Under the optimized conditions (agitation 200 rpm, yeast extract concentration 20 g/L, wheat starch concentration 10 g/L and Hy-soy concentration 2.5 g/L), CGTase yield 145.11 U/mL was 3.6 and 23 folds higher than those obtained by the use of the initial conditions (39.77 U/mL) and free cells (6.37 U/mL), respectively.

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Production of Cyclodextrins by CGTase from Bacillus clausii Using Different Starches as Substrates

Cited by 8 publications

References 25 publications

Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli

Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli

Cyclodextrins

Improvement of cyclodextrin glycosyltransferase (CGTase) production by recombinant Escherichia coli pAD26 immobilized on the cotton

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