Production of d -ribose by metabolically engineered Escherichia coli

Park, Hae-Chul; Kim, Hee‐Tak; Lee, Chang-Wan; Rho, Yong-Taek; Kang, JeongWoo; Lee, Dae-Hee; Seong, Yeong-Je; Park, Yong-Cheol; Lee, Dae-Sang; Kim, Sung-Gun

doi:10.1016/j.procbio.2016.10.001

Cited by 12 publications

(12 citation statements)

References 21 publications

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“…The recombinant and WT strains were individually cultured in the medium containing 5 g/L xylose and 5 g/L glucose reported by Park et al [30] . in a shake flask for comparisons.…”

Section: Resultsmentioning

confidence: 99%

“…in a shake flask for comparisons. Because the recombinant strain is transketolase‐deficient and carbon catabolite repression‐negative, the addition of glucose was reported to improve its growth [30] . However, the growth rate of recombinant strain was still slower than the WT strain according to OD600 measurement (Figure S9).…”

Section: Resultsmentioning

confidence: 99%

“…E. coli MG1655 ~tktA ~tktB ~ptsG, which can metabolize xylose to generate Dribose, was constructed. [30] After 48 h cultured in the medium containing 5 g/L xylose and 5 g/L glucose reported by Park et al [30] in a shake flask, 2.20 g/L D-ribose, which is 14.7 mM, was generated in the supernatant. Finally, the supernatant was successfully applied to make the final concentration of 1 mM Dribose in 10 mL optimal biocascade reaction to generate 0.84 mM NMN, which is 84 % yield, in 75 min.…”

Section: Introductionmentioning

confidence: 92%

“…Kim et al constructed a metabolically engineered E. coli SGK015 with the knockout of tktA, tktB, and ptsG genes to metabolize xylose to generate 3.75 g/L D-ribose (25 mM) in the batch fermentation. [30] The tktA and tktB genes encode two transketolase enzymes in the pentose phosphate pathway. E. coli mutant with knockout of tktA and tktB generated D-ribose from xylose after glucose depletion in the media.…”

Section: D-ribose Production From Xylose By E Coli Mg1655 δTkta δTktb...mentioning

confidence: 99%

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Synthesis of Nicotinamide Mononucleotide from Xylose via Coupling Engineered Escherichia coli and a Biocatalytic Cascade

et al. 2022

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β‐Nicotinamide mononucleotide (NMN) has recently gained attention for a nutritional supplement because it is an intermediate in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). In this study, we developed NMN synthesis by coupling two modules. The first module is to culture E. coli MG1655 ▵tktA ▵tktB ▵ptsG to metabolize xylose to generate D‐ribose in the medium. The supernatant containing D‐ribose was applied in the second module which is composed of EcRbsK‐EcPRPS‐CpNAMPT reaction to synthesize NMN, that requires additional enzymes of CHU0107 and EcPPase to remove feedback inhibitors ADP and pyrophosphate. The second module can be rapidly optimized by comparing NMN production determined by the cyanide assay. Finally, 10 mL optimal biocascade reaction generated NMN with a good yield of 84 % from 1 mM D‐ribose supplied from the supernatant of E. coli MG1655 ▵tktA ▵tktB ▵ptsG. Our results can further guide researchers to metabolically engineer E. coli for NMN synthesis.

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“…The recombinant and WT strains were individually cultured in the medium containing 5 g/L xylose and 5 g/L glucose reported by Park et al [30] . in a shake flask for comparisons.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: D-ribose Production From Xylose By E Coli Mg1655 δTkta δTktb...mentioning

confidence: 99%

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Synthesis of Nicotinamide Mononucleotide from Xylose via Coupling Engineered Escherichia coli and a Biocatalytic Cascade

et al. 2022

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“…As described in previous studies [ 47 – 50 ], we determined the molecular weight distribution and the nucleotide and ribose contents of Deproteinized Calf Blood Extractive Injection (DCBEI)(CAS: 20160803; obtained from Jilin Connell Pharmaceutical Co. LTD., Jilin, China) samples using high-performance liquid chromatography (HPLC) (E2695, Waters Co., Ltd., USA) systems and gel permeation chromatography (1515, Waters Co., Ltd., USA).…”

Section: Methodsmentioning

confidence: 99%

The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling

Wang

Chen

et al. 2021

Aging

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Alzheimer’s disease (AD) is characterized by cognitive decline due to the accumulation of extracellular β-amyloid (Aβ) plaques and neurofibrillary tangles in the brain, which impair glutamate (Glu) metabolism. Deproteinized Calf Blood Extractive Injection (DCBEI) is a biopharmaceutical that contains 17 types of amino acids and 5 types of nucleotides. In this study, we found that DCBEI pretreatment reduced L-Glu-dependent neuroexcitation toxicity by maintaining normal mitochondrial function in HT22 cells. DCBEI treatment also reduced the expression of pro-apoptosis proteins and increased the expression of anti-apoptosis proteins. Furthermore, DCBEI attenuated AD-like behaviors (detected via the Morris water maze test) in B6C3-Tg (APPswePSEN1dE9)/Nju double transgenic (APP/PS1) mice; this effect was associated with a reduction in the amount of Aβ and neurofibrillary tangle deposition and the concomitant reduction of phospho-Tau in the hippocampus. Metabonomic profiling revealed that DCBEI regulated the level of neurotransmitters in the hippocampus of APP/PS1 mice. Label-free proteomics revealed that DCBEI regulated the expression of Nrf-2 and its downstream targets, as well as the levels of phospho-protein kinase B and mitogen-activated protein kinase. Together, these data show that DCBEI can ameliorate AD symptoms by upregulating Nrf2-mediated antioxidative pathways and thus preventing mitochondrial apoptosis.

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Enhanced D-ribose production by genetic modification and medium optimization in Bacillus subtilis 168

Zhao

Liu

Zhang

et al. 2018

Korean J. Chem. Eng.

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Production of d -ribose by metabolically engineered Escherichia coli

Cited by 12 publications

References 21 publications

Synthesis of Nicotinamide Mononucleotide from Xylose via Coupling Engineered Escherichia coli and a Biocatalytic Cascade

Synthesis of Nicotinamide Mononucleotide from Xylose via Coupling Engineered Escherichia coli and a Biocatalytic Cascade

The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling

Enhanced D-ribose production by genetic modification and medium optimization in Bacillus subtilis 168

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