Production of Diphtheria Toxin in Submerged Culture

The amino acid requirements of Corynebacterium diphtheriae PW 8 substrain CN 2000

“…The complex medium (Linggood and Fenton 1947;Linggood et al 1954Linggood et al , 1955Edwards 1960;Stainer 1967a;Righelato and Hemert 1969;Hemert 1974;Thomas 1979) used in fermentation contains beef enzymatic hydrolysate which gives high toxin yields. Although problems concerning the use of beef hydrolysate-based media have been pointed out (Stainer 1967a, b;Stainer and Scholte 1973;Cox and Hardegree 1976) and some of the vaccines manufactured using beef media have been shown to contain bovine antigens (Stainer 1967a;Cox and Hardegree 1976), major complications due to beef-based vaccines have not been reported (Cox and Hardegree 1976).…”

The Keto Acid Metabolism of Corynebacterium diphtheriae growing in Submerged Culture

“…This seemed to be one of the few if not the only instance of a direct relationship between toxin production and a metabolite which had been reported for this organism, and it was decided to investigate the keto acid metabolism of various strains of C. diphtheriae growing in a stirred and aerated culture of the type described by Linggood, Matthews, Pinfield, Pope & Sharland (1955 …”