Production of Dothiepin sulfoxide Carbonyl compounds by the phage resistant Streptomyces coeruleorubidus.

Abstract

Researchers are increasingly looking for novel antibiotics due to the lack of new methicillin-resistant antibiotics against staphylococcus aureus (MRSA) and anti-Vancomycin-resistant Enterococci (VRE) matter. Streptomyces is the source of 66% of used antimicrobial substances. Moreover, improving phage-resistant strains of Streptomyces disposed of the phages and significantly increased the production of antimicrobial substances. In this study, 58 isolates of Actinobacteria were isolated from seven soil samples of rhizospheric soil in Egypt and tested for antimicrobial activity. A Streptomyces coeruleorubidus strain showed a strong antimicrobial effect on five groups of Gram +ve and Gram –ve microorganisms are known to be potential human pathogens. Colonies of S. coeruleorubidus were selected and identified following the International Streptomyces Project (ISP) method and the 16S rRNA sequence appeared to be 96.47–100% similar to various Streptomyces spp. The metabolite produced was identified as Dothiepin sulfoxide with advanced spectroscopy Nuclear magnetic resonance (NMR) spectroscopy, and gas chromatography-mass spectrometry instrument(GC mass). Unfortunately, a lysis of the production strain was observed. Three Streptomyces phages (ϕPRSC1, ϕPRSC2, and ϕPRSC4) were isolated and characterized using S. coeruleorubidus as a bacteria indicator. DNA of phage was digested with two restriction enzymes, EcoRI and HindIII. DNA of the phage ϕPRSC2 is sensitive to HindIII and fragments into two different banding patterns, whereas DNA of ϕPRSC1 and ϕPRSC4 is resistant to both enzymes. The phage-resistant bacterial strain was more stable than the original sensitive strain. This study suggested using phage-resistant strains of S. coeruleorubidus to produce the antimicrobial metabolite Thiocarbamic acid, N,N-dimethyl, -1,3-diphenyl-2-butenyl ester (Dothiepin sulfoxide). While screening for new antibiotics produced by actinomycetes, colonies that do not appear lysed must be selected.