Production of doubled haploid plants of carnation (Dianthus caryophyllus L.) by pseudofertilized ovule culture

Sato, Sunao; Katoh, Norio; Yoshida, Hiroyuki; Iwai, Sumio; Hagimori, Manabu

doi:10.1016/s0304-4238(99)00090-4

Cited by 38 publications

(26 citation statements)

References 9 publications

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“…It is well known in barley (H. vulgare) that haploidy is efficiently produced by crossing with Hordeum bulbosum, a wild relative species, due to the selective elimination of H. bulbosum chromosomes at the early stage of embryo development (Kasha and Kao 1970). Furthermore, there is a report in carnation that doubled-haploids were yielded by inducing pseudogamy by pollinating with X-ray irradiated pollen (Sato et al 2000). These results may suggest that the carnation-like progenies obtained in the present study are doubled-haploids.…”

Section: Maternal Plant-like Progenies Having No Evidence Of Hybriditymentioning

confidence: 99%

Unilateral compatibility and genotypic difference in crossability in interspecific hybridization between Dianthus caryophyllus L. and Dianthus japonicus Thunb

Nimura¹,

Kato

Mii

et al. 2003

Theor Appl Genet

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Reciprocal interspecific crosses were carried out between six lines of Dianthus caryophyllus L. and one line of Dianthus japonicus Thunb. Although no seed was set when D. japonicus was used as the seed parent, six seedlings were successfully obtained from 2,380 immature ovules by applying the embryo-rescue technique. However, they showed seed parent-like morphology and no evidence for the hybridity by flow cytometry and RAPD analyses. When six lines of D. caryophyllus were used as seed parents, a total of 192 seedlings were successfully obtained without using the embryo-rescue technique. Among these seedlings, 12 out of 25 progenies obtained from the carnation line '98sp1651' were confirmed to be the hybrids. The remaining 13 progenies of this line, and the total 167 progenies obtained from the other carnation lines, had carnation-like morphology without any evidence of hybridity by flow cytometry and RAPD analyses. The progenies confirmed as hybrids had intermediate characters of the parents with respect to leaf width and flower size, but they had a uniform flower color, reddish purple, which was different from that of either parent. Since the hybrids obtained in the present study have some profitable characters such as vigorous growth in summer time, upright robust stem, broad leaves and early flowering, they are expected to be used for the breeding of carnation which is suitable for growing under the Japanese climate.

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Section: Maternal Plant-like Progenies Having No Evidence Of Hybriditymentioning

confidence: 99%

Unilateral compatibility and genotypic difference in crossability in interspecific hybridization between Dianthus caryophyllus L. and Dianthus japonicus Thunb

Nimura¹,

Kato

Mii

et al. 2003

Theor Appl Genet

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show abstract

“…Since the carnation cultivars are highly heterozygous due to inbreeding depression (Sato et al 2000), most of the commercially important cultivars are clones of selected individuals that have been propagated vegetatively. Hence, neither pure-bred varieties nor F 1 hybrids have been produced.…”

Section: Introductionmentioning

confidence: 99%

Induction of Fertile Amphidiploids by Artificial Chromosome-doubling in Interspecific Hybrid between Dianthus caryophyllus L. and D. japonicus Thunb

et al. 2006

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Amphidiploid induction by chromosome-doubling is the most efficient method of utilizing a sterile interspecific hybrid for breeding. Previously, although we successfully obtained an interspecific hybrid between carnation (Dianthus caryophyllus L.) and Dianthus japonicus Thunb., the hybrid was sterile. In the present study, therefore, attempts were mode to induce amphidiploids to restore the fertility by artificial chromosomedoubling, i.e., by applying drops of a colchicine solution on to the shoot tips of greenhouse-grown plants or by treating nodal segments excised from in vitro-grown plants by incubation in a colchicine or amiprophosmethyl (APM) solution. Among 156 hybrid plants regenerated after these treatments, nine were revealed to be tetraploids, in addition to one octoploid and 88 mixoploids. Colchicine dropping treatment at a concentration of 2,000 mg⋅l −1 for one day led to the highest rate (14%) of tetraploid formation, whereas 10% tetraploid formation was obtained by in vitro shaking culture with 5 and 10 mg⋅l −1 APM for 24 hrs. The amphidiploids exhibited a larger flower size and later flowering time than the original diploid hybrid, and the fertility of both pollen and seed was restored. These amphidiploids are expected to be used for the breeding of carnation cultivars, which are suitable for growing under the Japanese climatic conditions.

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“…Carnation cultivars are developed to be highly heterozygous so as to avoid the effects of inbreeding depression. Sato et al (2000) reported that inbreeding depression of carnation appears in the third selfed generation (S 3 ), so it is almost impossible to produce S 4 seeds.…”

Section: Discussionmentioning

confidence: 99%

Effects of Crossing and Selection for Seven Generations Based on Flower Vase Life in Carnations (<i>Dianthus caryophyllus</i> L.), and the Relationship between Ethylene Production and Flower Vase Life in the Breeding Lines

et al. 2018

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Production of doubled haploid plants of carnation (Dianthus caryophyllus L.) by pseudofertilized ovule culture

Cited by 38 publications

References 9 publications

Unilateral compatibility and genotypic difference in crossability in interspecific hybridization between Dianthus caryophyllus L. and Dianthus japonicus Thunb

Unilateral compatibility and genotypic difference in crossability in interspecific hybridization between Dianthus caryophyllus L. and Dianthus japonicus Thunb

Induction of Fertile Amphidiploids by Artificial Chromosome-doubling in Interspecific Hybrid between Dianthus caryophyllus L. and D. japonicus Thunb

Effects of Crossing and Selection for Seven Generations Based on Flower Vase Life in Carnations (<i>Dianthus caryophyllus</i> L.), and the Relationship between Ethylene Production and Flower Vase Life in the Breeding Lines

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