Production of doubled haploid plants in cucumber (Cucumis sativus L.) via parthenogenesis

Salehian, Hamed; Shahnazi, S; Nazari, Mohamadreza

doi:10.1007/s11627-023-10368-y

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2024

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Cited by 4 publications

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Haploid regeneration system suitable for various genotypes of thin-skinned melons

Li,

Hou,

Luo

et al. 2024

Plant Cell Tiss Organ Cult

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Haploid regeneration system suitable for various genotypes of thin-skinned melons

Li,

Hou,

Luo

et al. 2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Production of haploid plants in wax gourd through parthenogenesis induced by gamma-irradiated pollen

Liu,

Xie,

Xiao

et al. 2024

Scientia Horticulturae

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Haploid Regeneration System Suitable for Various Genotypes of Thin-Skinned Melons

Li,

Hou,

Luo

et al. 2024

Preprint

View full text Add to dashboard Cite

Cucumis melo ssp. agrestis is a vital crop in the Cucurbitaceae family. Traditional breeding has been the primary method, but prolonged artificial selection has led to a gradual decline in the genetic diversity of thin-skinned sweet melon. An efficient induction system for thin-skinned sweet melon haploids has been established to innovate germplasm resources. Initially, unfertilized ovules treated with low temperature are peeled and inoculated onto the callus induction medium (CIM) for 2 days of dark culture, followed by 30 days of culture under subdued light to induce callus formation. Subsequently, the callus tissue is transferred to a proliferation medium (PM) and cultured under intense light until green cells and bud differentiation occur. Finally, rooting induction medium (RIM) induces rooting to form complete plants. Through validation with eight genotypes of disease-resistant thin-skinned sweet melon, this method exhibits a haploid induction rate of 12.26%, a diploid induction rate of 54.72%, and a tetraploid induction rate of 33.02%. Incorporating molecular marker-assisted breeding techniques, we have developed usable plants resistant to powdery mildew, downy mildew, and frost mold.

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Production of doubled haploid plants in cucumber (Cucumis sativus L.) via parthenogenesis

Cited by 4 publications

References 34 publications

Haploid regeneration system suitable for various genotypes of thin-skinned melons

Haploid regeneration system suitable for various genotypes of thin-skinned melons

Production of haploid plants in wax gourd through parthenogenesis induced by gamma-irradiated pollen

Haploid Regeneration System Suitable for Various Genotypes of Thin-Skinned Melons

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