Production of ectoine by <i>Halomonas elongata</i> BK-AG25 using osmotic shock technique

Parwata, I Putu; Wahyuningrum, Deana; Suhandono, Sony; Hertadi, Rukman

doi:10.1088/1755-1315/209/1/012017

Cited by 8 publications

(6 citation statements)

References 10 publications

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“…Ectoine is a compatible solute that serves as a protective substance by acting as an osmolyte and thus helps organisms survive extreme osmotic stress, for example, with high salt content [ 100 ]. Ectoine from Halomonas elongate extracted with low salt concentration solution is an adsorbent of ultraviolet light [ 101 , 102 ]. Mycosporine-like amino acids that also absorb ultraviolet light can be extracted from dry biomass employing methanol to assist the extraction and chloroform to eliminate pigments from Scytonema cf.…”

Section: Photoprotectorsmentioning

confidence: 99%

Microalgae Bioactive Compounds to Topical Applications Products—A Review

et al. 2022

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Microalgae are complex photosynthetic organisms found in marine and freshwater environments that produce valuable metabolites. Microalgae-derived metabolites have gained remarkable attention in different industrial biotechnological processes and pharmaceutical and cosmetic industries due to their multiple properties, including antioxidant, anti-aging, anti-cancer, phycoimmunomodulatory, anti-inflammatory, and antimicrobial activities. These properties are recognized as promising components for state-of-the-art cosmetics and cosmeceutical formulations. Efforts are being made to develop natural, non-toxic, and environmentally friendly products that replace synthetic products. This review summarizes some potential cosmeceutical applications of microalgae-derived biomolecules, their mechanisms of action, and extraction methods.

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Section: Photoprotectorsmentioning

confidence: 99%

Microalgae Bioactive Compounds to Topical Applications Products—A Review

et al. 2022

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“…As an initial step towards integrating eCO 2 RR to formate at high salinity and its interfacing with halophilic microbial catalysis using formate, the effect of MM63 V media composition on the electrochemical processes was studied. The MM63 V medium is thereby representative of a model halophilic growth medium, as it is used for various pure culture halophilic strains [39–43] …”

Section: Resultsmentioning

confidence: 99%

High Salt Electrolyte Solutions Challenge the Electrochemical CO₂Reduction Reaction to Formate at Indium and Tin Cathodes

Kas,

Izadi,

Harnisch

2023

ChemElectroChem

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Formate is a promising product of the electrochemical CO2 reduction reaction (eCO2RR) that can serve as feedstock for biological syntheses. Indium (In) has been shown as a selective electrocatalyst of eCO2RR with high coulombic efficiency (CE) for formate production at small scale at biocompatible non‐halophilic that is low salt conditions. Ohmic losses and challenges on potential/current distribution arise for scaling‐up, where higher salt loads are advantageous for minimizing these. Higher salt concentration within the solution or halophilic conditions also enable the use of halophilic biocatalysts. We optimized eCO2RR with halophilic media by introducing tin (Sn) as a more sustainable alternative to In. At 3 % NaCl providing a catholyte conductivity ( of 70 mS cm−1, the maximum specific formate production rates (rformate) of 0.143±0.030 mmol cm−2 h−1 and 0.167±0.027 mmol cm−2 h−1 were achieved at In and Sn electrocatalysts, respectively. Decrease in rformate and CE, in addition to higher variation between replicates was observed with further increase in NaCl concentration above 3 % ( >70 mS cm−1) up to 10 % ( =127 mS cm−1). This study sets the foundation for integrated microbial synthesis by halophiles.

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“…For extraction of ectoine a previously described method was used 80 . Briefly, ectoine was extracted from the cell pellet with methanol/chloroform/water (10/5/4 v/v/v) by vigorous shaking for 90 min.…”

Section: Methodsmentioning

confidence: 99%

Genome analysis of a halophilic bacterium Halomonas malpeensis YU-PRIM-29T reveals its exopolysaccharide and pigment producing capabilities

Athmika

Ghate

Arun

et al. 2021

Sci Rep

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Halomonas malpeensis strain YU-PRIM-29T is a yellow pigmented, exopolysaccharide (EPS) producing halophilic bacterium isolated from the coastal region. To understand the biosynthesis pathways involved in the EPS and pigment production, whole genome analysis was performed. The complete genome sequencing and the de novo assembly were carried out using Illumina sequencing and SPAdes genome assembler (ver 3.11.1) respectively followed by detailed genome annotation. The genome consists of 3,607,821 bp distributed in 18 contigs with 3337 protein coding genes and 53% of the annotated CDS are having putative functions. Gene annotation disclosed the presence of genes involved in ABC transporter-dependent pathway of EPS biosynthesis. As the ABC transporter-dependent pathway is also implicated in the capsular polysaccharide (CPS) biosynthesis, we employed extraction protocols for both EPS (from the culture supernatants) and CPS (from the cells) and found that the secreted polysaccharide i.e., EPS was predominant. The EPS showed good emulsifying activities against the petroleum hydrocarbons and its production was dependent on the carbon source supplied. The genome analysis also revealed genes involved in industrially important metabolites such as zeaxanthin pigment, ectoine and polyhydroxyalkanoate (PHA) biosynthesis. To confirm the genome data, we extracted these metabolites from the cultures and successfully identified them. The pigment extracted from the cells showed the distinct UV–Vis spectra having characteristic absorption peak of zeaxanthin (λmax 448 nm) with potent antioxidant activities. The ability of H. malpeensis strain YU-PRIM-29T to produce important biomolecules makes it an industrially important bacterium.

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Production of ectoine by Halomonas elongata BK-AG25 using osmotic shock technique

Cited by 8 publications

References 10 publications

Microalgae Bioactive Compounds to Topical Applications Products—A Review

Microalgae Bioactive Compounds to Topical Applications Products—A Review

High Salt Electrolyte Solutions Challenge the Electrochemical CO₂Reduction Reaction to Formate at Indium and Tin Cathodes

Genome analysis of a halophilic bacterium Halomonas malpeensis YU-PRIM-29T reveals its exopolysaccharide and pigment producing capabilities

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Production of ectoine by Halomonas elongata BK-AG25 using osmotic shock technique

Cited by 8 publications

References 10 publications

Microalgae Bioactive Compounds to Topical Applications Products—A Review

Microalgae Bioactive Compounds to Topical Applications Products—A Review

High Salt Electrolyte Solutions Challenge the Electrochemical CO2Reduction Reaction to Formate at Indium and Tin Cathodes

Genome analysis of a halophilic bacterium Halomonas malpeensis YU-PRIM-29T reveals its exopolysaccharide and pigment producing capabilities

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Product

Resources

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High Salt Electrolyte Solutions Challenge the Electrochemical CO₂Reduction Reaction to Formate at Indium and Tin Cathodes