Production of endo-1,4-?-glucanase and xylanase by Trichoderma reesei immobilized on polyurethane foam

Abstract

Endo-I,4-fI-glucanase and xylanase were produced by Trichodenna reesei immobilized on polyurethane foam using lactose as the main carbon source. The most porous carrier was found to be the best of those tested. The nitrogen source and KHzPO, concentration of the production medium had a marked effect on culture pH during the course of fermentation and, consequent&, on xylanase activity. An increase in lactose concentration from 7 to 27 gll resulted in an increase in endoglucanase activity (max. 730 VJml). xylanase activity (max. 3350 U/ml) and filter paper activity (max. 3.0 FPUIml).

Cost-effective production of cellulose hydrolysing enzymes from Trichoderma sp. RCK65 under SSF and its evaluation in saccharification of cellulosic substrates

“…Gomes et al [25] also reported presence of organic nitrogen in the medium lead to increase in production of hydrolysing enzymes by T. aurantiacus. Moreover, it has also been reported that nitrogen can significantly affect the pH of the medium during the course of fermentation as reported by Haapala et al [26], thereby influences the microbial metabolism. Similar findings were reported by Deswal et al [3] and Jain et al [12].…”

Optimization of physicochemical parameters for phenol biodegradation by Candida tropicalis PHB5 using Taguchi Methodology

“…This reveals that ammonium nitrate plays an important role in growth of C. tropicalis and biodegradation of phenol. However, the decrease in phenol degradation at the concentration beyond level 2 can be attributed to the verity that higher concentration of nitrogen source significantly affects the pH of the medium during growth and also influences the microbial metabolism [39].…”

Xylanases of Microorganisms

“…Òîìó ó äåÿêèõ гðèá³â, ÿê³ ðîñòóòü íà êñèëàí³, íå çàáðóäíåíîìó öåëþëîçîþ, ïðè íèçüêèõ ñï³ââ³äíîøåííÿõ àçîò/âóгëåöü â ñåðåäîâèù³ ìîжóòü ïðîäóêóâàòèñÿ êñèëàíîë³òè÷í³ фåðìåíòè, ÿê³ â³ëüí³ â³ä öåëþëàç. Îäíàê, âñòàíîâëåíî, ùî öåëþëîçîë³òè÷í³ ñóáñòðàòè º íåîáõ³äíèìè äëÿ ìàêñèìàëüíî¿ ïðîäóêö³¿ êñèëàíàçè Thermomonospora curvata [49] [26,36].…”

“…MCIB 59 [16]. Нàéá³ëüø ïîâíî îõàðàêòåðèçîâàíî êñèëàíàçè Aspergillus fumigatus і Trichоderma reesei [3], ïðè÷îìó îñòàííÿ ïðîÿâëÿº ìàêñèìàëüíó ñåðåä гðèá³â àêòèâí³ñòü (3350 ÌΕìë -1 ) [26,36].…”

“…Òîìó ó äåÿêèõ гðèá³â, ÿê³ ðîñòóòü íà êñèëàí³, íå çàáðóäíåíîìó öåëþëîçîþ, ïðè íèçüêèõ ñï³ââ³äíîøåííÿõ àçîò/âóгëåöü â ñåðåäîâèù³ ìîжóòü ïðîäóêóâàòèñÿ êñèëàíîë³òè÷í³ фåðìåíòè, ÿê³ â³ëüí³ â³ä öåëþëàç. Îäíàê, âñòàíîâëåíî, ùî öåëþëîçîë³òè÷í³ ñóáñòðàòè º íåîáõ³äíèìè äëÿ ìàêñèìàëüíî¿ ïðîäóêö³¿ êñèëàíàçè Thermomonospora curvata [49] [26,36].Ñõåìà î÷èñòêè åíäî (1 4) β êñèëàíàçè ç êóëüòóðàëüíî¿ ð³äèíè 3 õ äîáîâî¿ êóëüòóðè Geotrichum candidum 3Ñ áóëà ðîçðîáëåíà äîñë³äíèêàìè [2]. Âíàñë³äîê î÷èñòêè â 23 ðàçè ï³äâèùåíà ïèòîìà àêòèâí³ñòü åíäîêñèëàíàçè, ÿêà ñòàíîâèëà 32,6 е/ìг á³ëêà, âèõ³ä àêòèâíîñò³ -14,4 %.…”