Production of endothelial cell-enclosing alginate-based hydrogel fibers with a cell adhesive surface through simultaneous cross-linking by horseradish peroxidase-catalyzed reaction in a hydrodynamic spinning process

Liu, Yang; Sakai, Shinji; Taya, Masahito

doi:10.1016/j.jbiosc.2012.04.018

Cited by 36 publications

(15 citation statements)

References 24 publications

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“…By exploiting silk properties and enzymatic cross-linking methods, hydrogel fibers were fabricated with superior strength, extensibility, and long-term stability compared to previously established hydrogel microfibers. [6,7,28,29,31,[33][34][35][37][38][39][40][41][42][43][44][45] Fabrication and post-processing methods affected the overall water content in the fibers, ultimately driving changes in mechanical properties (Figures 1-3, Table 1). Post-processing for 608C and RT fabrication groups was necessary to drive water into the network ( Figure 3, Table 1).…”

Section: Discussionmentioning

confidence: 99%

Fabrication of elastomeric silk fibers

et al. 2017

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Methods to generate fibers from hydrogels, with control over mechanical properties, fiber diameter and crystallinity, while retaining cytocompatibility and degradability, would expand options for biomaterials. Here we exploited features of silk fibroin protein for the formation of tunable silk hydrogel fibers. The biological, chemical, and morphological features inherent to silk were combined with elastomeric properties gained through enzymatic crosslinking of the protein. Post-processing via methanol and autoclaving provided tunable control of fiber features. Mechanical, optical, and chemical analyses demonstrated control of fiber properties by exploiting the physical cross-links, and generating double network hydrogels consisting of chemical and physical cross-links. Structure and chemical analysis revealed crystallinity from 30–50%, modulus from 0.5MPa to 4MPa, and ultimate strength 1 to 5 MPa depending on the processing method. Fabrication and post-processing combined provided fibers with extensibility from 100 to 400% ultimate strain. Fibers strained to 100% exhibited 4th order birefringence, revealing macroscopic orientation driven by chain mobility. The physical cross-links were influenced in part by the drying rate of fabricated materials, where bound water, packing density, and micro-structural homogeneity influenced cross-linking efficiency. The ability to generate robust and versatile hydrogel microfibers is desirable for bottom-up assembly of biological tissues and for broader biomaterial applications.

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Section: Discussionmentioning

confidence: 99%

Fabrication of elastomeric silk fibers

et al. 2017

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“…Considering the practical applications of tissue fabrication in transplantation, the tubular cavity templated by the hydrogel fiber of 200 μm in diameter is very small. The diameter of hydrogel fibers can be easily increased during the preparation process as reported previously [ 15 , 16 , 22 ]. Because of the feasibility demonstrated in the current study, our next objective will be fabrication of larger tissue constructs by increasing the number of hydrogel fibers and/or the diameter of the fiber to facilitate medium flow throughout the tissues.…”

Section: Resultsmentioning

confidence: 96%

“…Hydrogel fibers were prepared based on a reported method using a double co-axial cylinder system designed in our laboratory [ 15 , 16 , 22 ]. Briefly, PBS containing 4% (w/v) Alg-Ph and 100 units/mL HRP was extruded from a 25-G stainless steel needle at 0.15 mL/min into a co-flowing immiscible fluid of PBS containing 0.1% (w/v) gelatin-Ph and 0.3 mM H 2 O 2 extruded from an 18-G stainless steel needle at 4 mL/min.…”

Section: Methodsmentioning

confidence: 99%

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Engineering tissues with a perfusable vessel-like network using endothelialized alginate hydrogel fiber and spheroid-enclosing microcapsules

Liu

Sakai

Taya

2016

Heliyon

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Development of the technique for constructing an internal perfusable vascular network is a challenging issue in fabrication of dense three-dimensional tissues in vitro. Here, we report a method for realizing it. We assembled small tissue (about 200 μm in diameter)-enclosing hydrogel microcapsules and a single hydrogel fiber, both covered with human vascular endothelial cells in a collagen gel. The microcapsules and fiber were made from alginate and gelatin derivatives, and had cell adhesive surfaces. The endothelial cells on the hydrogel constructs sprouted and spontaneously formed a network connecting the hydrogel constructs with each other in the collagen gel. Perfusable vascular network-like structure formation after degrading the alginate-based hydrogel constructs by alginate lyase was confirmed by introducing solution containing tracer particles of about 3 μm in diameter into the lumen templated by the alginate hydrogel fiber. The introduced solution flowed into the spontaneously formed capillary branches and passed around the individual spherical tissues.

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“…Fibers formed using microfluidic devices have been used to encapsulate various types of cells, including islet cells for immunoprotection reported by Jun et al (2013). Liu et al (2012b) reported a process to generate homogeneous fibers of alginate, modified with the phenolic hydroxyl group, which were obtained by enzymatically crosslinking the phenolic moieties. Fig.…”

Section: Continuous Production Of Homogeneous Bersmentioning

confidence: 99%

Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

Yamada

Seki

2018

Journal of Chemical Engineering of Japan

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With the recent developments in micro uidic instruments and devices, various types of micrometer-sized materials have been produced by employing multiphase ow patterns formed in the microchannel. In particular, microparticles and micro bers, which are compatible with biomolecule incorporation or living cell encapsulations, have been gaining signi cant attention as new tools for biochemical analysis, cellular physiological studies, tissue engineering, cell transplantation, and controlled drug delivery. Herein, we introduce recent developments in micro uidic systems to produce alginate-based hydrogel microparticles and micro bers. By utilizing droplet dispersions either in equilibrium or nonequilibrium states, or by employing parallel laminar ows, microengineered functional materials that are di cult to generate using conventional devices and operations can be obtained. New and interesting multiphase phenomena are reviewed, together with the pros and cons of these systems and their applications. Furthermore, the fundamentals of multiphase micro uidics and the materials used to prepare particles and bers are brie y introduced.

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Production of endothelial cell-enclosing alginate-based hydrogel fibers with a cell adhesive surface through simultaneous cross-linking by horseradish peroxidase-catalyzed reaction in a hydrodynamic spinning process

Cited by 36 publications

References 24 publications

Fabrication of elastomeric silk fibers

Fabrication of elastomeric silk fibers

Engineering tissues with a perfusable vessel-like network using endothelialized alginate hydrogel fiber and spheroid-enclosing microcapsules

Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

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