Production of Enniatins by Fusarium sambucinum: Selection of High-yield Conditions from Liquid Surface Cultures

Abstract

SUMMARYEnniatins were determined in the mycelium of Fusarium sambucinum grown in liquid surface culture on semidefined or undefined media. Light was required for significant enniatin production, which was also favoured by moderate growth temperature. Production was supported by various carbon and nitrogen sources.Growth on a medium containing (w/v) 5 % lactose and 0.8 % tryptone, at 20

Regulation by light in Fusarium

“…Enniatin production was enhanced three fold upon growth of F. sambucinum under continuous illumination and four fold under 8 h or 12 h photocycles, compared to dark-grown cultures (Audhya and Russell, 1974). As found for gibberellin biosynthesis, these variations were minor compared to those produced by changes in media composition, with the nitrogen source as the most relevant regulatory signal.…”

Nematicidal Substances from Fungi

“…Other two cyclodepsipeptides, enniatin A (44) and enniatin B (45), were isolated from the culture broth of Fusarium spp. [44,45], and both were weakly active against M. incognita [18]. Enniatin B was also reported to possess a nematicidal activity against A. aceti [26].…”

Regulation of secondary metabolism in fungi

“…Temperatures lower than that required for optimum growth are required for maximum production of enniatins (66) and aflatoxins (25). In the production of aflatoxins, temperature shifts upward during the fermentation appear to markedly increase production on a solid substrate (rice) (69).…”