Production of enterotoxins and toxic shock syndrome toxin by bovine mammary isolates of Staphylococcus aureus

Kenny, Kevin; Reiser, Raoul F.; Bastida, Felix; Norcross, N. L.

doi:10.1128/jcm.31.3.706-707.1993

Cited by 86 publications

(47 citation statements)

References 21 publications

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“…The value of 65·04% of bovine isolates secreting TSST-1 and/or one or more enterotoxins is much greater than those of previous studies, where up to 52·0% of strains isolated from bovine mastitis produced enterotoxins (Lopes et al 1990;Kenney et al 1993;Matsunaga et al 1993;Aarestrup et al 1995;Ichikawa et al 1996;Bogni et al 1997). Moreover, these authors reported that enterotoxin C is the predominant type produced by bovine isolates of S. aureus, in contrast with human isolates, in which A is the main type.…”

Section: Discussionmentioning

confidence: 67%

“…According to Kenney et al (1993) and Aarestrup et al (1995) these results could be caused by differences between the strains and sources of S. aureus. In addition, Kenney et al (1993) suggested that differences might be a consequence of the concentration of proteins prior to immunodiffusion. In fact, some authors, such as Lopes et al (1990) (1997), have reported related a low level (4·5%) of S. aureus strains producing enterotoxins when detected by the OSP method.…”

Section: Discussionmentioning

confidence: 99%

“…Toxic shock syndrome toxin 1 (TSST-1) is the causative agent in toxic shock syndrome in humans, and enterotoxins (SEs) may thus play a role in staphylococcal disease states, in addition to their ability to cause food poisoning. TSST-1 and staphylococcal enterotoxin a (SEA), SEC and SED have been detected among isolates from bovine mastitis and young goats around the world where staphylococcal strains isolated produced one or several toxins (Kenney et al 1993;Matsunaga et al 1993;Bezek & Hull 1995;Bogni et al 1997;Takeuchi et al 1998). Although the S. aureus contributes significantly to disease in animals, there is little information concerning the frequency of S. aureus strains producing those toxins and the type of toxins in isolates from cows in Brazil (Lopes et al 1990).…”

Section: Introductionmentioning

confidence: 99%

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Production of enterotoxins and toxic shock syndrome toxin by Staphylococcus aureus isolated from bovine mastitis in Brazil

Cardoso

Silva

Sena

et al. 1999

Lett Appl Microbiol

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The production of toxic shock syndrome toxin 1 (TSST‐1) and enterotoxins (SE) A, B, C and D by bovine mastitis isolates of Staphylococcus aureus was evaluated by immunodiffusion using the Optimum‐Sensitivity Plate method. S. aureus strains were isolated from bovine mastitis in 23 dairy herds in the state of Minas Gerais, Brazil, during 1994–9. Of 127 isolates, 83 (65·04%) produced one or several toxins, and among them production of SE was found in 54 (43·0%) isolates, of which 1138 (29·09%) secreted enterotoxin identified as type D. TSST‐1 was found in 5829 (45·723·0%) isolates.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production of enterotoxins and toxic shock syndrome toxin by Staphylococcus aureus isolated from bovine mastitis in Brazil

Cardoso

Silva

Sena

et al. 1999

Lett Appl Microbiol

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“…A study by Kenny et al (1993) of isolates recovered from cows in the USA showed that ca 20% of isolates produced TSST-1, generally in association with SEC and SED. In the present study, a similar proportion of isolates produced SEC and TSST-1 (16%) in detectable quantities.…”

Section: Discussionmentioning

confidence: 99%

Molecular population and virulence factor analysis of Staphylococcus aureus from bovine intramammary infection

Fitzgerald¹,

Hartigan

Meaney

et al. 2000

J Appl Microbiol

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Ireland (n 102) and the USA (n 42) were characterized by RAPD-PCR and analysed for the production of a number of putative virulence factors. Of these strains 63 representative isolates were screened for the corresponding virulence factor genes by PCR or Southern hybridization or both. The isolates were divided into 12 distinct clonal types on the basis of their RAPD ®ngerprint pro®les. Of the isolates, 107 (74Á3%) tested positive for clumping factor in a slide agglutination test, all 24 RAPD type 7 isolates being negative for clumping factor. PCR analysis of region R, a repeat region of the clfA gene, revealed eight region-R sizes. There was a strong association between RAPD type and the clfA region-R genotype among Irish isolates. Of the RAPD type 7 isolates, 21 (87Á5%) coproduced toxic shock syndrome toxin-1 (TSST-1) and staphylococcal enterotoxin C (SEC). Over 90% of isolates demonstrated haemolytic activity on sheep or rabbit red blood cells and all isolates harboured the gamma-haemolysin (hlg) locus. Of the Irish isolates, all those of RAPD type 7 were sensitive to penicillin G, whereas 86% of RAPD types 4 and 5 strains were resistant. Furthermore, RAPD types 5 and 7 were more likely to be associated with clinical mastitis whereas RAPD type 4 isolates were more often associated with a latent infection. The current study identi®es some of the putative virulence factors produced by the predominant clonal types of bovine Staph. aureus that may be considered as components of a vaccine.

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“…The strains were isolated from severe cases of bovine mastitis in a closed Frisian herd. Kenny et al 1993 found 28AE6% of enterotoxigenic Staph. aureus bovine isolates from mammary gland.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and clonal relatedness of sec/tst-gene positive Staphylococcus aureus isolates of quartermilk samples of cows suffering from mastitis

Zschöck¹,

Risse²,

Sommerhäuser³

2004

Lett Appl Microbiol

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Aims: To investigate the prevalence of sec/tst-gene positive Staphylococcus aureus in bovine mastitis and to get information about the clonal relatedness of these clinical isolates. Methods and Results: A total of 533 Staph. aureus strains isolated from bovine mastitic quartermilk samples at 493 randomized dairy farms in Hessia, Germany, from January 1997 until June 1998 were examined for enterotoxin C (sec) gene and toxic shock syndrome toxin (tst) gene by multiplex polymerase chain reaction. Fiftythree (9AE3%) of the strains were sec/tst-gene positive. Phenotypic TSST-1 production was found in all positive strains by reversed passive latex agglutination test. With DNA macrorestriction analysis, sec/tst-gene positive strains were divided into five different macrorestriction types. Type I (10 isolates) and III (40 isolates) were found to be the predominant types in terms of frequency of isolation in the investigated area. These DNA macrorestriction types differed in only two bands in the 500 and 270 bp region. Conclusions: Closely related Staph. aureus strains seem to be responsible for an unusual large proportion of bovine mastitis cases in geographically widely distinct locations. Significance and Impact of the Study: This is one of the first reports about the relatedness of sec/tst-gene positive Staph. aureus clinical isolates from bovine mastitis.

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Production of enterotoxins and toxic shock syndrome toxin by bovine mammary isolates of Staphylococcus aureus

Cited by 86 publications

References 21 publications

Production of enterotoxins and toxic shock syndrome toxin by Staphylococcus aureus isolated from bovine mastitis in Brazil

Production of enterotoxins and toxic shock syndrome toxin by Staphylococcus aureus isolated from bovine mastitis in Brazil

Molecular population and virulence factor analysis of Staphylococcus aureus from bovine intramammary infection

Occurrence and clonal relatedness of sec/tst-gene positive Staphylococcus aureus isolates of quartermilk samples of cows suffering from mastitis

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