Production of Erythritol by Newly Isolated Osmophilic Trichosporon sp.

Abstract

One strain of osmophilic Trichosporon sp. which produces highest amounts of erythritol, was isolated from honeycomb. This strain produced highest amounts of erythritol with total glucose utilization in fermentation medium at 35°C and a temperature of 30°C results in an insufficient glucose utilization and lower production of erythritol, but higher growth of cell mass as compared with fermentation temperature of 35°C. An efficient fermentation medium for production of erythritol by the strain was mixture of 30% glucose and 4% corn steep liquor, and using this culture medium, the strain produced 141 g/l of erythritol at 35°C after 3 days of fermentation in 5001‐fermentor. It was also found that yield of microbial cells (Yx/S) to yield of erythritol (Ye/S) was 1:1 during phase of yeast growth and the proportion was changes to 1:10 during stationary phase.

High-level production of erythritol by mutants of osmophilic Moniliella sp.

“…2), which are superior to erythritol production of 142 g/L from Trichosporon sp. in a 500-L fermentor [9]. In addition, as shown in Fig.…”

“…[6][7][8][9]. It has also been reported that Leuconostoc oenos produces erythritol, but only under anaerobic conditions [10].…”

Erythritol production by yeasts: a snapshot of current knowledge

“…Several yeast genera have been used for erythritol production, namely Aureobasidium (Ishizuka et al, ), Trichosporonoides (Aoki, Pastore, & Park, ), Moniliella (Lin, Wen, Liau, & Chu, ), Trigonopsis (Kim, Lee, Kim, & Oh, ), Trichosporon (Park, Yook, & Park, ), Torula (Lee, Ha, Kim, & Oh, ), Candida (Ryu, Park, Park, Kim, & Seo, ), Pseudozyma (Jeya et al, ) and Yarrowia (Rymowicz, Rywińska, & Gładkowski, ) (Table ). In order to improve erythritol productivity or yield, some of these strains were modified genetically.…”

“…Aureobasidium (Ishizuka et al, 1989), Trichosporonoides (Aoki, Pastore, & Park, 1993), Moniliella (Lin, Wen, Liau, & Chu, 2001), Trigonopsis (Kim, Lee, Kim, & Oh, 1997), Trichosporon (Park, Yook, & Park, 1998b), Torula (Lee, Ha, Kim, & Oh, 2000), Candida (Ryu, Park, Park, Kim, & Seo, 2000), Pseudozyma (Jeya et al, 2009)…”

Comparison of propolis from Apis mellifera and Tetragonisca angustula

“…As another example, butanetriol was characterized only in the methanol extract of propolis from T. angustula, and the cyclic polyhydroxy alcohol inositol was found only in the methanol extract of propolis from A. mellifera. Park et al (1998Park et al ( , 2001) isolated microorganisms from pollen of Mangifera sp. (Anacardiaceae) and from Brazilian honeys, which were able to convert sucrose, glucose and fructose to erythritol.…”