“…S. cerevisiae, the principal yeast used for wine fermentation, preferentially uses many simple nitrogen sources such as free alpha amino nitrogen compounds, present in the form of primary amino acids and leaves only minor concentrations of other nitrogen compounds (Cooper, 1982;Bisson, 1991;Monteiro and Bisson, 1991;Henschke and Jiranek, 1993;Jiranek et al, 1995). Additionally, S. cerevisiae is unable to use nitrates and nitrites and in certain conditions the amino acids proline and hydroxyproline which are not metabolised under winemaking conditions (Duteurtre et al, 1971;Ingledew et al, 1987;Suresh et al, 1999). Thus, certain amino acids are available at the end of alcoholic fermentation, and others even increase by release during autolysis, namely: proline, leucine, tryptophan and gamma aminobutyric acid (Lehtonen, 1996;Valero et al, 2003).…”