Production of Ethanol from Starch by Respiration-Deficient Recombinant
            <i>Saccharomyces cerevisiae</i>

Öner, Ebru Toksoy; Oliver, Stephen G.; Kırdar, Betül

doi:10.1128/aem.71.10.6443-6445.2005

Cited by 45 publications

(10 citation statements)

References 20 publications

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“…A spontaneous petite mutant was also observed to ferment wort more slowly and accumulate less biomass, at low cell densities, than that of its respiratory‐competent wild‐type99, 200, 265. Reduced attenuation rates73 but higher ethanol yields132, 265 appear to occur. Spontaneous mutants also appear to modify beer flavour191 by overproducing higher alcohols, such as isobutanol and isoamyl alcohol, and underproducing esters99, 191, 199.…”

Section: Brewing Yeast Genome Instabilitymentioning

confidence: 98%

“…Petite mutation affects sugar utilization in brewing strains73, 139. A spontaneous petite mutant was also observed to ferment wort more slowly and accumulate less biomass, at low cell densities, than that of its respiratory‐competent wild‐type99, 200, 265. Reduced attenuation rates73 but higher ethanol yields132, 265 appear to occur.…”

Section: Brewing Yeast Genome Instabilitymentioning

confidence: 99%

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Brewing yeast genomes and genome‐wide expression and proteome profiling during fermentation

Smart

2007

Yeast

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The genome structure, ancestry and instability of the brewing yeast strains have received considerable attention. The hybrid nature of brewing lager yeast strains provides adaptive potential but yields genome instability which can adversely affect fermentation performance. The requirement to differentiate between production strains and assess master cultures for genomic instability has led to significant adoption of specialized molecular tool kits by the industry. Furthermore, the development of genome-wide transcriptional and protein expression technologies has generated significant interest from brewers. The opportunity presented to explore, and the concurrent requirement to understand both, the constraints and potential of their strains to generate existing and new products during fermentation is discussed.

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Section: Brewing Yeast Genome Instabilitymentioning

confidence: 98%

Section: Brewing Yeast Genome Instabilitymentioning

confidence: 99%

Brewing yeast genomes and genome‐wide expression and proteome profiling during fermentation

Smart

2007

Yeast

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“…Regardless of the species, parental microorganisms remain insufficient in conventional ethanol production due to the lack of availability of sugar rich input and low ethanol yields. Over 150 amylolytic yeast strains have been reported to be impractical in industrial use because of limited characteristics [13]. Although Clostridium spp.…”

Section: Introductionmentioning

confidence: 99%

Genetic Modifications of Saccharomyces cerevisiae for Ethanol Production from Starch Fermentation: A Review

Aydemir¹

2014

J Bioproces Biotech

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There is a huge demand for developing new technologies for alternative energy sources due to the elevated costs of petroleum and its by-products, depletion of nonrenewable fuel sources, and to eliminate the disadvantages of geopolitical location and environmental pollution caused by high levels of carbon dioxide release. Science is striving to meet this demand and as molecular biology techniques have progressed, genetic engineering tools have been presented as promising future solutions in the form of optimizing the fermentation process to increase the ethanol yield from different carbon sources such as starch. As Saccharomyces cerevisiae is not naturally able to ferment starch, it can be genetically manipulated and modulated to improve the fuel production from starchy materials and the amount of cost that is required to produce ethanol would be decreased with these manipulations. General modifications in S. cerevisiae include specific gene expressions to gain new properties or improve existing pathways. This review aims to elicit the current status of ethanol production thorough alternative techniques from starch using current genetic engineering applications and to give further directions for high-throughput fermentations using genetically modified S. cerevisiae strains.

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“…Hutter and Oliver (18) suggested that 100% respiration-deficient nuclear petites may be used in the commercial production of ethanol under circumstances in which the oxygen supply cannot be tightly controlled. Indeed, the use of respiration-deficient S. cerevisiae strains as hosts for the construction of recombinant strains increased the ethanol yield from starch (38).…”

mentioning

confidence: 99%

Integration of Metabolic Modeling and Phenotypic Data in Evaluation and Improvement of Ethanol Production Using Respiration-Deficient Mutants of Saccharomyces cerevisiae

Dikicioglu

Pir

Önsan

et al. 2008

Appl Environ Microbiol

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Flux balance analysis and phenotypic data were used to provide clues to the relationships between the activities of gene products and the phenotypes resulting from the deletion of genes involved in respiratory function in Saccharomyces cerevisiae. The effect of partial or complete respiratory deficiency on the ethanol production and growth characteristics of hap4⌬/hap4⌬, mig1⌬/mig1⌬, qdr3⌬/qdr3⌬, pdr3⌬/pdr3⌬, qcr7⌬/ qcr7⌬, cyt1⌬/cyt1⌬, and rip1⌬/rip1⌬ mutants grown in microaerated chemostats was investigated. The study provided additional evidence for the importance of the selection of a physiologically relevant objective function, and it may improve quantitative predictions of exchange fluxes, as well as qualitative estimations of changes in intracellular fluxes. Ethanol production was successfully predicted by flux balance analysis in the case of the qdr3⌬/qdr3⌬ mutant, with maximization of ethanol production as the objective function, suggesting an additional role for Qdr3p in respiration. The absence of similar changes in estimated intracellular fluxes in the qcr7⌬/qcr7⌬ mutant compared to the rip1⌬/rip1⌬ and cyt1⌬/cyt1⌬ mutants indicated that the effect of the deletion of this subunit of complex III was somehow compensated for. Analysis of predicted flux distributions indicated self-organization of intracellular fluxes to avoid NAD ؉ /NADH imbalance in rip1⌬/rip1⌬ and cyt1⌬/ cyt1⌬ mutants, but not the qcr7⌬/qcr7⌬ mutant. The flux through the glycerol efflux channel, Fps1p, was estimated to be zero in all strains under the investigated conditions. This indicates that previous strategies for improving ethanol production, such as the overexpression of the glutamate synthase gene GLT1 in a GDH1 deletion background or deletion of the glycerol efflux channel gene FPS1 and overexpression of GLT1, are unnecessary in a respiration-deficient background.

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Production of Ethanol from Starch by Respiration-Deficient Recombinant Saccharomyces cerevisiae

Cited by 45 publications

References 20 publications

Brewing yeast genomes and genome‐wide expression and proteome profiling during fermentation

Brewing yeast genomes and genome‐wide expression and proteome profiling during fermentation

Genetic Modifications of Saccharomyces cerevisiae for Ethanol Production from Starch Fermentation: A Review

Integration of Metabolic Modeling and Phenotypic Data in Evaluation and Improvement of Ethanol Production Using Respiration-Deficient Mutants of Saccharomyces cerevisiae

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