Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade

Li, Kai; Sun, Weikang; Meng, Wensi; Yan, Jinxin; Zhang, Yipeng; Guo, Shiting; Lü, Chuanjuan; Ma, Cuiqing; Gao, Chao

doi:10.3390/catal11020214

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…1040−1042 A recent study produced bio-EG from glycerol via an enzymatic cascade through glycolaldehyde, and yielded 0.52 g EG/g glycerol, the highest reported yield from any route to date. 1043 The current price of EG is low due to established large scale industrial production, and titers and yields of EG should exceed 100 g L −1 and 0.5 g g −1 feedstock before presenting bio-EG as a competitive alternative.…”

Section: Chemicalmentioning

confidence: 99%

“…The carbon utilization of these pathways is limited to 40% as the 5-carbon pentose is converted into a 2-carbon glycolaldehyde and a 3-carbon intermediate, with only the former converted into EG . The combination of these pathways with a serine synthesis pathway allows utilization of the 3-carbon intermediates to produce additional glycolaldehyde, doubling the maximum total conversion of pentose carbon to 80%, but this requires significant further work. − A recent study produced bio-EG from glycerol via an enzymatic cascade through glycolaldehyde, and yielded 0.52 g EG/g glycerol, the highest reported yield from any route to date . The current price of EG is low due to established large scale industrial production, and titers and yields of EG should exceed 100 g L –1 and 0.5 g g –1 feedstock before presenting bio-EG as a competitive alternative.…”

Section: Bisfunctional Monomers Used In the Synthesis Of Commodity Th...mentioning

confidence: 99%

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Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers

et al. 2022

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Access to a wide range of plastic materials has been rationalized by the increased demand from growing populations and the development of high-throughput production systems. Plastic materials at low costs with reliable properties have been utilized in many everyday products. Multibillion-dollar companies are established around these plastic materials, and each polymer takes years to optimize, secure intellectual property, comply with the regulatory bodies such as the Registration, Evaluation, Authorisation and Restriction of Chemicals and the Environmental Protection Agency and develop consumer confidence. Therefore, developing a fully sustainable new plastic material with even a slightly different chemical structure is a costly and long process. Hence, the production of the common plastic materials with exactly the same chemical structures that does not require any new registration processes better reflects the reality of how to address the critical future of sustainable plastics. In this review, we have highlighted the very recent examples on the synthesis of common monomers using chemicals from sustainable feedstocks that can be used as a like-for-like substitute to prepare conventional petrochemical-free thermoplastics.

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Section: Chemicalmentioning

confidence: 99%

Section: Bisfunctional Monomers Used In the Synthesis Of Commodity Th...mentioning

confidence: 99%

Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers

et al. 2022

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“…As monomers of poly(ethylene furanoate) (PEF), ethylene glycol and 2,5-furandicarboxylic acid (2,5-FDCA) could also be obtained through biocatalysis. Ethylene glycol could be synthesized using glycerol as the raw material through the artificial enzymatic cascade containing alditol oxidase, catalase, glyoxylate/hydroxypyruvate reductase, pyruvate decarboxylase and lactaldehyde:propanediol oxidoreductase ( Li et al, 2021 ). FDCA could be obtained using 5-hydroxymethylfurfural (HMF) as the raw material catalyzed by 5-hydroxymethylfurfural oxidase and Novozym 435 ( Wu et al, 2020 ).…”

Section: Materials For Producing Bio-plasticsmentioning

confidence: 99%

Enzyme catalyzes ester bond synthesis and hydrolysis: The key step for sustainable usage of plastics

et al. 2023

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Petro-plastic wastes cause serious environmental contamination that require effective solutions. Developing alternatives to petro-plastics and exploring feasible degrading methods are two solving routes. Bio-plastics like polyhydroxyalkanoates (PHAs), polylactic acid (PLA), polycaprolactone (PCL), poly (butylene succinate) (PBS), poly (ethylene furanoate) s (PEFs) and poly (ethylene succinate) (PES) have emerged as promising alternatives. Meanwhile, biodegradation plays important roles in recycling plastics (e.g., bio-plastics PHAs, PLA, PCL, PBS, PEFs and PES) and petro-plastics poly (ethylene terephthalate) (PET) and plasticizers in plastics (e.g., phthalate esters, PAEs). All these bio- and petro-materials show structure similarity by connecting monomers through ester bond. Thus, this review focused on bio-plastics and summarized the sequences and structures of the microbial enzymes catalyzing ester-bond synthesis. Most of these synthetic enzymes belonged to α/β-hydrolases with conserved serine catalytic active site and catalyzed the polymerization of monomers by forming ester bond. For enzymatic plastic degradation, enzymes about PHAs, PBS, PCL, PEFs, PES and PET were discussed, and most of the enzymes also belonged to the α/β hydrolases with a catalytic active residue serine, and nucleophilically attacked the ester bond of substrate to generate the cleavage of plastic backbone. Enzymes hydrolysis of the representative plasticizer PAEs were divided into three types (I, II, and III). Type I enzymes hydrolyzed only one ester-bond of PAEs, type II enzymes catalyzed the ester-bond of mono-ester phthalates, and type III enzymes hydrolyzed di-ester bonds of PAEs. Divergences of catalytic mechanisms among these enzymes were still unclear. This review provided references for producing bio-plastics, and degrading or recycling of bio- and petro-plastics from an enzymatic point of view.

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“…After 10 hours, 7.64 mM EG was produced from 10 mM glycerol using this in vitro synthesis system. 123…”

Section: Biosynthesis Of Tpa Fdca and Egmentioning

confidence: 99%