Production of extracellular H2O2 and L-lysine-α-oxidase during bulk growth of the fungus Trichoderma cf. aureoviride Rifai VKM F-4268D under salt stress

Arinbasarova, A. Yu.; Makrushin, K. V.; Меденцев, А. Г.; Лукашева, Е. В.; Березов, Т. Т.

doi:10.1134/s0026261712050025

Cited by 2 publications

(3 citation statements)

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“…In Figure 1B, one can see the dynamics of the accumulation of LysO and proteolytic activity, which hydrolyze the protein components of wheat bran and ensure the presence of amino acids in the growth medium, including lysine. Other than lysine, more than 10 amino acids, including lysine, were detected in the medium [7].…”

Section: Resultsmentioning

confidence: 99%

“…It is also known that fungi are capable of synthesizing exogenous L-amino acid oxidases, including L-lysine-α-oxidase (LysO) [4][5][6][7]. LysO attracts the interest of researchers due to perspectives for its applications in biotechnology and medicine [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…It is known that Trichoderma fungi grow using various media containing simple carbohydrates (glucose, sucrose, starch, etc. ), as well as complex substrates, including wheat bran [6,7,15,16] or the cell wall of phytopatogenic fungi [3,5,17]. To grow on these complex substrates, fungi need to synthesize a complex of lytic enzymes (xylanases, glucanases, proteases, etc.)…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Extracellular L-lysine-α-oxidase along with Degrading Enzymes by Trichoderma cf. aureoviride Rifai VKM F-4268D: Role in Biocontrol and Systemic Plant Resistance

Arinbasarova,

Botin,

Medentsev

et al. 2024

JoF

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When cultivating on wheat bran or deactivated fungal mycelium as a model of “natural growth”, the ability of Trichoderma to synthesize extracellular L-lysine-α-oxidase (LysO) simultaneously with cell-wall-degrading enzymes (proteases, xylanase, glucanases, chitinases, etc.), responsible for mycoparasitism, was shown. LysO, in turn, causes the formation of H2O2 and pipecolic acid. These compounds are known to be signaling molecules and play an important role in the induction and development of systemic acquired resistance in plants. Antagonistic effects of LysO have been demonstrated against phytopathogenic fungi and Gram-positive or Gram-negative bacteria with dose-dependent cell death. The antimicrobial effect of LysO decreased in the presence of catalase. The generating intracellular ROS in the presence of LysO was also shown in both bacteria and fungi, which led to a decrease in viable cells. These results suggest that the antimicrobial activity of LysO is due to two factors: the formation of exogenous hydrogen peroxide as a product of the enzymatic oxidative deamination of L-lysine and the direct interaction of LysO with the cell wall of the micro-organisms. Thus, LysO on its own enhances the potential of the producer in the environment; namely, the enzyme complements the strategy of the fungus in biocontrol and indirectly participates in inducing SAR and regulating the relationship between pathogens and plants.

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Section: Resultsmentioning

confidence: 99%