Production of extracellular polymeric substances in granular sludge under selection for<i>Accumulibacter</i>and<i>Competibacter</i>

Guimarães, Lorena; Gubser, Nina; Lin, Yuemei; Zlopaša, Jure; Felz, Simon; Tomás-Martínez, Sergio; Pronk, Mario; Neu, Thomas R.; Dueholm, Morten Simonsen; Albertsen, Mads; Costa, Rejane Helena Ribeiro da; Nielsen, Per Halkjær; Loosdrecht, M.C.M. van; Weissbrodt, David G.

doi:10.1101/2023.03.24.534144

Cited by 3 publications

(1 citation statement)

References 110 publications

(194 reference statements)

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“…(Seviour et al, 2012) and others associated with the production of a resistant matrix of structural EPS like Ca. Accumulibacter (Guimarães et al, 2023) substantially increased in abundance. Members of the Rhogostoma protistan group decreased in relative abundance in favor of other micro-eukaryotes within the Cryptomonadales , Rotifera and Sessilida groups.…”

Section: Discussionmentioning

confidence: 99%

Community successional patterns and inter-kingdom interactions during granular biofilm development

de Celis,

Modin,

Arregui

et al. 2024

Preprint

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Aerobic granular sludge is a compact and efficient biofilm process used for wastewater treatment which has received much attention and is currently being implemented worldwide. The microbial associations and their ecological implications occurring during granule development, especially those involving inter-kingdom interactions, are poorly understood. In this work, we monitored the prokaryote and eukaryote community composition and structure during the granulation of activated sludge for 343 days in a sequencing batch reactor (SBR), and investigated the influence of abiotic and biotic factors on the granule development. Sludge granulation was accomplished with low-wash out dynamics at long settling times, allowing for the microbial communities to adapt to the SBR environmental conditions. The sludge granulation and associated changes in microbial community structure could be divided into three stages: floccular, intermediate, and granular. The eukaryotic and prokaryotic communities showed parallel successional dynamics, with three main sub-communities identified for each kingdom, dominating in each stage of sludge granulation. Although inter-kingdom interactions were shown to affect community succession during the whole experiment, during granule development random factors like the availability of settlement sites or drift acquired increasing importance. The prokaryotic community was more affected by deterministic factors, including reactor conditions, while the eukaryotic community was to a larger extent shaped by biotic interactions (including inter-kingdom interactions) and stochasticity.

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Section: Discussionmentioning

confidence: 99%

Community successional patterns and inter-kingdom interactions during granular biofilm development

de Celis,

Modin,

Arregui

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Community successional patterns and inter-kingdom interactions during granular biofilm development

de Celis,

Modin,

Arregui

et al. 2024

npj Biofilms Microbiomes

View full text Add to dashboard Cite

Aerobic granular sludge is a compact and efficient biofilm process used for wastewater treatment which has received much attention and is currently being implemented worldwide. The microbial associations and their ecological implications occurring during granule development, especially those involving inter-kingdom interactions, are poorly understood. In this work, we monitored the prokaryote and eukaryote community composition and structure during the granulation of activated sludge for 343 days in a sequencing batch reactor (SBR) and investigated the influence of abiotic and biotic factors on the granule development. Sludge granulation was accomplished with low-wash-out dynamics at long settling times, allowing for the microbial communities to adapt to the SBR environmental conditions. The sludge granulation and associated changes in microbial community structure could be divided into three stages: floccular, intermediate, and granular. The eukaryotic and prokaryotic communities showed parallel successional dynamics, with three main sub-communities identified for each kingdom, dominating in each stage of sludge granulation. Although inter-kingdom interactions were shown to affect community succession during the whole experiment, during granule development random factors like the availability of settlement sites or drift acquired increasing importance. The prokaryotic community was more affected by deterministic factors, including reactor conditions, while the eukaryotic community was to a larger extent shaped by biotic interactions (including inter-kingdom interactions) and stochasticity.

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Biopolymer Production in a Full-Scale Activated Sludge Wastewater Treatment Plant: Seasonal Changes and Promising Bacterial Producers

Cydzik-Kwiatkowska,

Ciesielski,

Florczyk

et al. 2024

Energies

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Wastewater treatment plants (WWTPs) offer the possibility of recovering valuable substances produced by microorganisms, such as extracellular polymeric substances (EPSs). This study aimed to investigate the generation and properties of biopolymers and microbial communities of activated sludge from a large, full-scale WWTP. EPS composition in the activated sludge changed mostly during the transition period from winter to spring. Higher temperatures favored higher protein (PN) concentrations and a higher PN/PSs (polysaccharides) ratio in tightly-bound EPS, stimulating bacterial aggregation. In the sludge, filamentous Microthrix sp. were abundant (~6%) but the settling properties of the sludge improved with increasing PN content in the bound EPS fraction. The content of alginate (ALE)-like polymers averaged 55–60 mg/g Mixed Liquor Suspended Solids, and ALE content in sludge and characteristics were stable during the year. The abundance of Nitrospira sp. and the marine group NS9 in activated sludge correlated with the ALE content in the biomass, pointing to the importance of biopolymer production for nitrogen-transforming microorganisms. The most common EPS-producer was, Candidatus Competibacter (3–4%). The abundance of the Roseiflexaceae family significantly increased in summer, as did the abundance of Trichoccus sp. and Flavobacterium sp. in winter. The study shows that seasonal temperature fluctuations do not significantly affect the production of polymers, especially alginate, which favors commercial ALE recovery. The non-uniform composition of ALE-like polymers shows the possibility of their use in areas that do not require a specific polymer composition, e.g., as environmentally friendly coating materials or sorbents. The study contributes to biopolymer recovery and valorization of activated sludge.

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Production of extracellular polymeric substances in granular sludge under selection forAccumulibacterandCompetibacter

Cited by 3 publications

References 110 publications

Community successional patterns and inter-kingdom interactions during granular biofilm development

Community successional patterns and inter-kingdom interactions during granular biofilm development

Community successional patterns and inter-kingdom interactions during granular biofilm development

Biopolymer Production in a Full-Scale Activated Sludge Wastewater Treatment Plant: Seasonal Changes and Promising Bacterial Producers

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