Production of extracellular protease in submerged fermentation by Bacillus licheniformis ATCC 12759

Akcan, Nurullah

doi:10.5897/ajb11.2183

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…A protease is an enzyme that carries out the hydrolysis of peptide bonds between the amino acids occurring in a polypeptide chain forming the protein and cause their splitting into free amino A acids [14] .Proteases have found applications in diverse fields such as detergent industry, leather processing, silk degumming, food and dairy, baking, pharmaceutical industries, silver recovery from x-ray films, waste management and others. This high demand of proteases has triggered the scientific community to isolate novel strains and optimize the process parameters for the production of proteases [15].…”

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confidence: 99%

Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

Hamid

Jehangir

Baba

et al. 2019

Preprint

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The temperate climatic regions face the problem of waste accumulation due to lower environmental temperatures. However, these regions harbor cold active microbes viz. psychrotrophic proteolytic bacteria that play an important role in the degradation of protenaceous materials of the waste stream. Hence in the present study psychrotrophic proteolytic bacteria were isolated from waste samples collected from landfill site by using random sampling method under environmental temperature of 10oC. By using serial dilution and spread plate technique a total of 8 morphologically different psychrotrophic proteolytic bacteria were isolated on skim milk agar media at pH of 7.0 and temperature of 10°C after 48hours. Under in-vitro conditions all the isolates produced significant quantities of protease over the control and diameters of hydrolysis zones ranged between 2 to 18 mm at temperature range of 5 to 20oC and after 72 hours. The corresponding quantitative protease activities of the isolates was significant that ranged between 0.5 to 2.25 U/ml and the isolate PB2 was most efficient with highest protease activity of 2.25U/ml at 20oC. Based on 16SrRNA analysis the isolate was identified as Pseudomonas florescence with 96% similarity. It was concluded that the isolates can grow in wide ranges of temperature and could be used for enhanced decomposition of organic wastes during lower temperature conditions in cold regions. Further the isolates could have industrial applications due to the production of cold active proteases that would help economic benefits through energy conservation.

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confidence: 99%

Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

Hamid

Jehangir

Baba

et al. 2019

Preprint

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“…The second protease production media subjected for protease production was the sterilized remaining part of homogenized fish juice. The third production media used for protease production was 90 ml production media mixed with 10 ml sterilized homogenized fish juice (Nurullah, 2012).…”

Section: Protease Productionmentioning

confidence: 99%

Production of Proteolytic Enzyme from Catfish Waste Using Bacillus subtilis, Proteus vulgaris, and Arthrobacter aurescens

Fajingbesi

2017

Fountain Journal of Natural and Applied Sciences

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The world marine captured fishes contribute to more than 50% of the total world fish production. About 70% of fish is processed before final sale, resulting in 20-80% of fish waste depending on the level of processing and type of fish. The majority of fish wastes are disposed of in the ocean as well as on land. The discards from the processing plants amount to 20 million tonnes, which is equivalent to 25% of the worldâ€™s total production from marine captured fisheries. The fish processing industry generates considerable amounts of by-products such as waste that includes shells, scales, fins and bone frames. Interestingly, they are all often high in protein, which can be processed into useful products. Three organisms Bacillus subtilis, Proteus vulgaris, and Arthrobacter aurescens were isolated and used in the production of the proteolytic enzyme in three different protease production media. B. subtilis, P. vulgaris and A. aurescens were observed to have produced protease best at 48 hr of the second production, 72hr of the third production, and 72hr of first production, respectively. Fish waste should not be discarded into the environment, which can cause adverse effect on human health and environmental pollution, but rather they can be used in industries such as food industry in the production of baby foods to help break down the available protein into amino acids required by the body.

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“…Similar results were observed in reports on the optimization of protease production by B. subtilis. 32,33) The effects of agitation rates on cell count, pH value, and fibrinolytic activity are shown in Table 3. Agitation significantly increased cell count and pH value, although there was no significant difference among agitation rates tested.…”

Section: Evaluation Of Fibrinolytic Activity Of Various Seaweeds Fermmentioning

confidence: 99%

Production of Bacillus subtilis-fermented red alga Porphyra dentata suspension with fibrinolytic and immune-enhancing activities

Lin

Hwang

Lin

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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The fermented marine alga Porphyra dentata suspension was tested for its fibrinolytic and immune-enhancing activities. An isolated Bacillus subtilis N2 strain was selected for its fibrinolytic activity on fibrin plates. After investigating the effects of biomass amounts of P. dentata powder in water, various additives including sugars, nitrogen-containing substances, lipids and minerals, and cultural conditions of temperature and agitation in flask, the highest fibrinolytic activity in the cultural filtrate was obtained by cultivating N2 strain in 3% (w/v) P. dentata powder suspension containing 1% peanut oil at 37 °C, 150 rpm for 48 h. A fermentor system was further established using the same medium with controlled pH value of 7.0 at 37 °C, 150 rpm, 2.0 vvm for 48 h for the best fibrinolytic activity. The fermented product also showed its immune-enhancing activity by increasing cell proliferation and stimulating the secretion of IL-1β, IL-6, and TNF-α in J774.1 cells.

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Production of extracellular protease in submerged fermentation by Bacillus licheniformis ATCC 12759

Cited by 6 publications

References 38 publications

Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

Isolation and characterization of psychrotrophic proteolytic bacteria from landfill site under temperate climatic conditions of Kashmir Himalaya

Production of Proteolytic Enzyme from Catfish Waste Using Bacillus subtilis, Proteus vulgaris, and Arthrobacter aurescens

Production of Bacillus subtilis-fermented red alga Porphyra dentata suspension with fibrinolytic and immune-enhancing activities

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