Production of fertile transgenic lentil (Lens culinaris Medik) plants using particle bombardment

Gulati, Anju; Schryer, Pat; McHughen, Alan

doi:10.1079/ivp2002303

Cited by 45 publications

(26 citation statements)

References 33 publications

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“…The use of Agrobacterium tumefaciens as a vector for legume transformation was an important breakthrough. Both micro-particle bombardment (Gulati et al, 2002; and A. tumefaciens (De Clercq et al, 2002;Li et al, 2004) have been used for DNA delivery into either embryogenic or organogenic cultures. Transformation has been generally based on infection by A. tumefaciens, although A. rhizogenes is also used for transformation of some species to produce composite plants with hairy roots or hairy root cultures (BoissonDernier et al, 2001;Stiller et al, 1997;Wu & VanEtten, 2004).…”

Section: Genetic Transformationmentioning

confidence: 99%

Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

et al. 2006

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Biotic and abiotic stresses cause significant yield losses in legumes and can significantly affect their productivity. Biotechnology tools such as marker-assisted breeding, tissue culture, in vitro mutagenesis and genetic transformation can contribute to solve or reduce some of these constraints. However, only limited success has been achieved so far. The emergence of "omic" technologies and the establishment of model legume plants such as Medicago truncatula and Lotus japonicus are promising strategies for understanding the molecular genetic basis of stress resistance, which is an important bottleneck for molecular breeding. Understanding the mechanisms that regulate the expression of stress-related genes is a fundamental issue in plant biology and will be necessary for the genetic improvement of legumes. In this review, we describe the current status of biotechnology approaches in relation to biotic and abiotic stresses in legumes and how these useful tools could be used to improve resistance to important constraints affecting legume crops.

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Section: Genetic Transformationmentioning

confidence: 99%

Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

et al. 2006

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“…Although this approach is ethnically controversial, transformation technology is valuable not only for validation of gene function but also for planning for future direction of traditional breeding based on information obtained from transformants. Genetic modification mediated by Agrobacterium (Mahmoudian et al, 2002;Sarker et al, 2003;Warkentin et al, 1991Warkentin et al, , 1992 or bombardment (Gulati et al, 2002) has been attempted in lentil. However, these studies were mostly to establish transformation techniques rather than the introduction of genes into improved varieties.…”

Section: Development Of New Genetic Materialsmentioning

confidence: 99%

Application of biotechnology in breeding lentil for resistance to biotic and abiotic stress

et al. 2006

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“…Lentil seeds are very nutritious having protein levels ranging 20 -36% (Gulati et al 2002). In addition to its nutritive value, like other legume species, it has the ability to fix atmospheric nitrogen (Duranti and Gius 1997).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated Genetic Transformation in Lentil (Lens culinaris Medik.) followed by In vitro Flowering and Seed Formation

Das

Shethi

Hoque

et al. 2012

Plant Tissue Cult. & Biotech.

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Genetic transformation system was developed for two microsperma varieties of lentil (Lens culinaris Medik.), namely Bari Masur-4 (BM-4) and Bari Masur-5 (BM-5) using Agrobacterium tumefaciens strain LBA4404 harbouring binary plasmid pBI121, containing GUS and nptII genes. Three different types of embryo explants, namely cotyledonary node (CN), decapitated embryo (DE) and cotyledone attached decapitated embryo (CADE) were used. Highest GUS positive expression was found in DE followed by CADE as detected by transient assays. Following Agrobacterium infection CADE showed better response in developing multiple shoots on MS supplemented with 2.22 μM BAP, 2.32 μM Kn, 0.29 μM GA3 and 30.35 μM tyrosine. Selection of the transformed shoots was carried out by gradually increasing the concentration of kanamycin up to 200 mg/l. Transgenic lentil shoots were produced with an overall frequency of 1.009%. In vitro rooting appeared to have a limitation in obtaining complete plantlets in lentil, therefore in vitro flowering and seed formation were induced in transformed shoots of lentil with a view to recovering of the transgenic progenies. GUS positive shoots were found to produce in vitro flowers and pods on half-strength MS containing 98.4 μM IBA and 2.69 μM NAA. Expression of gene was detected in various tissues of the transformed shoots. Stable integration of GUS gene was also confirmed through PCR analysis.

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Production of fertile transgenic lentil (Lens culinaris Medik) plants using particle bombardment

Cited by 45 publications

References 33 publications

Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

Application of biotechnology in breeding lentil for resistance to biotic and abiotic stress

Agrobacterium-mediated Genetic Transformation in Lentil (Lens culinaris Medik.) followed by In vitro Flowering and Seed Formation

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