Production of fibrogenic cytokines by cord blood–derived cultured human mast cells

Kanbe, Naotomo; Kurosawa, Motohiro; Nagata, Hiroshi; Yamashita, Tetsuji; Kurimoto, Fumihiko

doi:10.1067/mai.2000.106777

Cited by 47 publications

(29 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent investigations [17,20,32,33] have demonstrated that increased numbers of mast cells detected by immunohictochemical survey of tryptase correlate with angiogenesis assessed by MVCs and poor outcome in patients with NSCLC, especially lung adenocarcinoma. Mast cells have been shown to produce angiogenic factors, including VEGF [16] and bFGF [19]. Mast cells have been reported to express MMPs [9,12,36,37], which are pivotal enzymes in matrix remodeling in the early stages of angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Mast cells produce endopeptidases such as tryptase and chymase [3,21,28,39], which show angiogenic activities [4,13,22]. Mast cells also produce angiogenic factors such as vascular endothelial growth factor (VEGF) [16] and basic fibroblast growth factor (bFGF) [19]. Increased numbers of mast cells detected with immunoreactivity for tryptase have been shown in malignant tumors, including nonsmall cell lung carcinoma (NSCLC) [17,20,32,33].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chymase-positive mast cells in small sized adenocarcinoma of the lung

et al. 2003

View full text Add to dashboard Cite

Mast cells accumulate in angiogenesis-dependent situations of lung adenocarcinoma. Human mast cells are divided into two major subsets: MCT (mast cells with immunoreactivity for tryptase but not chymase) and MCTC (reactive for tryptase and chymase). Chymase is an important mediator of tissue remodeling, but research into chymase-containing mast cell subpopulations has been hampered by the lack of reagents suitable for use with formalin-fixed tissue. We stained chymase using CC1 antibody in 66 cases of small sized lung adenocarcinoma as well as CD34 and tryptase. There were significant positive correlations of microvessel counts with MCT-type and MCTC-type mast cell counts in lung adenocarcinomas. When analyzed according to Noguchi's classification, MCT-type and MCTC-type mast cells were significantly increased in Noguchi type-C tumors [localized bronchioloalveolar carcinoma (LBAC) with active fibroblastic proliferation] compared with in Noguchi type-A (LBAC) plus type-B tumors (LBAC with alveolar collapse). Members in the high-count group of MCTC-type but not MCT-type mast cells showed a significantly worse outcome than those in the low-count group in LBACs. Counting chymase-positive (MCTC-type) mast cells in tumor stroma may be a good prognosis predictor for LBACs, especially Noguchi type-C tumors.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chymase-positive mast cells in small sized adenocarcinoma of the lung

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Certainly, in both human and animal models of post-traumatic contracture, joint capsule levels of TGF-b1 protein and mRNA are chronically elevated [12]. A variety of cell types have been shown to produce TGF-b1, including platelets, macrophages, neutrophils, activated fibroblasts and mast cells [31,32]. Compared to unaffected joint capsule specimens, which are sparsely populated by fibroblasts, numerous studies from our research group have proven the fibrotic post-traumatic joint capsule to be quite hypercellular, with mast cells and myofibroblasts representing roughly 90% of the total cell population [18,30].…”

Section: Discussionmentioning

confidence: 99%

The mast cell stabilizer ketotifen reduces joint capsule fibrosis in a rabbit model of post-traumatic joint contractures

et al. 2011

View full text Add to dashboard Cite

show abstract

“…By using in vitro-cultured human mast cells, we have been able to identify several new aspects of human mast cells, for example, the effect of IL-4 on human mast cells. [38][39][40] We also hypothesized that human mast cells participate in tissue remodeling by releasing fibrosis-induced mediators and cytokines 41 as well as produce enzymes which degenerate the extracellular matrix, known as metalloproteases. 42 However, to study the functional roles and developmental mechanism of mast cells in humans, the establishment of an appropriate in vivo model was needed.…”

Section: Discussionmentioning

confidence: 99%

Development of both human connective tissue-type and mucosal-type mast cells in mice from hematopoietic stem cells with identical distribution pattern to human body

Kambe

Hiramatsu

Shimonaka

et al. 2004

Blood

Self Cite

View full text Add to dashboard Cite

The transplantation of primitive human cells into sublethally irradiated immunedeficient mice is the well-established in vivo system for the investigation of human hematopoietic stem cell function. Although mast cells are the progeny of hematopoietic stem cells, human mast cell development in mice that underwent human hematopoietic stem cell transplantation has not been reported. Here we report on human mast cell development after xenotransplantation of human hematopoietic stem cells into nonobese diabetic severe combined immunodeficient (NOD/SCID)/␥ c null (NOG) mice with severe combined immunodeficiency and interleukin 2 (IL-2) receptor ␥-chain allelic mutation. Supported by the murine environment, human mast cell clusters developed in mouse dermis, but they required more time than other forms of human cell reconstitution. In lung and gastric tract, mucosal-type mast cells containing tryptase but lacking chymase located on gastric mucosa and in alveoli, whereas connective tissue-type mast cells containing both tryptase and chymase located on gastric submucosa and around major airways, as in the human body. Mast cell development was also observed in lymph nodes, spleen, and peritoneal cavity but not in the peripheral blood. Xenotransplantation of human hematopoietic stem cells into NOG mice can be expected to result IntroductionMast cells are recognized as the principal cells which initiate immunoglobulin E (IgE)-dependent immediate hypersensitivity and also as the cells which contribute to innate immunity and tissue remodeling. 1,2 There are 2 phenotypically distinct mast cell subpopulations in rodents: connective tissue-type mast cells (CTMCs) and mucosal-type mast cells (MMCs). These populations differ in location, cell size, staining characteristics, ultrastructure, mediator content, and T-cell dependency. 3 Proliferation of rodent MMCs is dependent on T-cell-derived cytokines, 3,4 whereas that of CTMCs is supported by stem cell factor (SCF). In humans, mast cells are distinguished on the basis of their protease composition, 5,6 MC TC contains tryptase and chymase in its granules and is predominant in skin and intestinal submucosa, like CTMCs in rodents. MC T also contains tryptase, but lacks chymase, and is predominant in the alveolar wall and gastric mucosa, similar to MMCs in rodents. Human mast cells were reported to develop only under the influence of SCF, but T-cell-derived interleukin 3 (IL-3) has little affect on their differentiation. 7 Recently, human intestinal mast cells were reported to respond to IL-3 by enhancing their growth, 8 but SCF is still an indispensable factor for human mast cells. Mast cells are the progenies of hematopoietic stem cells (HSCs). 9,10 In mice, the progenitor cells capable of becoming mast cells leave the bone marrow and enter the circulation but complete their differentiation into mast cells only after arriving in peripheral tissues such as lung, bowel, and skin. 10,11 Unfortunately, the developmental mechanism of human mast cells remains far less clear, possibly bec...

show abstract

Production of fibrogenic cytokines by cord blood–derived cultured human mast cells

Cited by 47 publications

References 22 publications

Chymase-positive mast cells in small sized adenocarcinoma of the lung

Chymase-positive mast cells in small sized adenocarcinoma of the lung

The mast cell stabilizer ketotifen reduces joint capsule fibrosis in a rabbit model of post-traumatic joint contractures

Development of both human connective tissue-type and mucosal-type mast cells in mice from hematopoietic stem cells with identical distribution pattern to human body

Contact Info

Product

Resources

About