Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules1

Kita, K; Watanabe, Takeshi; Ohsaka, Kimito; Hayashi, Hirofumi; Kubota, Yoshinobu; Nagashima, Yoji; Aoki, Ichiro; Taniguchi, Hideki; Noce, Toshiaki; Inoue, Kimiko; Miki, Hiromi; Ogonuki, Narumi; Tanaka, Hiromitsu; Ogura, Atsuo; Ogawa, Takehiko

doi:10.1095/biolreprod.106.056895

Cited by 87 publications

(94 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We showed earlier that spermatogenesis of mice and rats can take place in seminiferous tubules, which were reconstituted subcutaneously from dissociated embryonic or neonatal testicular cells [18]. We found that, once dissociated into single cells, a few testicular germ cells were integrated again in the reconstituted seminiferous tubules.…”

Section: Discussionmentioning

confidence: 64%

“…The integrated GS cells differentiated to round spermatids. Such spermatids were shown to be fertilization-competent by microinsemination into oocytes to deliver normal pups [18].…”

Section: Discussionmentioning

confidence: 99%

“…We also tested whether such reconstitution of seminiferous tubules is possible in other animal species by using porcine testicular cells. The dissociated neonatal porcine cells formed reorganized tubular structures in nude mice [18].…”

Section: Introductionmentioning

confidence: 99%

“…The mouse germ-line stem (GS) cells [16,17], which are cultured spermatogonial stem cells, were integrated in reconstituted tubules and were shown to undergo spermatogenesis up to the spermatid stage. The resultant spermatids were fertility-competent, and progeny were produced by microinsemination [18]. We also tested whether such reconstitution of seminiferous tubules is possible in other animal species by using porcine testicular cells.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods

Watanabe¹,

Hayashi²,

Kita³

et al. 2009

Asian J Androl

Self Cite

View full text Add to dashboard Cite

Fragments of testis tissue from immature animals grow and develop spermatogenesis when grafted onto subcutaneous areas of immunodeficient mice. The same results are obtained when dissociated cells from immature testes of rodents are injected into the subcutis of nude mice. Those cells reconstitute seminiferous tubules and facilitate spermatogenesis. We compared these two methods, tissue grafting and cell-injection methods, in terms of the efficiency of spermatogenesis in the backs of three strains of immunodeficient mice, using neonatal porcine testicular tissues and cells as donor material. Nude, severe combined immunodeficient (SCID) and NOD/Shi-SCID, IL-2Rγ c null (NOG) mice were used as recipients. At 10 months after surgery, the transplants were examined histologically. Both grafting and cell-injection methods resulted in porcine spermatogenesis on the backs of recipient mice; the percentage of spermatids present in the transplants was 67% and 22%, respectively. Using the grafting method, all three strains of mice supported the same extent of spermatogenesis. As for the cellinjection method, although SCID mice were the best host for supporting reconstitution and spermatogenesis, any difference from the other strains was not significant. As NOG mice did not show any better results, the severity of immunodeficiency seemed to be irrelevant for supporting xeno-ectopic spermatogenesis. Our results confirmed that tubular reconstitution is applicable to porcine testicular cells. This method as well as the grafting method would be useful for studying spermatogenesis in different kinds of animals.

show abstract

Section: Discussionmentioning

confidence: 64%

“…The integrated GS cells differentiated to round spermatids. Such spermatids were shown to be fertilization-competent by microinsemination into oocytes to deliver normal pups [18].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods

Watanabe¹,

Hayashi²,

Kita³

et al. 2009

Asian J Androl

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nonetheless, it has not yet been possible to induce spermatogenesis from these cells in vitro. To produce sperm from GS cells, we had to resort to systems using in vivo conditions such as cell transplantation in the seminiferous tubules 13 , or a method using the reconstructive nature of immature testicular cells 14 . These in vivo methods are useful for certain purposes, such as to test the functional properties of SSCs.…”

mentioning

confidence: 99%

In vitro production of fertile sperm from murine spermatogonial stem cell lines

et al. 2011

Self Cite

View full text Add to dashboard Cite

spermatogonial stem cells (ssCs) are the only stem cells in the body that transmit genetic information to the next generation. The long-term propagation of rodent ssCs is now possible in vitro, and their genetic modification is feasible. However, their differentiation into sperm is possible only under in vivo conditions. Here we show a new in vitro system that can induce full spermatogenesis from ssC lines or any isolated ssCs. The method depends on an organ culture system onto which ssCs are transplanted. The settled ssCs form colonies and differentiate up into sperm. The resultant haploid cells are fertile, and give rise to healthy offspring through micro-insemination. In addition, the system can induce spermatogenesis from ssCs that show spermatogenic failure due to a micro-environmental defect in their original testes. Thus, an in vitro system is established that can be used to correct or manipulate the micro-environmental conditions required for proper spermatogenesis from murine ssC lines.

show abstract

Current approaches for the treatment of male infertility with stem cell therapy

Pourmoghadam

Aghebati‐Maleki

Motalebnezhad

et al. 2018

Journal Cellular Physiology

View full text Add to dashboard Cite

Infertility is the inability to conceive after having regular unprotected sexual intercourse for more than 12 months and it can be caused by reproductive problems of men, women, or both, but sometimes the cause of infertility is unknown. At least 30 million men around the world suffer from infertility, most of whom are in Africa and Eastern Europe. Since infertility problems can have devastating emotional effects on couples as well as negative effects on the society, treatment of infertility is very important, as a result of which many studies have been carried out in this field and many treatments have been proposed. Recently, due to several advances in infertility treatments, more than 80% of infertile couples now have children. Among these treatments, stem cells as undifferentiated cells and because of their self-renewing and high differentiating potential have been considered by researchers. This review explained different types of male infertility and various therapies, with emphasis on stem cells therapy.

show abstract

Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules1

Cited by 87 publications

References 27 publications

Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods

Ectopic porcine spermatogenesis in murine subcutis: tissue grafting versus cell-injection methods

In vitro production of fertile sperm from murine spermatogonial stem cell lines

Current approaches for the treatment of male infertility with stem cell therapy

Contact Info

Product

Resources

About