Development of G0 potato seeds in medium altitude with high temperatures has been constrained high temperatures, which inhibited plant growth and development, especially for tuber formation. The production of G0 potato seeds in the high-temperature area can be increased by the application of nitrogen (N) fertilizer and paclobutrazol (PBZ). The study aimed to identify the effect of different doses of N-fertilizer and the frequency of PBZ application on the plant growth and yield of first-generation potato seeds (G0) in medium land. The Randomized Complete Block Design with two factors was used in this experiment. The first factor was the dose of N-fertilizer (n
1 = 60, n
2 = 120, and n
3 = 180 kg/ha) and the second factor was the frequency of PBZ application (p
1 = 1, p
2 = 2, and p
3 = 3 times), applied starting at 40 DAP and repeated every 10 days with a concentration of 100 ppm. The results showed that there was a significant interaction between the dose of N-fertilizer and the frequency of PBZ application on plant dry weight. The 120 kg/ha of N-fertilizer-treated plants exhibited the highest plant height and the number of stolons per plant, whereas three times PBZ application treated plant exhibited the higher tuber growth rate, the number, and weight of tuber per plant with the value of number and weight of tuber per plant of 9.26 and 83.97 g, respectively. It can be concluded that N-fertilizer increased plant growth, whereas PBZ increased G0 potato seeds in the medium land altitude with high temperature.