Production of gamma-aminobutyric acid by Lactobacillus brevis NCL912 using fed-batch fermentation

Abstract

BackgroundGamma-aminobutyric acid is a major inhibitory neurotransmitter in mammalian brains, and has several well-known physiological functions. Lactic acid bacteria possess special physiological activities and are generally regarded as safe. Therefore, using lactic acid bacteria as cell factories for gamma-aminobutyric acid production is a fascinating project and opens up a vast range of prospects for making use of GABA and LAB. We previously screened a high GABA-producer Lactobacillus brevis NCL912 and optimized its fermentation medium composition. The results indicated that the strain showed potential in large-scale fermentation for the production of gamma-aminobutyric acid. To increase the yielding of GABA, further study on the fermentation process is needed before the industrial application in the future. In this article we investigated the impacts of pyridoxal-5'-phosphate, pH, temperature and initial glutamate concentration on gamma-aminobutyric acid production by Lactobacillus brevis NCL912 in flask cultures. According to the data obtained in the above, a simple and effective fed-batch fermentation method was developed to highly efficiently convert glutamate to gamma-aminobutyric acid.ResultsPyridoxal-5'-phosphate did not affect the cell growth and gamma-aminobutyric acid production of Lb. brevis NCL912. Temperature, pH and initial glutamate concentration had significant effects on the cell growth and gamma-aminobutyric acid production of Lb. brevis NCL912. The optimal temperature, pH and initial glutamate concentration were 30-35°C, 5.0 and 250-500 mM. In the following fed-batch fermentations, temperature, pH and initial glutamate concentration were fixed as 32°C, 5.0 and 400 mM. 280.70 g (1.5 mol) and 224.56 g (1.2 mol) glutamate were supplemented into the bioreactor at 12 h and 24 h, respectively. Under the selected fermentation conditions, gamma-aminobutyric acid was rapidly produced at the first 36 h and almost not produced after then. The gamma-aminobutyric acid concentration reached 1005.81 ± 47.88 mM, and the residual glucose and glutamate were 15.28 ± 0.51 g L-1 and 134.45 ± 24.22 mM at 48 h.ConclusionsA simple and effective fed-batch fermentation method was developed for Lb. brevis NCL912 to produce gamma-aminobutyric acid. The results reveal that Lb. brevis NCL912 exhibits a great application potential in large-scale fermentation for the production of gamma-aminobutyric acid.

Production of GABA (gamma amino butyric acid) by Lactic Acid Bacteria

“…In China, it was reported that 1,000 mM levels of GABA has been produced by fed-batch recently (Li et al, 2010). Accordingly, the production of GABA has been increased by screening of a new strain and optimization of fermentation conditions, and the various applications of purposes are possible.…”

“…It succeeded to produce 1005.8 mM of GABA from 3.1 M MSG by Lb. brevis NCL912 isolated from Paochai in China (Li et al, 2010).…”

BacHBerry: BACterial Hosts for production of Bioactive phenolics from bERRY fruits

“…Furthermore, the fed-batch fermentation mode, where one or more nutrient is being supplied throughout cultivation (Moulton 2014), can be done in a controllable and reproducible fashion only in bioreactors. These properties make bioreactors a suitable stage for optimization of cultivation conditions, often leading to significant improvements in production yields (Hujanen et al 2001;Ratnam et al 2003;Li et al 2010;Rani and Appaiah 2011).…”

Development of bio-based fine chemical production through synthetic bioengineering

“…GABA, which was originally identified in traditional fermented foods such as cheese, yogurt [5] and kimchi [6], is synthesized through the alphadecarboxylation of L-glutamate catalyzed by glutamate decarboxylase (GAD, EC 4.1.1.15) [2]. GABA is produced by lactic acid bacteria (LABs) such as Lactobacillus paracasei [7], L. buchneri [6], and L. brevis [8,9] (Table 2), and the latter produces high yields of GABA through fedbatch processes [10].…”