Production of ginsenoside aglycone (protopanaxatriol) and male sterility of transgenic tobacco co-overexpressing three Panax ginseng genes: PgDDS, CYP716A47, and CYP716A53v2

Gwak, Yu Shin; Han, Jung Yeon; Choi, Yong Eui

doi:10.1016/j.jgr.2018.02.005

Cited by 10 publications

(3 citation statements)

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“…In 2011, Han [ 40 ] co-expressed PgDDS and PgCYP716A47 in yeast and found that protopanaxadiol-type sapogenins (PPDs) could be produced without the addition of dammarendiol and verified that CYP716A47 could catalyze the production of PPD from dammarendiol by in vitro enzymatic activity assays. In 2019, Gwak [ 41 ] co-transformed PgDDS , PgCYP716A47 and PgCYP716A53v2 ( PgCYP137 ) into tobacco and produced protopanaxatriol-type sapogenins (PPT) in transgenic tobacco, indicating that CYP716A53v2 can catalyze PPD to produce PPT. In 2014, Yan [ 42 ] heterologously expressed the UGTPg1 ( PgUGT1 ) gene in yeast and found that the UGTPg1-encoded protein could catalyze the production of rare ginsenoside CK from PPD as a substrate, as well as another novel compound, 20S-O-β-(D-glucosyl)-dammarenediol II (DMG).…”

Section: Discussionmentioning

confidence: 99%

The AP2/ERF Transcription Factor PgERF120 Regulates Ginsenoside Biosynthesis in Ginseng

Jiang,

Zhang,

Zeng

et al. 2024

Biomolecules

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Ginseng (Panax ginseng C.A. Meyer) is a perennial herb belonging to the family Araliaceae and has been used for thousands of years in East Asia as an essential traditional medicine with a wide range of pharmacological activities of its main active ingredient, ginsenosides. The AP2/ERF gene family, widely present in plants, is a class of transcription factors capable of responding to ethylene regulation that has an influential role in regulating the synthesis of major active ingredients in medicinal plants and in response to biotic and abiotic stresses, which have not been reported in Panax ginseng. In this study, the AP2/ERF gene was localized on the ginseng chromosome, and an AP2/ERF gene duplication event was also discovered in Panax ginseng. The expression of seven ERF genes and three key enzyme genes related to saponin synthesis was measured by fluorescence quantitative PCR using ethylene treatment of ginseng hairy roots, and it was observed that ethylene promoted the expression of genes related to the synthesis of ginsenosides, among which the PgERF120 gene was the most sensitive to ethylene. We analyzed the sequence features and expression patterns of the PgERF120 gene and found that the expression of the PgERF120 gene was specific in time and space. The PgERF120 gene was subsequently cloned, and plant overexpression and RNA interference vectors were constructed. Ginseng adventitious roots were transformed using the Agrobacterium tumefaciens-mediated method to obtain transgenic ginseng hairy roots, and the gene expression, ginsenoside content and malondialdehyde content in overexpression-positive hairy roots were also analyzed. This study preliminarily verified that the PgERF120 gene can be involved in the regulation of ginsenoside synthesis, which provides a theoretical basis for the study of functional genes in ginseng and a genetic resource for the subsequent use of synthetic biology methods to improve the yield of ginsenosides.

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Section: Discussionmentioning

confidence: 99%

The AP2/ERF Transcription Factor PgERF120 Regulates Ginsenoside Biosynthesis in Ginseng

Jiang,

Zhang,

Zeng

et al. 2024

Biomolecules

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“…An enzyme gene CYP6H (MZ27754) belonging to cytochrome P450 family was screened out from P. notoginseng. Bioinformatic analysis of CYP6H showed that CYP6H had high homology with CYP716A53V2, the nucleic acid similarity between the two genes is 94.88%, and the enzyme encoded by CYP716A53V2 could hydroxylated protopanaxadiol to protopanaxatriol (Gwak et al 2019). So, CYP6H might has the similar function as CYP716A53V2.…”

Section: Construction Of Transgenic Tobaccos For Protopanaxatriol Syn...mentioning

confidence: 99%

Constructing a Biosynthetic Pathway of Ginsenoside F1 to Achieve Its Synthesis in Tobacco

Chen,

Lei,

et al. 2023

Preprint

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Ginsenoside F1 has high medicinal values, which is a kind of rare triterpene saponin isolated from Panax plants. The extremely low content of ginsenoside F1 in herbs has limited its research and application in medical field. In this work, we constructed a pathway in tobacco for the biosynthesis of ginsenoside F1 by metabolic engineering. Four enzyme genes (PnDDS, CYP12H, CYP6H and UGT20) isolated from Panax notoginseng were introduced into tobacco. Thus, a biosynthetic pathway for ginsenoside F1 synthesis was artificially constructed in tobacco cells; moreover, the four exogenous genes could be expressed in the roots, stems and leaves of transgenic plants. Consequently, ginsenoside F1 and its precursors were successfully synthesized in the transgenic tobacco, compared with Panax plants, the content of ginsenoside F1 in transgenic tobacco was doubled. Based on these results, a new approach was established to produce rare ginsenoside F1; meanwhile, such strategy could also be employed in plant hosts for the heterologous synthesis of other important or rare natural products.

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“…cell suspension culture Plant Cell Fermentation (PCF) Technology https://phytonbiotech.com/apis/paclitaxel/ Taxol Taxus spp. cell suspension culture Samyang’s plant cell culture technology https://www.samyangbiopharm.com/eng/ Triterpenoids Astragaloside A. membranaceus hairy root Elicitors ( Jiao et al., 2016 ) Diosgenin N. benthamiana Pooled screens and Overexpression ( Christ et al., 2019 ) Dammarenediol-II N. tabacum Overexpression ( Han et al., 2014a ) Ginsenoside P. ginseng www.nitto.com Protopanaxatriol/protopanaxadiol N. tabacum Overexpression ( Chun et al., 2015 ; Gwak et al., 2019 ) Squalene N. tabacum Compartmentalized metabolic engineering ( Pasoreck et al., 2016 ) Triterpene N. benthamiana Combinatorial transformation ( Reed et al., 2017 ) Triterpenoids C. asiatica hairy root Elicitors ( Baek et al., 2020 ) Tetraterpenoids β -Carotene Rice Overexpression ( Ye et al., 2000 ) β -Carotene Tomato Overexpression ( Apel and Bock, 2009 ) β -Carotene/carotenoids Maize Combinatorial transformation ( Zhu et al., 2008 ) Carotenoid Potato Overexpression ...…”

Section: Introductionmentioning

confidence: 99%

Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells

Zhu

Liu

et al. 2021

Plant Communications

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Plant natural products (PNPs) are the main sources of drugs, food additives, and new biofuels and have become a hotspot in synthetic biology. In the past two decades, the engineered biosynthesis of many PNPs has been achieved through the construction of microbial cell factories. Alongside the rapid development of plant physiology, genetics, and plant genetic modification techniques, hosts have now expanded from single-celled microbes to complex plant systems. Plant synthetic biology is an emerging field that combines engineering principles with plant biology. In this review, we introduce recent advances in the biosynthetic pathway elucidation of PNPs and summarize the progress of engineered PNP biosynthesis in plant cells. Furthermore, a future vision of plant synthetic biology is proposed. Although we are still a long way from overcoming all the bottlenecks in plant synthetic biology, the ascent of this field is expected to provide a huge opportunity for future agriculture and industry.

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Production of ginsenoside aglycone (protopanaxatriol) and male sterility of transgenic tobacco co-overexpressing three Panax ginseng genes: PgDDS, CYP716A47, and CYP716A53v2

Cited by 10 publications

References 52 publications

The AP2/ERF Transcription Factor PgERF120 Regulates Ginsenoside Biosynthesis in Ginseng

The AP2/ERF Transcription Factor PgERF120 Regulates Ginsenoside Biosynthesis in Ginseng

Constructing a Biosynthetic Pathway of Ginsenoside F1 to Achieve Its Synthesis in Tobacco

Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells

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