Production of Glucose Isomerase by Streptomyces flavogriseus

Abstract

A microorganism that produces glucose isomerase was isolated from soil and identified as a strain of Streptomyces flavogriseus. The organism produced a large quantity of glucose isomerase when grown on straw hemicellulose, xylan, xylose, and H2SO4 hydrolysate of ryegrass straw. The organism produced glucose isomerase both intra-and extra-cellularly. The highest level of intracellular glucose isomerase (3.5 U/ml) was obtained in about 36 h by a culture grown on straw hemicellulose; the extracellular enzyme (1.5 U/ml) appeared in cultures grown for about 72 h. About equal levels of enzyme were produced in cultures grown on straw hemicellulose, xylan, xylose, and H2SO4 hydrolysate of straw, but production of the enzyme was drastically reduced when the organism was grown on other carbon sources. As a nitrogen source, corn steep liquor produced the best results. Soy flour extract, yeast extract, and various peptones also were adequate substrates for g!ucose isomerase production. Addition of Mg2+, Mn2+, or Fe2" to the growth medium significantly enhanced enzyme production. The organism, however, did not require Co2+, which is commonly required by microorganisms used in the production of glucose isomerase.

Molecular and industrial aspects of glucose isomerase.

“…Reports on extracellular secretion of GI are not common. Extracellular GI has been reported to be produced by Streptomyces glaucescens (182) and S. flavogriseus (45), for which the release of the enzyme from the cells was attributed to a change in the cell wall permeability and partial lysis of the cells. The extracellular xylose isomerases from Chainia sp.…”

Brewer’s spent grain and corn steep liquor as substrates for cellulolytic enzymes production byStreptomyces malaysiensis

“…This cheap residue has been successfully used for a variety of fermentations such as solvent production and antibiotics (De Azeredo et al 2006). It has also been used in some culture media, in glucose isomerase (Chen et al 1979), cellulase (Lima et al 2005) and protease (De Azeredo et al 2006) production.…”

Peer Review #1 of "Identification and characterization of Streptomyces flavogriseus NJ-4 as a novel producer of actinomycin D and holomycin (v0.1)"

“…Streptomyces species are soil bacteria, from which numerous antibiotic and antifungal compounds have been derived (Weber, 2015). Streptomyces flavogriseus has been reported to produce several enzymes, such as exoglucanase, extracellular proteases, cellulase, xylanase, and glucose isomerase (Chen et al, 1979;Mackenzie et al, 1984;Ghorbel et al, 2014), and antibiotics, such as the polyketide antitumour antibiotic xantholipin, the polycyclic xanthone bromoxantholipin, epithienamycin and xanthomycin (Chen et al, 2011;Zhang et al, 2012;Arasu et al, 2013). However, no public data have been found demonstrating that S. flavogriseus can produce actinomycin D or holomycin.…”