Production of glycogen, PHB, biohydrogen, NAD(P)H, and proteins in Synechocystis sp. PCC 6803 disrupted in metabolically linked biosynthetic pathway(s)

Kaewbai-ngam, Janine; Sukkasam, Nannaphat; Phoraksa, Onuma; Incharoensakdi, Aran; Monshupanee, Tanakarn

doi:10.1007/s10811-022-02759-2

Cited by 5 publications

(2 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B12 under high-light and N depletion. In particular, N starvation is known to induce chlorosis and protein degradation [ 21 , 26 , 97 ], because of the need to remobilize the N present in biomolecules such as pigments and amino acids [ 88 ] and to store energy in forms of reduced carbon molecules not containing N, such as glycogen and PHB. Synechocystis sp.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the genetic landscape of enhanced poly-3-hydroxybutyrate production in Synechocystis sp. B12

Santin,

Collura,

Singh

et al. 2024

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Microbial biopolymers such as poly-3-hydroxybutyrate (PHB) are emerging as promising alternatives for sustainable production of biodegradable bioplastics. Their promise is heightened by the potential utilisation of photosynthetic organisms, thus exploiting sunlight and carbon dioxide as source of energy and carbon, respectively. The cyanobacterium Synechocystis sp. B12 is an attractive candidate for its superior ability to accumulate high amounts of PHB as well as for its high-light tolerance, which makes it extremely suitable for large-scale cultivation. Beyond its practical applications, B12 serves as an intriguing model for unravelling the molecular mechanisms behind PHB accumulation. Results Through a multifaceted approach, integrating physiological, genomic and transcriptomic analyses, this work identified genes involved in the upregulation of chlorophyll biosynthesis and phycobilisome degradation as the possible candidates providing Synechocystis sp. B12 an advantage in growth under high-light conditions. Gene expression differences in pentose phosphate pathway and acetyl-CoA metabolism were instead recognised as mainly responsible for the increased Synechocystis sp. B12 PHB production during nitrogen starvation. In both response to strong illumination and PHB accumulation, Synechocystis sp. B12 showed a metabolic modulation similar but more pronounced than the reference strain, yielding in better performances. Conclusions Our findings shed light on the molecular mechanisms of PHB biosynthesis, providing valuable insights for optimising the use of Synechocystis in economically viable and sustainable PHB production. In addition, this work supplies crucial knowledge about the metabolic processes involved in production and accumulation of these molecules, which can be seminal for the application to other microorganisms as well.

show abstract

Section: Discussionmentioning

confidence: 99%

Deciphering the genetic landscape of enhanced poly-3-hydroxybutyrate production in Synechocystis sp. B12

Santin,

Collura,

Singh

et al. 2024

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…Various cyanobacteria accumulate PHB in the absence of essential nutrients including nitrogen or phosphorus ( Drosg et al, 2015 ; Koller and Maršálek, 2015 ; Kaewbai-ngam et al, 2016 ). The previous reports on Synechocystis suggested that PHB serves as a carbon and energy reserve for growth recovery under nitrogen repletion ( Koch et al, 2019 ; Kaewbai-ngam et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium <em>Synechocystis</em> sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography

Kaewbai-ngam,

Incharoensakdi,

Monshupanee

2023

BIO-PROTOCOL

Self Cite

View full text Add to dashboard Cite

Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HPLC analysis is rapid, with a running time of approximately 5 min per sample. The technique can accurately determine PHB concentrations in the range of 2–1,000 μg/mL PHB. However, this technique is not applicable for determining the contents of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in cyanobacteria.

show abstract

Bacterial Photosynthesis for Nutrient Recovery and Value Addition

Sreeharsha,

Venkata Mohan

2024

Microbial Photosynthesis

View full text Add to dashboard Cite

Production of glycogen, PHB, biohydrogen, NAD(P)H, and proteins in Synechocystis sp. PCC 6803 disrupted in metabolically linked biosynthetic pathway(s)

Cited by 5 publications

References 53 publications

Deciphering the genetic landscape of enhanced poly-3-hydroxybutyrate production in Synechocystis sp. B12

Deciphering the genetic landscape of enhanced poly-3-hydroxybutyrate production in Synechocystis sp. B12

Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium <em>Synechocystis</em> sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography

Bacterial Photosynthesis for Nutrient Recovery and Value Addition

Contact Info

Product

Resources

About